High impact information on Pcp

• A pupal cuticle protein gene has been found within an intron of a Drosophila gene that encodes three purine pathway enzymatic activities [1].
• The low molecular weight pupal cuticle proteins accumulate in response to a pulse of the insect steroid hormone 20-hydroxyecdysone and begin to appear 6 h after the withdrawal of the hormone from the culture medium [2].
• A set of low molecular weight (15,000-25,000) pupal cuticle proteins are synthesized by the imaginal disk epithelium before pupation [2].
• A putative pupal cuticle gene, EDG84A, is expressed slightly following FTZ-F1 expression during the prepupal period and carries a strong FTZ-F1 binding site between bases 100 and 92 upstream of its transcription start site [3].
• The pupal cuticle is formed in vitro by imaginal discs in response to a 6 h pulse of 20-hydroxyecdysone (20-HE) [4].

Biological context of Pcp

• The excision repair of UV-induced pyrimidine dimers was investigated in three genes: Gart, Notch and white in a permanent Drosophila cell line Kc, derived from wild type Drosophila melanogaster embryonic cells [5].
• Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster [6].
• We have studied possible phylogenetic relationships among these five genes by comparing nucleotide sequences of four LCP genes with that of the PCP gene [7].
• We have detected connections between the epithelium and pupal cuticle that are presumably targets of chitinase and are necessary for maintaining normal tissue shape during morphogenesis [8].

Anatomical context of Pcp

• In this cell line Gart and Notch are actively transcribed, whereas white is not expressed [5].
• We conclude that a third EDG encodes a cuticle protein because the conceptual glycine-rich protein contains sequence motifs similar to those found in insect egg shell proteins and vertebrate cytokeratins and because expression of this gene is limited to tissues that deposit the pupal cuticle [9].

Associations of Pcp with chemical compounds

• Whereas the purine pathway gene is active throughout development, the intronic cuticle protein gene is expressed primarily over a 3 hr period in the abdominal epidermal cells of prepupae that secrete the pupal cuticle [1].
• We summarize the regulation by JH of the Broad transcription factor that normally appears in the epidermis in the final larval instar and specifies pupal cuticle formation at the metamorphic molt [10].
• Repair of UV-induced pyrimidine dimers in the individual genes Gart, Notch and white from Drosophila melanogaster cell lines [5].

Other interactions of Pcp

• BetaFTZ-F1 dependent and independent activation of Edg78E, a pupal cuticle gene, during the early metamorphic period in Drosophila melanogaster [11].

References