High impact information on MGC138959

• The effects of Ca(2+) binding on the side-chain methyl dynamics of calbindin D(9k) have been characterized by (2)H NMR relaxation rate measurements [1].
• Calbindin D(9k) is a dicalcium binding protein consisting of two helix-loop-helix EF-hand motifs joined together by a flexible linker region where one metal ion can bind to each of the two loops [2].
• Two calbindin D(9k) mutants, (Q22E+P43M) and (Q22N+P43M), were constructed to examine the interaction between Gln 22 and the water molecule in the C-terminal calcium binding site II [3].
• In 1,25(OH)2D3 treated cells, calbindin D-28K was enhanced in cytosol and was detected within the nucleus [4].
• Here we describe the measurement and modeling of pK(a) values for all aspartic and glutamic acid residues in apo calbindin D(9k), a representative of calmodulin-related proteins [5].

Biological context of MGC138959

• Symmetrical stabilization of bound Ca2+ ions in a cooperative pair of EF-hands through hydrogen bonding of coordinating water molecules in calbindin D(9k) [3].
• Kinetics of calcium binding to calbindin mutants [6].
• Modulation and phosphorylation of calbindin-D28K correlates with protein kinase C activation1 [7].
• These studies offer the first report that calbindin-D28K is a phosphoprotein and implicate the PKC signal transduction pathway in its regulation [7].

Anatomical context of MGC138959

• Identification and primary structure of a calbindin 9K binding domain in the plasma membrane Ca2+ pump [8].

Associations of MGC138959 with chemical compounds

• The ionization state of seven glutamate residues, one aspartate, and the C-terminal alpha-COOH group in bovine apo calbindin D(9k) has been studied by measurement and modeling of the pH titration curves and apparent pK(a) values [9].
• According to X-ray crystallographic data the OH group of the single tyrosine of calbindin (Tyr-13) is hydrogen-bonded to the carboxyl group of Glu-35, thus linking the two alpha helices flanking the N-terminal Ca2+ site [10].
• We previously reported that 24 h treatment with 1 alpha, 25-dihydroxyvitamin D3 (1,25-D3), the active form of vitamin D3, induces calbindin-D28K and activates protein kinase C (PKC) in MDBK (Madin-Darby bovine kidney) cells [7].

Other interactions of MGC138959

• The expression of calbindin-D9K (CaBP9K) and calbindin-D28K (CaBP28K) genes in the reproductive system is well established for rodent and avian species, but not for domestic livestock [11].
• The enhancement of calbindin-D28K expression is preceded by activation and translocation of PKC alpha [7].

Analytical, diagnostic and therapeutic context of MGC138959

• Immunofluorescence studies indicated that in control MDBK cells, the majority of calbindin D-28K was localized in cytosol, with a definite concentration in the peri-nuclear region [4].
• Focusing of the electrostatic potential at EF-hands of calbindin D(9k): titration of acidic residues [5].
• In MDBK cells, the calbindin-D28K antibody immunoprecipitates a 28 kDa protein for which phosphorylation is enhanced after treatment for 1 h with TPA or 24 h with 1,25-D3 [7].

References