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GP ALPHA 1  -  guanine nucleotide-binding protein alpha-1...

Arabidopsis thaliana

Synonyms: ARABIDOPSIS THALIANA G PROTEIN ALPHA SUBUNIT 1, ATGPA1, G PROTEIN ALPHA SUBUNIT, G PROTEIN ALPHA SUBUNIT 1, G protein alpha subunit 1, ...
 
 
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Disease relevance of GPA1

 

High impact information on GPA1

  • Surprisingly, S1P regulates stomatal apertures and guard cell ion channel activities in wild-type plants, but not in knockout lines of the sole prototypical heterotrimeric G-protein alpha-subunit gene, GPA1 (refs 5, 6, 7-8) [2].
  • We found that Arabidopsis thaliana plants harboring transferred DNA insertional mutations in the sole prototypical heterotrimeric GTP-binding (G) protein alpha subunit gene, GPA1, lack both ABA inhibition of guard cell inward K(+) channels and pH-independent ABA activation of anion channels [3].
  • Results from loss of function and ectopic expression and activation of GPA1 indicate that this subunit is a positive modulator of cell division in plants [4].
  • Inducible overexpression of GPA1 in Arabidopsis confers inducible ectopic cell division [4].
  • The alpha subunit of a prototypical heterotrimeric GTP-binding protein (G protein), which is encoded by a single gene (GPA1) in Arabidopsis, is a modulator of plant cell proliferation. gpa1 null mutants have reduced cell division in aerial tissues throughout development [4].
 

Biological context of GPA1

 

Anatomical context of GPA1

 

Associations of GPA1 with chemical compounds

 

Physical interactions of GPA1

  • However, whether GCR1 actually interacts with GPA1 was unknown [10].
  • One of the identified GPA1-interacting proteins is the cupin-domain protein AtPirin1 [11].
 

Other interactions of GPA1

  • We demonstrate by in vitro pull-down assays, by yeast split-ubiquitin assays, and by coimmunoprecipitation from plant tissue that GCR1 and GPA1 are indeed physically coupled [10].
  • These data indicate that AtPirin1 likely functions immediately downstream of GPA1 in regulating seed germination and early seedling development [11].
  • Single- and double-null mutants for the GPA1, AGB1 genes encoding the alpha and beta subunit of the heterotrimeric G protein, respectively, have wild-type sensitivity to R and FR [12].
  • The amino acid sequence of RGA1 was 77% and 86% identical to the sequences of alpha subunits from Arabidopsis thaliana and tomato (products of GPA1 and TGA1), respectively, and 42% to 69% identical to sequences of mammalian alpha subunits [13].
  • Using seeds that carry a null mutation in the genes encoding RGS protein (AtRGS1) and the alpha-subunit (AtGPA1) of the G protein in Arabidopsis (named rgs1-2 and gpa1-3, respectively), our genetic evidence proved the involvement of the AtRGS1 protein in the modulation of seed germination [14].
 

Analytical, diagnostic and therapeutic context of GPA1

References

  1. Biochemical characteristics of a rice (Oryza sativa L., IR36) G-protein alpha-subunit expressed in Escherichia coli. Seo, H.S., Choi, C.H., Lee, S.Y., Cho, M.J., Bahk, J.D. Biochem. J. (1997) [Pubmed]
  2. Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins. Coursol, S., Fan, L.M., Le Stunff, H., Spiegel, S., Gilroy, S., Assmann, S.M. Nature (2003) [Pubmed]
  3. G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells. Wang, X.Q., Ullah, H., Jones, A.M., Assmann, S.M. Science (2001) [Pubmed]
  4. Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis. Ullah, H., Chen, J.G., Young, J.C., Im, K.H., Sussman, M.R., Jones, A.M. Science (2001) [Pubmed]
  5. The Plastid Protein THYLAKOID FORMATION1 and the Plasma Membrane G-Protein GPA1 Interact in a Novel Sugar-Signaling Mechanism in Arabidopsis. Huang, J., Taylor, J.P., Chen, J.G., Uhrig, J.F., Schnell, D.J., Nakagawa, T., Korth, K.L., Jones, A.M. Plant Cell (2006) [Pubmed]
  6. Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana. Ma, H., Yanofsky, M.F., Meyerowitz, E.M. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  7. G-protein-coupled receptor 1, G-protein Galpha-subunit 1, and prephenate dehydratase 1 are required for blue light-induced production of phenylalanine in etiolated Arabidopsis. Warpeha, K.M., Lateef, S.S., Lapik, Y., Anderson, M., Lee, B.S., Kaufman, L.S. Plant Physiol. (2006) [Pubmed]
  8. Isolation of a putative receptor from Zea mays microsomal membranes that interacts with the G-protein, GP alpha 1. Wise, A., Thomas, P.G., White, I.R., Millner, P.A. FEBS Lett. (1994) [Pubmed]
  9. G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development. Pandey, S., Chen, J.G., Jones, A.M., Assmann, S.M. Plant Physiol. (2006) [Pubmed]
  10. The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha subunit GPA1 and regulates abscisic acid signaling. Pandey, S., Assmann, S.M. Plant Cell (2004) [Pubmed]
  11. The Arabidopsis cupin domain protein AtPirin1 interacts with the G protein alpha-subunit GPA1 and regulates seed germination and early seedling development. Lapik, Y.R., Kaufman, L.S. Plant Cell (2003) [Pubmed]
  12. A reevaluation of the role of the heterotrimeric G protein in coupling light responses in Arabidopsis. Jones, A.M., Ecker, J.R., Chen, J.G. Plant Physiol. (2003) [Pubmed]
  13. Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice. Ishikawa, A., Tsubouchi, H., Iwasaki, Y., Asahi, T. Plant Cell Physiol. (1995) [Pubmed]
  14. The regulator of G-protein signaling proteins involved in sugar and abscisic acid signaling in Arabidopsis seed germination. Chen, Y., Ji, F., Xie, H., Liang, J., Zhang, J. Plant Physiol. (2006) [Pubmed]
 
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