The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

umuC  -  UV mutagenesis and repair protein;...

Escherichia coli

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of umuC


High impact information on umuC

  • Along with the sequence homologies previously found within the UmuC-like protein family, these results indicate that the uncovered DNA polymerase activity may be a common feature of all these homologous proteins [6].
  • One component of the SOS response is a marked increase in mutation rate, dependent on RecA protein and the induced mutagenesis proteins UmuC and UmuD [7].
  • This enhanced mutation rate requires the action of the UmuD and UmuC proteins, which are induced as part of the SOS response to DNA damage [8].
  • Mutational analysis of the corresponding motif in UmuC showed that it is dispensable for induced mutagenesis, but that alterations in this motif result in loss of the cold-sensitive phenotype [9].
  • First, UmuD and UmuC together participate in a cell cycle checkpoint control; second, UmuD'(2)C enables translesion DNA replication over any remaining unrepaired or irreparable lesions in the DNA [10].

Chemical compound and disease context of umuC

  • These results suggest that genistein may interfere with expression of the SOS response, including the UmuC-mediated lesion bypass mechanism that is necessary for UV-mutagenesis and the generation of transversions by ENU in E. coli [11].
  • UmuC product contributes to the inhibition of dimer excision produced by thymine-less-amino acid-less pretreatment in UV-irradiated Escherichia coli [12].

Biological context of umuC


Associations of umuC with chemical compounds

  • A multicopy plasmid that expressed UmuC at physiological levels was constructed and randomly mutagenized in vitro by exposure to hydroxylamine [17].
  • Despite these observations, mutagenesis by nitracrine appears to be independent of the UmuC gene product, and hence nitracrine differs from most (but not all) other chemicals which generate mutations via the SOS response [18].
  • Data suggest that the UmuC gene product is not absolutely necessary for the inhibition of dimer excision in UV-irradiated thymine-less-amino acid-less pretreated cells, but that it contributes to it [12].
  • Recently, we discovered that the intracellular levels of the UmuD and UmuC proteins are kept to a minimum by the Lon serine protease [19].


  1. Lon-mediated proteolysis of the Escherichia coli UmuD mutagenesis protein: in vitro degradation and identification of residues required for proteolysis. Gonzalez, M., Frank, E.G., Levine, A.S., Woodgate, R. Genes Dev. (1998) [Pubmed]
  2. Plasmid-encoded MucB protein is a DNA polymerase (pol RI) specialized for lesion bypass in the presence of MucA', RecA, and SSB. Goldsmith, M., Sarov-Blat, L., Livneh, Z. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  3. UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli. Tessman, I. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  4. Roles of YqjH and YqjW, homologs of the Escherichia coli UmuC/DinB or Y superfamily of DNA polymerases, in stationary-phase mutagenesis and UV-induced mutagenesis of Bacillus subtilis. Sung, H.M., Yeamans, G., Ross, C.A., Yasbin, R.E. J. Bacteriol. (2003) [Pubmed]
  5. Identification of a DinB/UmuC homolog in the archeon Sulfolobus solfataricus. Kulaeva, O.I., Koonin, E.V., McDonald, J.P., Randall, S.K., Rabinovich, N., Connaughton, J.F., Levine, A.S., Woodgate, R. Mutat. Res. (1996) [Pubmed]
  6. The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis. Wagner, J., Gruz, P., Kim, S.R., Yamada, M., Matsui, K., Fuchs, R.P., Nohmi, T. Mol. Cell (1999) [Pubmed]
  7. Activity of the purified mutagenesis proteins UmuC, UmuD', and RecA in replicative bypass of an abasic DNA lesion by DNA polymerase III. Rajagopalan, M., Lu, C., Woodgate, R., O'Donnell, M., Goodman, M.F., Echols, H. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  8. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA. Burckhardt, S.E., Woodgate, R., Scheuermann, R.H., Echols, H. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  9. Two processivity clamp interactions differentially alter the dual activities of UmuC. Beuning, P.J., Sawicka, D., Barsky, D., Walker, G.C. Mol. Microbiol. (2006) [Pubmed]
  10. umuDC-mediated cold sensitivity is a manifestation of functions of the UmuD(2)C complex involved in a DNA damage checkpoint control. Sutton, M.D., Walker, G.C. J. Bacteriol. (2001) [Pubmed]
  11. Reduction of ENU-induced transversion mutations by the isoflavone genistein in Escherichia coli. Yang, Y., Fix, D. Mutat. Res. (2001) [Pubmed]
  12. UmuC product contributes to the inhibition of dimer excision produced by thymine-less-amino acid-less pretreatment in UV-irradiated Escherichia coli. Masek, F., Sedliaková, M. J. Photochem. Photobiol. B, Biol. (1993) [Pubmed]
  13. Genetic analysis of the anti-mutagenic effect of genistein in Escherichia coli. Yang, Y., Fix, D. Mutat. Res. (2006) [Pubmed]
  14. dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues. Ohmori, H., Hatada, E., Qiao, Y., Tsuji, M., Fukuda, R. Mutat. Res. (1995) [Pubmed]
  15. Identification of a mutation causing increased expression of the tas gene in Escherichia coli FX-11. Johnson, J.M., Ding, W., Henkhaus, J., Fix, D. Mutat. Res. (2001) [Pubmed]
  16. Identification of mucAB-like homologs on two IncT plasmids, R394 and Rts-1. Koch, W.H., Fernández de Henestrosa, A.R., Woodgate, R. Mutat. Res. (2000) [Pubmed]
  17. Isolation and characterization of novel plasmid-encoded umuC mutants. Woodgate, R., Singh, M., Kulaeva, O.I., Frank, E.G., Levine, A.S., Koch, W.H. J. Bacteriol. (1994) [Pubmed]
  18. Mutagenesis by the anti-tumour drug nitracrine in Escherichia coli. MacPhee, D.G., Liaskou, D. Mutat. Res. (1989) [Pubmed]
  19. Structural insights into the regulation of SOS mutagenesis. Gonzalez, M., Frank, E.G., McDonald, J.P., Levine, A.S., Woodgate, R. Acta Biochim. Pol. (1998) [Pubmed]
WikiGenes - Universities