Disease relevance of Hmbox1

• Homeobox-containing genes in teratocarcinoma embryoid bodies: a possible role for Hox-D12 (Hox-4.7) in establishing the extraembryonic endoderm lineage in the mouse [1].
• Congenital megacolon develops in transgenic mice that overexpress the homeobox-containing gene, Hoxa-4 [2].
• AIM: To determine whether retrovirally expressed Dlx5, a homeobox-containing transcription factor, can induce a 2.3 kb rat COL1A1 promoter-reporter construct, which is transduced into osteoblastic cells by the use of a retrovirus vector [3].

High impact information on Hmbox1

• Pitx2, a member of the bicoid-related family of homeobox-containing genes, is asymmetrically expressed in the left lateral plate mesoderm and derived tissues during chick and mouse development [4].
• Hox-1.1 is a murine homeobox-containing gene expressed in a time- and cell-specific manner during embryogenesis [5].
• Hox-7.1, a homeobox-containing gene, is expressed throughout the limb bud when limb outgrowth begins, whereas transcripts are later restricted to distal limb mesenchyme which is the proposed site of positional specification [6].
• This is further supported by our finding that inactivation of Gbx2, a homeobox-containing gene expressed in the presomitic mesoderm but not in the somites, produced Hox-like phenotypes in the axial skeleton without affecting Hox gene expression [7].
• Vax1, a novel homeobox-containing gene, directs development of the basal forebrain and visual system [8].

Biological context of Hmbox1

• The murine Hox 3.1 gene maps to a cluster of homeobox-containing genes on chromosome 15 [9].
• Accumulation to high amounts of homeobox-containing gene transcripts in these experiments seems to correlate with retinoic acid-induced EC cell differentiation rather than with EC cell differentiation as such [10].
• These findings are consistent with the hypothesis that Evx-1, a homeobox-containing gene, may activate the cytotactin promoter by a mechanism involving a growth-factor signal transduction pathway [11].
• Genomic blot analysis shows that each of the homeobox-containing DNA segments is probably present in a single copy per haploid genome [12].
• This study reports the structure of the mouse homeobox-containing gene Hox-1.4 of the HOX-1 cluster, as well as its expression pattern during embryonic and fetal development [13].

Anatomical context of Hmbox1

• In situ hybridization on paraffin sections of mouse tissues demonstrated that Hmbox1 is widely expressed in pancreas and the expression of this gene can also be detected in pallium, hippocampus and hypothalamus [14].
• Gtx: a novel murine homeobox-containing gene, expressed specifically in glial cells of the brain and germ cells of testis, has a transcriptional repressor activity in vitro for a serum-inducible promoter [15].
• Hoxa-4 (previously known as Hox-1.4) is a mouse homeobox-containing gene that is expressed in the presumptive hindbrain and spinal cord, prevertebrae, and other tissues during embryogenesis [16].
• Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development [17].
• Gax is a homeobox-containing gene that has been detected in adult cardiovascular tissues and exhibits a growth arrest-specific pattern of expression in cultured vascular myocytes [18].

Associations of Hmbox1 with chemical compounds

• Induction to high levels of expression of several homeobox-containing genes during differentiation occurs only in the presence of retinoic acid [10].
• These data suggest that the pattern of Hox-D12 expression correlates with endoderm differentiation and not with RA treatment and point to a possible role for homeobox-containing genes during the early stages of mouse embryogenesis [1].
• To identify genes important for early development, we have constructed a genomic library from Atlantic salmon (Salmo salar) and screened it with homeobox-containing probes from Drosophila melanogaster [19].

Physical interactions of Hmbox1

• Menin interacts directly with the homeobox-containing protein Pem [20].
• In this report, we show that the Krox-20 protein binds in vitro to two specific DNA sites located upstream from the homeobox containing gene Hox-1 [21].

Other interactions of Hmbox1

• The homeobox-containing genes Msx1 and Msx2 are highly expressed in the limb field from the earliest stages of limb formation and, subsequently, in both the apical ectodermal ridge and underlying mesenchyme [22].
• Expression and mutation analyses in mice suggest that the homeobox-containing gene Engrailed (En) plays a role in dorsoventral patterning of the limb [23].
• The homeobox containing gene Lbx1 is required for correct dorsal-ventral patterning of the neural tube [24].
• We show here that a member of the Six family of homeobox containing transcription factors, namely Six2 activates Gdnf expression [25].
• Previous studies have shown that En-1, a homeobox-containing gene, maps close to or at the Dh locus in the mouse [26].

Analytical, diagnostic and therapeutic context of Hmbox1

• Using gene targeting, we have created mice with a disruption in the homeobox-containing gene hoxd-11 [27].
• Northern blot analysis of RNA transcripts indicates that the rat homeobox-containing sequences are expressed during embryogenesis and in newborn and adult tissues in a tissue-specific manner; RNA expression is predominantly detected in spinal cord and kidney [12].
• We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2 [28].
• To identify homeobox genes involved in mouse embryo development, we have screened a mouse cDNA library prepared from the 13.5-day-old entire conceptus for homeobox-containing sequences using a polymerase chain reaction (PCR) cloning strategy [29].
• METHODS: Functionally distinct subpopulations of murine bone marrow (BM) and fetal liver day 14.5 (FL) cells were isolated by flow cytometry, and gene expression of various homeobox-containing genes was assessed by global cDNA amplification technique [30].

References