High impact information on Xrcc5

• Almost all methylated CpG dinucleotides were demethylated in Ku-80 cells after 5-azacytidine treatment [1].
• Bisulfite genomic sequencing revealed that all 21 CpG dinucleotides in MT-I immediate promoter were methylated in Ku-80 cells, whereas only four CpG dinucleotides were methylated in Rat-1 cells [1].
• We have shown previously that the heavy metal-induced metallothionein-I (MT-I) gene expression is specifically repressed in a rat fibroblast cell line (Ku-80) overexpressing the 80-kDa subunit of Ku autoantigen but not in cell lines overexpressing the 70-kDa subunit or Ku heterodimer [1].
• These findings suggest that there are some defects in maintaining the levels of G22p1 and Xrcc5 proteins in the nuclei of LEC rat cells [2].
• An analysis of fibroblasts from backcross rats showed that the deficiency in nuclear accumulation of G22p1 and Xrcc5 proteins is genetically linked to enhanced radiosensitivity [2].

Biological context of Xrcc5

• DNA-PK is a heterotrimeric protein complex comprised of two components: a large catalytic subunit, Prkdc, with serine/threonine kinase activity and a DNA-targeting component, G22p1 and Xrcc5 [2].
• During DNA damage, DNA repair proteins Ku70 and Ku80 prevent cell death, but severe DNA damage beyond the repair capacity of the DNA repair proteins triggers necrosis or apoptosis [3].
• Moreover, okadaic acid-induced phosphorylation of Ku80 was inhibited by nanomolar concentrations of the protein kinase inhibitor staurosporine [4].

Anatomical context of Xrcc5

• Although these proteins were observed mainly in the nuclei of human fibroblasts, approximately 80% of the intensities of immunofluorescence from both G22p1 and Xrcc5 was observed in the cytoplasm of rat fibroblasts [5].

Associations of Xrcc5 with chemical compounds

• G/GO treatment caused nuclear loss of both Ku70 and Ku80 in Wild cells and pcN-3 cells [3].
• The total triglyceride recovered in 24 h was highest after CLO (410 mg) and lowest with K80 (146 mg) [6].
• An increase in the weight percentage of some characteristic fatty acids of the lipid mixtures was observed: oleic acid for butter, oleic and linoleic acids for CO, EPA and DHA for CLO, MO, and K80 [6].

Other interactions of Xrcc5

• These results suggest that nuclear translocation of G22p1 and Xrcc5 is important for the fast repair process of PLD in rat cells [5].

Analytical, diagnostic and therapeutic context of Xrcc5

• With Western blot analysis, we found no significant difference in Ku80 protein expression between preconditioned and control groups after MI [7].

References