The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

PFKFB1  -  6-phosphofructo-2-kinase/fructose-2,6...

Homo sapiens

Synonyms: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, 6PF-2-K/Fru-2,6-P2ase liver isozyme, F6PK, HL2K, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of PFKFB1


High impact information on PFKFB1

  • The structure at 2.1 A resolution shows that the overall folding and functional dimerization are very similar to those of the liver (PFKFB1) and testis (PFKFB4) forms, as expected from sequence homology [3].
  • Although none of these mutations affected the interaction of GCK with PFKFB1, we found that the R308W mutation caused protein instability and increased the strength of interaction with GCKR [4].
  • Our findings demonstrate involvement of SREBP-1a in the transcriptional activation of PFKFB1, and we conclude that SREBP-1a may exert a key role mediating postprandial activation of PFKFB1 transcription [5].
  • Transient transfection of HepG2 cells with deleted gene promoter constructs and electrophoretic mobility shift assays allowed us to identify a sterol regulatory element (SRE) to which SRE binding protein-1a (SREBP-1a) binds and transactivates PFKFB1 gene transcription [5].
  • 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key modulator of glycolysis-gluconeogenesis [5].

Biological context of PFKFB1


Anatomical context of PFKFB1

  • A significant negative correlation of the activity of F6PK with age is observed in brain cortex and putamen [7].

Associations of PFKFB1 with chemical compounds

  • Two protein systems, the extracellular soluble domain of the human growth hormone receptor (hGHbp) and the phosphatase domain of PFKFB1 (BPase), were used for the study on effects of DMSO on protein stability, protein aggregation, and binding of drug compounds [8].

Other interactions of PFKFB1

  • PFKFB3 (iPFK2) is one of four tissue-specific PFKFB isozymes that have been identified to date [9].

Analytical, diagnostic and therapeutic context of PFKFB1


  1. Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFKFB genes in gastric cancers. Bobarykina, A.Y., Minchenko, D.O., Opentanova, I.L., Moenner, M., Caro, J., Esumi, H., Minchenko, O.H. Acta Biochim. Pol. (2006) [Pubmed]
  2. Expression and hypoxia-responsiveness of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 in mammary gland malignant cell lines. Minchenko, O.H., Opentanova, I.L., Ogura, T., Minchenko, D.O., Komisarenko, S.V., Caro, J., Esumi, H. Acta Biochim. Pol. (2005) [Pubmed]
  3. Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy. Kim, S.G., Manes, N.P., El-Maghrabi, M.R., Lee, Y.H. J. Biol. Chem. (2006) [Pubmed]
  4. Functional analysis of human glucokinase gene mutations causing MODY2: exploring the regulatory mechanisms of glucokinase activity. Garc??a-Herrero, C.M., Gal??n, M., Vincent, O., Fl??ndez, B., Gargallo, M., Delgado-Alvarez, E., Bl??zquez, E., Navas, M.A. Diabetologia (2007) [Pubmed]
  5. Sterol regulatory element binding protein-1a transactivates 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene promoter. Metón, I., Egea, M., Anemaet, I.G., Fernández, F., Baanante, I.V. Endocrinology (2006) [Pubmed]
  6. Overexpression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 in the human breast and colon malignant tumors. Minchenko, O.H., Ochiai, A., Opentanova, I.L., Ogura, T., Minchenko, D.O., Caro, J., Komisarenko, S.V., Esumi, H. Biochimie (2005) [Pubmed]
  7. Neurochemical findings in the aging brain. Meier-Ruge, W., Iwangoff, P., Reichlmeier, K., Sandoz, P. Adv. Biochem. Psychopharmacol. (1980) [Pubmed]
  8. DMSO-related effects in protein characterization. Tjernberg, A., Markova, N., Griffiths, W.J., Hallén, D. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening. (2006) [Pubmed]
  9. Cloning and chromosomal characterization of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 gene (PFKFB3, iPFK2). Mahlknecht, U., Chesney, J., Hoelzer, D., Bucala, R. Int. J. Oncol. (2003) [Pubmed]
  10. Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. Hilliker, C.E., Darville, M.I., Aly, M.S., Chikri, M., Szpirer, C., Marynen, P., Rousseau, G.G., Cassiman, J.J. Genomics (1991) [Pubmed]
WikiGenes - Universities