High impact information on Gda

• Rat brain guanine deaminase; correlation with regional levels of cyclic GMP phosphodiesterase [1].
• Correlation analysis of Baker's studies on enzyme inhibition. 1. Guanine deaminase, xanthine oxidase, dihydrofolate reductase, and complement [2].
• A functional histochemical assay that demonstrates the site of guanine deamination shows guanine deaminase activity in a pattern that matched the immunohistochemical localization [3].
• A histochemical assay was used to localize guanine deaminase activity to the dendrites and axons of neurons expressing guanine deaminase [3].
• The expression of guanine deaminase is primarily restricted to forebrain neurons [3].

Anatomical context of Gda

• Depletion of succinic dehydrogenase and guanine deaminase occurred in the cerebrum [4].
• Rat brain microsomes, when they are suspended in moderate ionic strength medium, released enzyme activities of lactate dehydrogenase (LDH, E.C.1.1.1.27), malate dehydrogenase (MDH, E.C.1.1.1.37), adenosine deaminase (ADA, E.C.3.5.4.4), guanine deaminase (GAH, E.C.3.5.4.3), and purine nucleoside phosphorylase (PNP, E.C.2.1.2.4) [5].
• It has been proposed that the selective action of thioguanine and 8-azaguinine on certain tissue is due to lack of guanine deaminase in these susceptible cell lines [6].

Associations of Gda with chemical compounds

• 1. Guanine deaminase [EC 3.5.4.3] was purified to a homogeneous state from rat brain by a procedure involving ammonium sulfate fractionation, DE-52 column chromatography, hydroxylapatite column chromatography, gel filtration on ACA-34 and isoelectric focusing [7].
• Adenosine deaminase (ADA, E.C.3.5.4.4) activity was released in higher proportion than guanine deaminase, purine nucleoside phosphorylase (PNP, E.C.2.1.2.4), 5'-nucleotidase (5'N, E.C.3.1.3.5), and lactate (LDH, E.C. 1.1.1.27) and malate (MDH, E.C. 1.1.1.37) dehydrogenase in press-juices of rat brain [8].
• Inhibitors of guanine deaminase are important because it is reasonable to postulate that it will inhibit the conversion of 8-azaguanine to 8-azaxanthine and these could be used with advantage along with thioguanine and 8-azaguanine [6].
• Adenosine deaminase, guanosine deaminase and guanine deaminase were unaffected [9].
• Mobilization of the ribose moiety and of the amino group of guanosine may be realized in rat liver extract by the concerted action of purine nucleoside phosphorylase and guanase [10].

Analytical, diagnostic and therapeutic context of Gda

• Immunohistochemistry using antibodies raised against peptide fragments derived from the guanine deaminase protein sequence showed localization of guanine deaminase in areas predicted by the mRNA distribution [3].

References