The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

Rock2  -  Rho-associated coiled-coil containing...

Rattus norvegicus

Synonyms: ROCK-II, ROK, ROKalpha, Rho-associated protein kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on Rock2

 

Biological context of Rock2

 

Anatomical context of Rock2

  • p160 RhoA-binding kinase ROKalpha induces neurite retraction [4].
  • Here we examined the morphological effect of p160 RhoA-binding kinase ROKalpha, a target for RhoA recently identified, on the nerve growth factor-differentiated PC12 cells [4].
  • Cellular redistribution of PKCalpha, rhoA, and ROKalpha following smooth muscle agonist stimulation [5].
  • GTP gamma S (10(-4) m) stimulated the translocation of RhoA and Rock-2 from the cytosol to the membrane, which was sustained for more than 60 min [6].
  • The mRNA expressions of rho A, ROK alpha and ROK beta in the pregnant myometrium were found to increase in comparison to those in the non-pregnant myometrium [7].
 

Associations of Rock2 with chemical compounds

  • However, introduction of kinase-dead MRCKalpha did not result in NGF-independent neurite outgrowth as observed with dominant negative kinase-dead ROKalpha or the Rho inhibitor C3 [3].
  • However, genistein did not block neuronal morphological changes induced by microinjection of a constitutively active mutant of ROKalpha [8].
  • Before treatment with GTP gamma S, RhoA and Rock-2 were detected primarily in the cytosolic fraction [6].
  • Expression of ROKalpha, ROKbeta and RhoA was analysed using western blot, whereas Rho guanine nucleotide exchange factors (RhoGEF) were measured at the mRNA level [9].
 

Regulatory relationships of Rock2

  • These results demonstrate that ROKalpha induces neurite retraction acting downstream of Rho in neuronal cells [4].
 

Other interactions of Rock2

  • Furthermore, we were able to show that binding of these novel binding proteins, as well as the previously described interaction partners ROK alpha (rho A binding kinase alpha) and alpha- and beta-tubulin, bind to specific cytosolic sequences of NCAM [10].
  • These results suggest that although ROKalpha is involved in neurite retraction promoted by RhoA, the related MRCKalpha is conversely involved in neurite outgrowth promoted by Cdc42 and Rac [3].
  • Neurite outgrowth induced by NGF or kinase-dead ROKalpha was inhibited by dominant negative Cdc42(N17), Rac1(N17), and the Src homology 3 domain of c-Crk, indicating the participation of common downstream components [3].
 

Analytical, diagnostic and therapeutic context of Rock2

References

  1. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Leung, T., Chen, X.Q., Manser, E., Lim, L. Mol. Cell. Biol. (1996) [Pubmed]
  2. Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells. Begum, N., Sandu, O.A., Ito, M., Lohmann, S.M., Smolenski, A. J. Biol. Chem. (2002) [Pubmed]
  3. The myotonic dystrophy kinase-related Cdc42-binding kinase is involved in the regulation of neurite outgrowth in PC12 cells. Chen, X.Q., Tan, I., Leung, T., Lim, L. J. Biol. Chem. (1999) [Pubmed]
  4. p160 RhoA-binding kinase ROKalpha induces neurite retraction. Katoh, H., Aoki, J., Ichikawa, A., Negishi, M. J. Biol. Chem. (1998) [Pubmed]
  5. Cellular redistribution of PKCalpha, rhoA, and ROKalpha following smooth muscle agonist stimulation. Taggart, M.J., Lee, Y.H., Morgan, K.G. Exp. Cell Res. (1999) [Pubmed]
  6. Sevoflurane inhibits guanosine 5'-[gamma-thio]triphosphate-stimulated, Rho/Rho-kinase-mediated contraction of isolated rat aortic smooth muscle. Yu, J., Ogawa, K., Tokinaga, Y., Hatano, Y. Anesthesiology (2003) [Pubmed]
  7. Up-regulation of rho A and rho-kinase mRNAs in the rat myometrium during pregnancy. Niiro, N., Nishimura, J., Sakihara, C., Nakano, H., Kanaide, H. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  8. Signal transduction pathway regulating prostaglandin EP3 receptor-induced neurite retraction: requirement for two different tyrosine kinases. Aoki, J., Katoh, H., Yasui, H., Yamaguchi, Y., Nakamura, K., Hasegawa, H., Ichikawa, A., Negishi, M. Biochem. J. (1999) [Pubmed]
  9. Expression and functional role of the RhoA/Rho-kinase pathway in rat coeliac artery. Teixeira, C.E., Jin, L., Ying, Z., Palmer, T., Priviero, F.B., Webb, R.C. Clin. Exp. Pharmacol. Physiol. (2005) [Pubmed]
  10. Novel cytosolic binding partners of the neural cell adhesion molecule: mapping the binding domains of PLC gamma, LANP, TOAD-64, syndapin, PP1, and PP2A. Büttner, B., Kannicht, C., Reutter, W., Horstkorte, R. Biochemistry (2005) [Pubmed]
 
WikiGenes - Universities