Disease relevance of Phb

• Prohibitin has antiproliferative activity and available data suggest a role in such diverse processes as normal cell cycle regulation, replicative senescence, cellular immortalization, and the development of sporadic breast tumors [1].

High impact information on Phb

• We show that the CKGGRAKDC peptide associates with prohibitin, a multifunctional membrane protein, and establish prohibitin as a vascular marker of adipose tissue [2].
• Because prohibitin is also expressed in blood vessels of human white fat, this work may lead to the development of targeted drugs for treatment of obese patients [2].
• The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein [3].
• Since prohibitin was identified as an inhibitor of cell proliferation, its association with mIgM, but not mIgD, could explain the different biological events elicited after engagement of each receptor [3].
• The deduced amino acid sequence of p32 is identical to that of rat prohibitin. p37 is highly homologous to p32 [3].

Biological context of Phb

• The two prohibitin proteins, Phb1p and Phb2p(BAP37), have been ascribed various functions, including cell cycle regulation, apoptosis, assembly of mitochondrial respiratory chain enzymes, and aging [4].
• Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence [4].
• Prohibitin: potential role in senescence, development, and tumor suppression [1].
• Prohibitin (PHB-1) is a highly conserved protein involved in mitochondrial biogenesis and function [5].

Anatomical context of Phb

• A close association of mitochondria with lipid droplets was suggested by the identification of pyruvate carboxylase, prohibitin, and a subunit of ATP synthase in the preparations [6].
• In addition, differential expression studies of parent and transformed MCF-12F cell lines using microarrays showed that prohibitin mRNA was increased 4-fold in the transformed cells [7].
• All these proteins were up-regulated in the membranes of H-RasV12-transformed p38alpha-/-cells, except for prohibitin, which was down-regulated [8].

Associations of Phb with chemical compounds

• The Km for glutathione for the GSH-dependent dethiolase was 15 microM with Phb as substrate and 10 microM with CAIII [9].
• Prohibitin attenuates insulin-stimulated glucose and fatty acid oxidation in adipose tissue by inhibition of pyruvate carboxylase [5].

Other interactions of Phb

• Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins [10].
• Mass spectrometric analysis and Western blotting identified eight of them, including prohibitin, 14-3-3zeta, tropomyosin 3 and Hsp84 [11].

Analytical, diagnostic and therapeutic context of Phb

• Dissection of melanogenesis with small molecules identifies prohibitin as a regulator [12].

References