Disease relevance of LOC422652

• Application of the proposed method is shown for alfa-fetoprotein (AFP) and endogenous provirus (ev-3) RNA detection in rat and chicken embryos, respectively [1].
• Morphologically well-differentiated neurons (ganglion cells) appeared positively labeled for AFP, contrasting with weakly stained other cell types: these included spindle-shaped cells identified as Schwann cells and larger, often closely packed cells, which could be less mature neurons [2].

High impact information on LOC422652

• The alpha1-fetoprotein (AFP) gene is located between the albumin and alpha-albumin genes and is activated by transcription factor FTF (fetoprotein transcription factor), presumed to transduce early developmental signals to the albumin gene cluster [3].
• Transcription of the oncodevelopmental protein, alpha-fetoprotein (AFP), is stimulated by retinoic acid (RA) in neoplastic cells [4].
• To study RA regulation of AFP gene expression, the 5'-flanking region of AFP gene was cloned and analyzed [4].
• In the present study, transfection of deletion mutants and sequence analysis revealed a retinoid X receptor response element (AFP-RXRE) located at position -139 to -127 of the AFP promoter [4].
• At 7 days of embryonic age, all the plasma AFP species are present in cerebrospinal fluid [5].

Biological context of LOC422652

• These results confirm the occurrence of such a significant carrier globulin to embryonic development in one more tissue that can be added to the short list of AFP-producing tissues [6].
• The results presented here strongly suggest a close association of AFP with cell proliferation and differentiation [7].

Anatomical context of LOC422652

• These results are discussed in relation to mammalian alpha-fetoprotein, and the merits of the chick embryo for studies on the biological function of AFP are considered [8].
• It is concluded that at this time, the uptake of AFP by spinal ganglia cells is switched-off while SA is still taken up [9].
• The aim of this work was to confirm the occurrence of AFP in the chorioallantoic membrane (CAM) from 14-day chick embryo [6].
• Henceforth, the labeling for AFP starts decreasing in the spinal ganglia but not in the trigeminal ganglion [9].
• In order to avoid the influence of the choroid plexuses in the transport, via the cerebrospinal fluid, of these proteins to brain cells, we studied the presence of AFP and SA within chicken sensory ganglion cells during ontogeny using the indirect immunoperoxidase technique [9].

Associations of LOC422652 with chemical compounds

• The ontogeny of alpha-fetoprotein (AFP) has been studied in the chicken (from 7 days of incubation until 2 days after hatching) using (1) the two-dimensional immunoelectrophoresis technique, (2) the polyacrylamide gel electrophoresis, and (3) the high resolution two-dimensional polyacrylamide gel electrophoresis [5].
• In 7-day-old chick embryo, AFP carries 16% and fSA 6% free FAs, the rest being carried as phospholipids, which differs from rat and pig AFP, where all fatty acids are carried like free fatty acids [10].

Analytical, diagnostic and therapeutic context of LOC422652

• AFP had previously been detected by immunoelectrophoresis in CAM extracts under the suspicion that it could be a mere artifact resulting from blood contamination [6].
• The joint use of organ culture and immunoperoxidase techniques has enabled us to find evidence for the synthesis of AFP in the cells of this layer [6].
• Chicken alpha-fetoprotein (AFP) from the plasma of 12-day-old chick embryos was purified by electroelution from SDS/PAGE gels, and used to produce polyclonal and monoclonal antibodies [11].

References