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Gene Review

Ube2i  -  ubiquitin-conjugating enzyme E2I

Mus musculus

Synonyms: 5830467E05Rik, F830028O17Rik, Mmubc9, SUMO-conjugating enzyme UBC9, SUMO-protein ligase, ...
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Disease relevance of Ube2i


High impact information on Ube2i

  • When a yeast two-hybrid assay is used, deletion analysis of the interacting domain on Vsx-1 shows that Ubc9 binds to a nuclear localization signal (NLS) at the NH(2) terminus of the homeodomain [2].
  • In SW13 AK1 cells that express low levels of endogenous Ubc9, Vsx-1 accumulates in a perinuclear ring and colocalizes with an endoplasmic reticulum marker [2].
  • Direct involvement of the ubiquitin-conjugating enzyme Ubc9/Hus5 in the degradation of IkappaBalpha [3].
  • A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein [4].
  • Transient transfection assays in COS-1 cells showed that Ubc9 increased MR transactivation of reporter constructs containing MRE, ENaC, or MMTV promoter in a hormone-sensitive manner [5].

Biological context of Ube2i

  • Following siRNA knockdown of IPO13 activity in the fetal ovary, fewer germ cells are found to progress to the late-pachytene stage of meiosis and nuclear accumulation of UBC9 is reduced [6].
  • Loss of Ubc9 leads to major chromosome condensation and segregation defects [7].
  • In culture, Ubc9 mutant blastocysts are viable, but fail to expand after 2 days and show apoptosis of the inner cell mass [7].
  • We investigated the role of the SUMO-1-conjugating enzyme Ubc9 in MR transactivation [5].
  • Failed mitosis in the absence of Ubc9 is not necessarily coupled with cell death [8].

Anatomical context of Ube2i

  • This finding is consistent with the progressive accumulation of UBC9 in the nucleus of the meiotic germ cells after the onset of L-Ipo13 expression [6].
  • Ubc9-deficient cells also show severe defects in nuclear organization, including nuclear envelope dysmorphy and disruption of nucleoli and PML nuclear bodies [7].
  • In sum, our data indicate that DeltaNp63alpha is ubiquitinated in a Nedd4- and sumoylated in a Ubc9-dependent fashion, and that these modifications can regulate DeltaNp63alpha stability in the zebrafish ectoderm [9].
  • Ubc9 expression is essential for myotube formation in C2C12 [10].
  • Interestingly, knockdown of the SUMO-conjugating enzyme, Ubc9, severely compromises C2C12 muscle cell terminal differentiation [10].

Associations of Ube2i with chemical compounds

  • Indeed, chromatin immunoprecipitation assays demonstrated that endogenous MR, Ubc9, and SRC-1 were recruited to an endogenous ENaC gene promoter in a largely aldosterone-dependent manner [5].
  • Ets-1 with Lys-15 mutated to an arginine still binds UBC9 with an affinity similar to the wild type protein, but is no longer sumoylated [11].


  1. mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect. Hateboer, G., Hijmans, E.M., Nooij, J.B., Schlenker, S., Jentsch, S., Bernards, R. J. Biol. Chem. (1996) [Pubmed]
  2. Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification. Kurtzman, A.L., Schechter, N. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  3. Direct involvement of the ubiquitin-conjugating enzyme Ubc9/Hus5 in the degradation of IkappaBalpha. Tashiro, K., Pando, M.P., Kanegae, Y., Wamsley, P.M., Inoue, S., Verma, I.M. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  4. Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes. Kovalenko, O.V., Plug, A.W., Haaf, T., Gonda, D.K., Ashley, T., Ward, D.C., Radding, C.M., Golub, E.I. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  5. Coactivation of the N-terminal transactivation of mineralocorticoid receptor by Ubc9. Yokota, K., Shibata, H., Kurihara, I., Kobayashi, S., Suda, N., Murai-Takeda, A., Saito, I., Kitagawa, H., Kato, S., Saruta, T., Itoh, H. J. Biol. Chem. (2007) [Pubmed]
  6. Stage-specific Importin13 activity influences meiosis of germ cells in the mouse. Yamaguchi, Y.L., Tanaka, S.S., Yasuda, K., Matsui, Y., Tam, P.P. Dev. Biol. (2006) [Pubmed]
  7. The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice. Nacerddine, K., Lehembre, F., Bhaumik, M., Artus, J., Cohen-Tannoudji, M., Babinet, C., Pandolfi, P.P., Dejean, A. Dev. Cell (2005) [Pubmed]
  8. Ubc9 Regulates Mitosis and Cell Survival during Zebrafish Development. Nowak, M., Hammerschmidt, M. Mol. Biol. Cell (2006) [Pubmed]
  9. Destabilization of DeltaNp63alpha by Nedd4-mediated ubiquitination and Ubc9-mediated sumoylation, and its implications on dorsoventral patterning of the zebrafish embryo. Bakkers, J., Camacho-Carvajal, M., Nowak, M., Kramer, C., Danger, B., Hammerschmidt, M. Cell Cycle (2005) [Pubmed]
  10. Ubc9 expression is essential for myotube formation in C2C12. Riquelme, C., Barthel, K.K., Qin, X.F., Liu, X. Exp. Cell Res. (2006) [Pubmed]
  11. Beads-on-a-string, characterization of ETS-1 sumoylated within its flexible N-terminal sequence. Macauley, M.S., Errington, W.J., Schärpf, M., Mackereth, C.D., Blaszczak, A.G., Graves, B.J., McIntosh, L.P. J. Biol. Chem. (2006) [Pubmed]
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