Disease relevance of Sox15

• Ectopic expression of Sox15 in Rat choriocarcinoma cells enhanced the giant cell differentiation induced by a bHLH transcription factor, Hand1 [1].

High impact information on Sox15

• These results show an increased proportion of the Myf5-positive cells and suggest a role for Sox15 in determining the early myogenic cell lineages during skeletal muscle development [2].
• Histological analysis revealed the normal ultrastructure of myofibers and the presence of comparable amounts of satellite cells in the skeletal muscles of Sox15(-/-) animals compared to wild-type animals [2].
• However, cultured Sox15(-/-) myoblasts displayed a marked delay in differentiation potential in vitro [2].
• Gel mobility shift assays and SELEX analyses showed that Sox15 binds similar DNA sequences as Sox2 but with weaker affinity [3].
• Sox15 is expressed at low levels in several tissues, including testis and muscle [3].

Biological context of Sox15

• This enhancement required both the Hand1-binding region and the transactivation domain of Sox15 [1].
• Sox15 expression was analysed during mouse embryogenesis by whole-mount in situ hybridisation and Real Time RT-PCR [4].
• These results suggest a specific and repressive role for SOX15, requiring the C-terminal domain, during myogenesis [5].
• Electrophoretic mobility shift assays indicated that recombinant SOX15 protein was capable of binding to a consensus DNA binding site for SOX proteins [5].

Anatomical context of Sox15

• Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells [3].
• We also observed that the amount of Sox15 mRNA dramatically increased during the differentiation of mouse trophoblast stem cells [1].
• In situ hybridization analysis of the mouse E8.0 conceptus indicated that Sox15 mRNA was predominantly expressed in the trophoblast giant cells of the placenta [1].
• Binding experiments in cotransfected 293 T cells and in vitro revealed that Sox15 interacted with Hand1 [1].
• Sox15 was found to be expressed in developing mouse gonads from 11.5 dpc to 13.5 dpc with a peak of expression at 12.5 dpc [4].

Regulatory relationships of Sox15

• Sox15 enhances trophoblast giant cell differentiation induced by Hand1 in mouse placenta [1].

Other interactions of Sox15

• In contrast to the early embryonic lethality observed in Sox2-null mice, Sox15-null ES cells and mice were grossly normal [3].
• These results may suggest that the increased transcriptional activity of Hand1 caused by Sox15 might promote the transcription of the target gene resulting in the trophoblast giant cell differentiation in the mouse placenta [1].
• Here, we report the isolation and characterization of mouse Sox15 that was uncovered during a screen for high mobility group box containing transcription factors that are expressed at different levels during skeletal muscle differentiation [5].
• Here, we report that Sox15 transcripts were much more abundant in mouse placenta than in the fetus, the yolk sac, or several adult tissues [1].

References