Disease relevance of Sult1a1

• Hybridization with the Stp probe was more frequent (P less than 0.005) for E coli isolated from calves that died before 2 weeks of age (49 STp-positive isolates of 77 [64%] isolates) than for E coli isolated from calves that died after 2 weeks of age (2 STp-positive of 21 [10%] isolates) [1].
• However, HHV-8 reading frame K1, the positional analogue of Stp/Tip, was found to be significantly variable between different strains [2].

High impact information on Sult1a1

• Six amino acid residues of PST have been chosen for mutagenesis studies on the basis of a model of PST and its sequence alignment with those of available cytosolic and membrane-anchored STs [3].
• In addition to PAPS, phenol sulfotransferase (PST), a member of the ST family, utilizes other nucleotides as substrates with much less catalytic efficiency [Lin, E. S., and Yang, Y. S. (2000) Biochem. Biophys. Res. Commun. 271, 818-822] [3].
• After decay of extant PST in steroid-free medium, hydrocortisone reinduced the expression of PST three to fivefold [4].
• To determine what factors may control PST expression, extracts of serum-free, growth factor-supplemented cultures of bovine bronchial epithelial cells were assayed for PST activity and PST antigen [4].
• The tracheobronchial epithelium is commonly exposed to phenolic drugs and pollutants, and metabolic sulfation and PST activity in this tissue have been previously demonstrated [4].

Biological context of Sult1a1

• Furthermore, we have determined by genotyping of murine interspecific backcross progeny that the homologous gene in mouse (Stp) localizes to the syntenic region of mouse chromosome 7 near the D7Mit8 (at 54 cM) and D7Bir1 markers [5].
• Hydrolysis of arginine into urea and ornithine (Orn) was observed to take place in several segments of the rat nephron including cortical and medullary pars recta of the proximal tubule (PST) and collecting duct (CD) [6].
• Although this procedure revealed collinear organization and extensive homologies with the open reading frames of herpesvirus saimiri, genes with homology to the known oncoproteins (Stp, Tip) were not identified in the HHV-8 genome [2].

Anatomical context of Sult1a1

• Comparison of PCR-based mutation detection methods and application for identification of mouse Sult1a1 mutant embryonic stem cell clones using pooled templates [7].
• Regulation of phenol sulfotransferase expression in cultured bovine bronchial epithelial cells by hydrocortisone [4].

Associations of Sult1a1 with chemical compounds

• The third clone, mSTp1 showed high identity to rSTp, hSTp1, hSTp3, and rSTp1C1, suggesting that mSTp1 belongs to the phenol family [8].
• In both species, as well as in the rat, the PST was the site of the highest urea + Orn production, with an intensity increasing from cortex to medulla [6].

Other interactions of Sult1a1

• Rat phenol-preferring sulfotransferase genes (Stp and Stp2): localization to mouse chromosomes 7 and 17 [9].

Analytical, diagnostic and therapeutic context of Sult1a1

• Sult1a1, 1b1, 1c1, 1c2, 1d1, 1e1, 2a1/2, 2b1, 3a1, 4a1, 5a1, PAPSs1, and PAPSs2 mRNA expression was quantified in 14 tissues from male and female mice using the branched DNA signal amplification assay [10].
• Molecular cloning of cDNA encoding the phenol/aryl form of sulfotransferase (mSTp1) from mouse liver [11].
• Western blots using mouse anti-bovine PST revealed corresponding increases in 32 kDa PST protein levels in response to hydrocortisone [4].

References