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Gene Review

Camk1  -  calcium/calmodulin-dependent protein kinase I

Rattus norvegicus

Synonyms: CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, Calcium/calmodulin-dependent protein kinase type 1, ...
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High impact information on Camk1

  • Activity-dependent dendritic outgrowth and branching in cultured hippocampal neurons and slices is mediated through activation by CaM-dependent protein kinase kinase (CaMKK) of the membrane-associated gamma isoform of CaMKI [1].
  • These proteins were unphosphorylated and were bound only to the Thr177-phosphorylated CaM-KI catalytic domain [2].
  • Unlike wild-type CaM-KI, however, phosphorylation of Thr177 in the chimera mutants by CaM-KK resulted in generation of significant autonomous activities, indicating that the phosphorylation of Thr in the activation loop is sufficient to partially release the autoinhibitory region of CaM-KIV from the catalytic core [3].
  • We have shown that the kinetics of Thr196 phosphorylation of CaM-KIV by CaM-KK is well correlated with the generation of its autonomous activity, although Thr177 phosphorylation of CaM-KI does not induce its autonomous activity [3].
  • In situ hybridization analysis has demonstrated that CaMKI gamma mRNA is expressed in a distinct pattern from other isoforms of CaMKI with predominant expression in some restricted brain regions such as the olfactory bulb, hippocampal pyramidal cell layer of CA3, central amygdaloid nuclei, ventromedial hypothalamic nucleus and pineal gland [4].

Biological context of Camk1


Anatomical context of Camk1


Enzymatic interactions of Camk1

  • We recently cloned a calmodulin-dependent protein kinase kinase (CaM-KK) which phosphorylates and activates CaM-KI and CaM-KIV [Tokumitsu, H., Enslen, H., and Soderling, T. R. (1995) J. Biol. Chem. 270, 19320-19324] [9].

Regulatory relationships of Camk1


Other interactions of Camk1

  • These results identify CaMKI as a positive transducer of growth cone motility and axon outgrowth and provide a new physiological role for the CaMKK-CaMKI pathway [6].
  • Calcium/calmodulin-dependent protein kinase I-alpha (CaMKI-alpha) is a ubiquitous cytosolic enzyme that phosphorylates a number of nuclear proteins in vitro and has been implicated in transcriptional regulation [5].
  • Here, we have isolated and determined the complete primary structures of two alternatively splicing isoforms of CaMKI termed CaMKI gamma 1 and -gamma 2 [4].
  • Ca2+/calmodulin-dependent protein kinase I (CaMKI), originally identified as a protein kinase phosphorylating synapsin I, has been shown to constitute a family of closely related isoforms (alpha, beta and gamma) [4].
  • Transfection experiments using expression plasmids for constitutively active forms of CaMKI, CaMKII, or CaMKIV show that CaMKII is the most effective activator of prolactin promoter expression [10].

Analytical, diagnostic and therapeutic context of Camk1

  • To search for the substrates of Ca2+/calmodulin-dependent protein kinase I (CaM-KI), we performed affinity chromatography purification using either the unphosphorylated or phosphorylated (at Thr177) GST-fused CaM-KI catalytic domain (residues 1-293, K49E) as the affinity ligand [2].


  1. Activity-dependent dendritic arborization mediated by CaM-kinase I activation and enhanced CREB-dependent transcription of Wnt-2. Wayman, G.A., Impey, S., Marks, D., Saneyoshi, T., Grant, W.F., Derkach, V., Soderling, T.R. Neuron (2006) [Pubmed]
  2. Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. Tokumitsu, H., Hatano, N., Inuzuka, H., Sueyoshi, Y., Yokokura, S., Ichimura, T., Nozaki, N., Kobayashi, R. J. Biol. Chem. (2005) [Pubmed]
  3. Mechanism of the generation of autonomous activity of Ca2+/calmodulin-dependent protein kinase IV. Tokumitsu, H., Hatano, N., Inuzuka, H., Yokokura, S., Nozaki, N., Kobayashi, R. J. Biol. Chem. (2004) [Pubmed]
  4. Cloning, characterization and expression of two alternatively splicing isoforms of Ca2+/calmodulin-dependent protein kinase I gamma in the rat brain. Nishimura, H., Sakagami, H., Uezu, A., Fukunaga, K., Watanabe, M., Kondo, H. J. Neurochem. (2003) [Pubmed]
  5. Cytoplasmic localization of calcium/calmodulin-dependent protein kinase I-alpha depends on a nuclear export signal in its regulatory domain. Stedman, D.R., Uboha, N.V., Stedman, T.T., Nairn, A.C., Picciotto, M.R. FEBS Lett. (2004) [Pubmed]
  6. Regulation of axonal extension and growth cone motility by calmodulin-dependent protein kinase I. Wayman, G.A., Kaech, S., Grant, W.F., Davare, M., Impey, S., Tokumitsu, H., Nozaki, N., Banker, G., Soderling, T.R. J. Neurosci. (2004) [Pubmed]
  7. Characterization of Ca2+/calmodulin-dependent protein kinase I as a myosin II regulatory light chain kinase in vitro and in vivo. Suizu, F., Fukuta, Y., Ueda, K., Iwasaki, T., Tokumitsu, H., Hosoya, H. Biochem. J. (2002) [Pubmed]
  8. Demonstration of a Ca2+/calmodulin dependent protein kinase cascade in the hog heart. Uemura, A., Naito, Y., Matsubara, T., Hotta, N., Hidaka, H. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  9. Calcium/calmodulin-dependent protein kinase kinase: identification of regulatory domains. Tokumitsu, H., Wayman, G.A., Muramatsu, M., Soderling, T.R. Biochemistry (1997) [Pubmed]
  10. Characterization of DNA regions mediating the ability of Ca2+/calmodulin dependent protein kinase II to stimulate prolactin promoter activity. Nowakowski, B.E., Okimura, Y., Maurer, R.A. Mol. Cell. Endocrinol. (1997) [Pubmed]
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