Disease relevance of BTN1A1

• Ligand specificity and affinity of BT-R1, the Bacillus thuringiensis Cry1A toxin receptor from Manduca sexta, expressed in mammalian and insect cell cultures [1].

High impact information on BTN1A1

• Furthermore, adoptive transfer of BTN-specific T cells prior to active immunization with MOG resulted in a transitory reduction of the clinical symptoms [2].
• The cluster of BTN genes in the extended major histocompatibility complex [3].
• We sequenced the 170-kb cluster of BTN genes in the extended major histocompatibility complex region, 4 Mb telomeric of human leukocyte antigen class I genes, at 6p22 [3].
• Transfection of cultured Spodoptera frugiperda cells with the BT-R1 cDNA imparts Cry1A-specific high-affinity binding activity typical of membranes prepared from larval M. sexta midguts [1].
• BT-R1 therefore remains the only M. sexta Cry1A binding protein to be purified, cloned, and functionally expressed in heterologous cell culture, and for the first time, we are able to correlate the Cry1Aa, Cry1Ab, and Cry1Ac toxin sensitivities of M. sexta to the identity and ligand binding characteristics of a single midgut receptor molecule [1].

Biological context of BTN1A1

• Since milk composition changes throughout lactation time, 2-DE maps in the pH range 4-7 of colostral MFGM and mature MFGM were compared, showing only slight differences in BTN spot distribution [4].
• The bovine butyrophilin encoding gene (BTN) maps to chromosome 23 [5].

Anatomical context of BTN1A1

• A retrospective study, the patients were grouped into: Group 1 neoplastic (n = 55) subdivided into: primary tracheal malignancy (PTM, 16), secondary tracheal malignant (STM, 38), benign tracheal neoplasia (BTN, 1); 21 patients in this group had tracheal patch grafts made of Marlex mesh and pericardium; six had a bifurcation resection [6].

Associations of BTN1A1 with chemical compounds

• There were differences before and after treatment only in bioavailable T (BT), with none in the levels of total T (TT) [7].
• There was a strong correlation before and after treatment in the levels of luteinizing hormone and follicle-stimulating hormone, and a weak negative correlation between gonadotrophins and BT [7].
• The four groups were saline-instilled with the control diet (BCD), bleomycin-instilled with the diet containing taurine and niacin (BTN), and saline-instilled with the diet containing taurine and niacin (STN) [8].

Other interactions of BTN1A1

• In the BT3 subfamily, only BT3.3 has the structural organization of BT [9].
• Among 70 spots, analyzed and identified by MALDI-MS, 13 spots were identified as BTN spots and only one as a fragment of BTN2A1 [4].

Analytical, diagnostic and therapeutic context of BTN1A1

• Using immunofluorescence, we showed surface expression of BTN-green fluorescent protein fusions in mammalian cell transfectants [3].
• Competition assays with BT-R1 prepared from larval M. sexta midguts and transiently expressed in cell culture reveal virtually identical affinities for the Cry1Aa, Cry1Ab, and Cry1Ac toxins, clearly demonstrating the absolute specificity of the receptor for toxins of the lepidopteran-specific Cry1A family [1].

References