High impact information on ImpE2

• However, males have been shown to produce YPs when treated with the steroid hormone 20-hydroxyecdysone (20-HE) [1].
• An apical surface glycoprotein, designated gp125 for its apparent molecular weight of 125,000, appears in Ca2(+)-free, ionic detergent extracts of imaginal discs of Drosophila melanogaster in response to the steroid hormone, 20-hydroxyecdysone (20-HE) [2].
• The pupal cuticle is formed in vitro by imaginal discs in response to a 6 h pulse of 20-hydroxyecdysone (20-HE) [3].
• Culture of fourth instar larval epidermis with 4 microM 20-hydroxyecdysone (2 micrograms/ml 20HE) caused the appearance of MHR3 mRNA within 3 hr and maximal expression by 6 hr; after 12 hr continuous exposure to 20HE, the mRNA level declined [4].
• We have investigated the regulatory interactions between 20HE, BRC, and a set of genes expressed in the fat body of third-instar Drosophila larvae [5].

Biological context of ImpE2

• Although the temporal and spatial expression of the IMP-E2 gene are consistent with a role in disc morphogenesis, its specific functions remain to be determined [6].
• When Day 2 fourth instar epidermis was explanted before the molting ecdysteroid rise and cultured with 1-3 micrograms/ml 20-HE for 17 hr and then for 24 hr in hormone-free medium, DDC expression was three- to fourfold higher than that in epidermis cultured in the absence of hormone [7].
• The steroid hormone 20-hydroxyecdysone (20HE) and the Broad-Complex locus (BRC) are involved in regulating developmental changes in gene expression around the time of metamorphosis in Drosophila [5].
• The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed [8].
• The 20-HE induced beta tubulin comigrates with the beta 3 subunit and like the latter, is specific to the mid period of embryogenesis [9].

Anatomical context of ImpE2

• The IMP-E2 product is secreted at the apical surface of the disc epithelium in association with disc morphogenesis [6].
• Two Drosophila imaginal disc cell lines, C18+ (sensitive to 20-hydroxyecdysone, 20HE) and C18R (resistant to 20HE) were exposed to the ecdysteroid agonists RH5849 and RH5992 and the ecdysteroids inokosterone, makisterone A and muristerone A [10].
• Depression of synaptic efficacy in high- and low-output Drosophila neuromuscular junctions by the molting hormone (20-HE) [11].
• The results showed that the high-output (Is) and the low-output (Ib) motor nerve terminals, which innervate muscles 6 and 7, released fewer synaptic vesicles for each stimulation while exposed to 20-HE [11].
• Pigmentation in the nonphotoreceptor pigment cells in cultures from the prepupa required the presence of 20-hydroxyecdysone (20-HE) (1 micrograms/ml), whereas neurite outgrowth was seen in the absence of 20-HE [12].

Associations of ImpE2 with chemical compounds

• The steroid hormone 20-hydroxyecdysone (20-HE) induces imaginal discs to form adult appendages in Drosophila [13].

References