Disease relevance of ROCK2

• Our findings indicate that p160ROCK-mediated Ca2+ sensitization is involved in the pathophysiology of hypertension and suggest that compounds that inhibit this process might be useful therapeutically [1].

High impact information on ROCK2

• We identified the Y-27632 target as a Rho-associated protein kinase, p160ROCK [1].
• Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension [1].
• Shear stress-induced cell alignment and stress fiber formation were inhibited by the dominant-negative mutants of Rho and p160ROCK, but not by the dominant-negative mutant of Cdc42, indicating that the Rho-p160ROCK pathway regulates the cytoskeletal reorganization in response to shear stress [2].
• The Rho-associated protein kinase p160ROCK is required for centrosome positioning [3].
• These observations suggest that nucleus-localized ROCK2 targets p300 for phosphorylation to regulate its acetyltransferase activity [4].

Chemical compound and disease context of ROCK2

• Y-27632 consistently suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells and dramatically corrects hypertension in several hypertensive rat models [1].

Biological context of ROCK2

• PURPOSE: To elucidate the roles of Rho-associated protein kinase (ROCK) in regulating intraocular pressure (IOP) and outflow facility in the rabbit eye [5].

Anatomical context of ROCK2

• While the inhibition of p160ROCK and its ensuing disruption of actin filaments decreased the basal c-fos activity in static ECs (no flow), it did not affect the shear-inductive effect [6].

Analytical, diagnostic and therapeutic context of ROCK2

• Nuclear ROCK2 is present in a large protein complex and partially cofractionates with p300 by gel filtration analysis [4].
• By immunofluorescence, ROCK2 partially colocalizes with p300 in distinct insoluble nuclear structures [4].
• The inhibition of a Rho-associated kinase, p160ROCK, decreased the traction force and migration speed under both static and shear conditions and eliminated the shear-enhancement of migration speed [7].
• Precipitating antibody concentration responses to crude northern fowl mite extract (CME) and bovine serum albumin (BSA) injections were compared in White Rock and Fayoumi hens with two-dimensional immunoelectrophoresis and rocket electrophoresis [8].

References