Disease relevance of WNT3

• WNT3 and WNT3A mRNAs were also co-expressed in a breast cancer cell line MCF-7, and were down-regulated together by beta-estradiol in MCF-7 cells [1].
• WNT3 was significantly up-regulated in a case of primary breast cancer and in a case of primary rectal cancer among various types of human primary cancers [2].
• WNT3 mRNA was relatively highly expressed in A549 cells (lung cancer) and MKN45 cells (gastric cancer) among 37 human cancer cell lines [2].
• In contrast, in four endometrial carcinoma cell lines (RL95-2, HEC-1-A, AN3 CA and Ishikawa), Wnt2 and Wnt3 mRNAs were absent [3].
• CONCLUSION: The NRAMP1 genetic polymorphisms, especially INT4 and 3'UTR, were closely related to tuberculous pleurisy [4].

High impact information on WNT3

• Wnt3-/- mice develop a normal egg cylinder but do not form a primitive streak, mesoderm or node [5].
• We identified a homozygous nonsense mutation (Q83X) in the WNT3 gene in affected fetuses of the family [6].
• Immunoprecipitation studies demonstrated physical interactions between human Ror2 and mammalian Wnt1 and Wnt3 [7].
• These data, together with the CLP lip phenotype in WNT3(-/-) humans and Wnt9b(-/-) mutant mice, indicate that Wnt3 and Wnt9b signal through the canonical Wnt signaling pathway to regulate midfacial development and lip fusion [8].
• Immunohistochemistry disclosed that WNT3 expression and nuclear localization of beta-catenin were predominant in carcinoma cells at the invasive front [9].

Biological context of WNT3

• WNT14B gene is clustered with WNT3 gene in human chromosome 17q21, and mRNA expression of WNT14B is significantly up-regulated by retinoic acid during the early phase of neuronal differentiation in human NT2 cells [10].
• Here, biological significance of WNT3-WNT14B/WNT15 gene cluster (human chromosome 17q21) and WNT3A-WNT14 gene cluster (human chromosome 1q42) will be reviewed [11].
• WNT3 encoded 355-amino-acid polypeptide with the N-terminal signal peptide and two N-linked glycosylation sites [2].
• The WNT3 gene on the human chromosome 17q21 region consisted of five exons [2].
• The CA repeat marker D17S791 is located within 40 kb of the WNT3 gene on chromosome 17q [12].

Anatomical context of WNT3

• Here, regulation of WNT3 and WNT3A mRNAs in human cancer cell lines was investigated [1].
• WNT3 mRNAs were detected in fetal brain, adult brain, and testis by Northern blot analyses [2].
• RESULTS: The hTERT-stromal and primary stromal cells expressed Wnt5A, while hTERT-MSCs and primary MSCs expressed Wnt3 and Wnt5A [13].

Other interactions of WNT3

• More important, a specific Wnt3-FZD7 interaction was observed by co-immunoprecipitation experiments, which suggest that the action of Wnt3 was mediated via FZD7 [14].

Analytical, diagnostic and therapeutic context of WNT3

• Molecular cloning and characterization of human WNT3 [2].

References