Disease relevance of EML1

• Fusion of EML1 to ABL1 in T-cell acute lymphoblastic leukemia with cryptic t(9;14)(q34;q32) [1].
• Multipoint analysis indicates that the canine disease gene lies in an interval between the echinoderm microtubule-associated, protein-like 1 ( EML1) gene and the telomere [2].
• Isolation of a novel human homologue of the gene coding for echinoderm microtubule-associated protein (EMAP) from the Usher syndrome type 1a locus at 14q32 [3].
• To carry out these experiments, we constructed a recombinant baculovirus that expressed the full-length 77-kD EMAP, an abundant MAP that localizes to the mitotic spindle of cleavage-stage sea urchin embryos and to the interphase array of microtubules in adult coelomocytes [4].

High impact information on EML1

• In this report, we show that the echinoderm microtubule (MT)-associated protein (EMAP) and related EMAP-like proteins (ELPs) share a similar domain organization with a highly conserved hydrophobic ELP (HELP) domain and a large tryptophan-aspartic acid (WD) repeat domain [5].
• At all stages of the cell cycle, a 33 kDa polypeptide copurifies with the 77 kDa EMAP, regardless of phosphorylation state [6].
• As the embryo enters mitosis, EMAP phosphorylation increases, and as the embryo exits mitosis, phosphorylation decreases [6].
• In the unfertilized egg, EMAP is constitutively phosphorylated on at least five serine residues [6].
• We subsequently stabile-transfected this cell line with a retroviral construct containing the EMAP gene [7].

Biological context of EML1

• The nucleotide sequence of the cDNA encodes a protein of 717 amino acids in length, showing a high level of homology to the Echinoderm 77-kDa microtubule-associated protein (EMAP) [3].
• One of these, HuEMAP-1, is identified as the candidate gene for the Usher syndrome 1 a locus (Genomics 43:104-106, 1997) [8].
• Identification of rat EMAP, a delta-glutamate receptor binding protein [9].
• Cell cycle-dependent phosphorylation of the 77 kDa echinoderm microtubule-associated protein (EMAP) in vivo and association with the p34cdc2 kinase [6].
• EMAP localizes to the mitotic spindle as well as the interphase microtubule array and is a likely target for a cell cycle-activated kinase [6].

Anatomical context of EML1

• The echinoderm microtubule-associated protein (EMAP) is a 75-kDa, WD-repeat protein associated with the mitotic spindle apparatus [10].
• An aspartic proteinase previously thought to be unique to erythrocyte membranes, termed "EMAP", has been shown to be closely related to cathepsin E [11].
• These results indicate the location of EMAP on th inner surface of the membranes [12].
• Potential mechanisms for EMAP II's selective effects on endothelial cells have been described [13].

Associations of EML1 with chemical compounds

• As this is not the case with high O2 in an EMA film, the latter can be assumed as a promising configuration for respiring fresh produce, combining the beneficial effect of high O2 and EMAP [14].

Other interactions of EML1

• The nucleotide sequence of a human EMAP-related protein-2 (HuEMAP-2) encodes a protein of 649 amino acids in length [8].

Analytical, diagnostic and therapeutic context of EML1

• Northern blot analysis indicates that HuEMAP-1, and HuEMAP-2 are expressed in different human tissues [8].
• To accomplish this goal, we utilized a low-cost, bubble-column bioreactor to express EMAP as a hexahistidine fusion (6his) protein in baculovirus-infected insect cells [10].
• A polyclonal antisera, raised against SDS-PAGE denatured EMAP, reacted strongly with both MAP-2 and tau on Western blots [15].
• The patterns of immunoelectrophoresis, immunoblotting, and immunoprecipitation of the enzyme were remarkably similar, if not identical, to those of human EMAP [16].
• Cytotoxic lectins (KML-C) were isolated from an extract of Korean mistletoe [Viscum album C. (coloratum)] by affinity chromatography on a hydrolysed Sepharose 4B column, and the chemical and biological properties of KML-C were examined, partly by comparing them with a lectin (EML-1) from European mistletoe[Viscum album L. (loranthaceae)] [17].

References