Disease relevance of Jag1

• Point mutations in Jagged-1 gene are the cause for Alagille syndrome, associated with paucity of intrahepatic bile ducts [1].

High impact information on Jag1

• Finally, the biological activity of Jagged was tested using a cell culture assay in which Jagged activates rat Notch1 expressed in myoblasts and prevents muscle cell differentiation [2].
• It has been proposed that Jag1 has a role in specifying sensory versus nonsensory epithelium within the ear [Adam, J., Myat, A., Roux, I. L., Eddison, M., Henrique, D., Ish-Horowicz, D. & Lewis, J. (1998) Development (Cambridge, U.K.) 125, 4645--4654] [3].
• The Notch ligand Jagged1 is required for inner ear sensory development [3].
• Both Notch-1 and Jagged-1 proteins were upregulated in hepatocytes after partial hepatectomy up to day 4 [1].
• A decrease of either Notch1 or Jag1 expression by antisense oligonucleotides in cultures of the developing sensory epithelium resulted in an increase in the number of hair cells [4].

Biological context of Jag1

• Gene expressions of Notch-1 and Jagged-1 were detected in cultured BM cells on day 3 [5].
• We also investigated the gene expressions of Notch receptor-1 (Notch-1) and its ligand Jagged-1 in BM cell differentiation [5].
• At lower cell density, Notch1- or Jagged1-expressing cells were not seen [6].
• The downregulation of Jagged-1 and Notch-3 was associated with a decrease in CBF-1-mediated gene transcription activation and a fall in the mRNA levels of the downstream target transcription factor HESR-1 [7].

Anatomical context of Jag1

• Addition of recombinant Jagged-1 protein to primary cultures of hepatocytes stimulated hepatocyte DNA synthesis [1].
• Jagged1 protein enhances the differentiation of mesenchymal stem cells into cardiomyocytes [8].
• Jagged was expressed in distinct regions within the optic vesicle, ciliary body, and lens, with patterns that changed over time [9].
• The differential expression of Notch receptors and their ligands, especially Jagged1, throughout thymus development plays a key role in the generation of the different CD4:CD8 cell ratios [10].
• A low increase of ligand expression was observed near the injury with Delta1 expression along the dentin wall and Jagged1 in the stroma [11].

Regulatory relationships of Jag1

• 3. Jagged1 protein can activate Notch signal and enhance the differentiation of MSC into cardiomyocyte, while the effect can be inhibited by DAPT [8].

Other interactions of Jag1

• This study documents that the Notch-3 receptor, the ligand Jagged-1, and the downstream transcription factor, HESR-1, are expressed in the normal adult rat carotid artery, and that this expression is modulated after vascular injury [7].
• Here, we examined the expression pattern of four Delta, Serrate and Lag-2 (DSL) ligands, Jagged1, Jagged2, Delta-like1 (Dl1) and Delta-like 3 (Dl3), in late embryonic and postnatal rat brain by in situ hybridization [12].

Analytical, diagnostic and therapeutic context of Jag1

• Furthermore, injection of silencing RNA for Notch and Jagged-1 to livers 2 days before partial hepatectomy significantly suppressed proliferation of hepatocytes at days 2 to 4 of the regenerative response [1].
• First, sequence analysis reveals substantial homology between Jagged and invertebrate ligands for the LIN-12/Notch proteins [2].
• Among them, the expression pattern of 1 male-enriched gene, jagged homolog 1, and those of 2 female-enriched genes, p27Kip1 and p130, were confirmed to be consistent with microarray data by RT-PCR [13].

References