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Gene Review

PEP7  -  Pep7p

Saccharomyces cerevisiae S288c

Synonyms: Carboxypeptidase Y-deficient protein 7, Protein VAC1, VAC1, VPL21, VPS19, ...
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High impact information on PEP7

  • Our data establish a molecular link between Vps34 PI(3)K and several FYVE domain-containing proteins (Vac1p, Vps27p) required for vacuolar/lysosomal protein trafficking [1].
  • In semi-intact cells, formation of tubulovesicular structures requires ATP and the proteins encoded by VAC1 and VAC2, two genes which are required for vacuole inheritance in vivo [2].
  • High copy PEP7/VAC1 suppresses vacuolar morphology defects of vps33 mutants [3].
  • We propose that activated-Vps21p interacts with its effector, Vac1p, which interacts with Vps45p to regulate the Golgi to endosome SNARE complex [4].
  • We have constructed an activated allele of VPS21, a yeast Rab protein involved in vacuolar protein sorting, and demonstrated an allele-specific interaction between Vps21p and Vac1p [4].

Biological context of PEP7

  • Temperature-sensitive-for-function (tsf) and dominant negative mutations in PEP12, encoding a putative SNARE vesicle receptor on the endosome, and tsf mutations in VAC1, a gene implicated in vacuole inheritance and vacuolar protein sorting, were constructed and used to demonstrate that Pep12p and Vac1p are components of the VPS pathway [5].
  • The sequence of Vac1p contains two putative zinc-binding RING motifs, a zinc finger motif, and a coiled-coil motif [5].
  • Vac1p FYVE finger mutant missorting phenotypes were suppressed by a defective allele of VPS34 [4].
  • In addition, the loss of Vac1p affected several virulence factors of C. albicans [6].

Anatomical context of PEP7


Associations of PEP7 with chemical compounds


Physical interactions of PEP7


Other interactions of PEP7

  • The functions of Pep12p, Vps45p, and Vps21p indicate that key aspects of Golgi-to-endosome trafficking are similar to other vesicle-mediated transport steps, although the role of Vac1p suggests that there are also novel components of the VPS pathway [5].
  • These fingers also show limited conservation with S. cerevisiae VAC1, Vps11, and Vps18p proteins implicated in vacuolar transport [10].
  • We suggest that the ability of the VPS3, VPS8 and PEP7 genes to rescue lethal effects of oxidative damage resulted from the overexpression of these genes [11].
  • Kinetic studies demonstrate that Pep7p function is required for the transport of the Golgi-precursors of the soluble hydrolases carboxypeptidase Y, proteinase A, and proteinase B to the endosome [8].


  1. Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Burd, C.G., Emr, S.D. Mol. Cell (1998) [Pubmed]
  2. In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae. Conradt, B., Shaw, J., Vida, T., Emr, S., Wickner, W. J. Cell Biol. (1992) [Pubmed]
  3. The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae. Subramanian, S., Woolford, C.A., Jones, E.W. Mol. Biol. Cell (2004) [Pubmed]
  4. The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting. Tall, G.G., Hama, H., DeWald, D.B., Horazdovsky, B.F. Mol. Biol. Cell (1999) [Pubmed]
  5. A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Burd, C.G., Peterson, M., Cowles, C.R., Emr, S.D. Mol. Biol. Cell (1997) [Pubmed]
  6. The vesicle transport protein Vac1p is required for virulence of Candida albicans. Franke, K., Nguyen, M., H??rtl, A., Dahse, H.M., Vogl, G., W??rzner, R., Zipfel, P.F., K??nkel, W., Eck, R. Microbiology (Reading, Engl.) (2006) [Pubmed]
  7. Genetic interactions between a pep7 mutation and the PEP12 and VPS45 genes: evidence for a novel SNARE component in transport between the Saccharomyces cerevisiae Golgi complex and endosome. Webb, G.C., Hoedt, M., Poole, L.J., Jones, E.W. Genetics (1997) [Pubmed]
  8. Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome. Webb, G.C., Zhang, J., Garlow, S.J., Wesp, A., Riezman, H., Jones, E.W. Mol. Biol. Cell (1997) [Pubmed]
  9. The class C Vps complex functions at multiple stages of the vacuolar transport pathway. Peterson, M.R., Emr, S.D. Traffic (2001) [Pubmed]
  10. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. Mu, F.T., Callaghan, J.M., Steele-Mortimer, O., Stenmark, H., Parton, R.G., Campbell, P.L., McCluskey, J., Yeo, J.P., Tock, E.P., Toh, B.H. J. Biol. Chem. (1995) [Pubmed]
  11. Overexpression of genes involved in vesicular trafficking to the vacuole defends against lethal effects of oxidative damage. Martinez, M., Pramanik, A., Moto-Ndje, S., Moore, C.W. Cell. Mol. Biol. (Noisy-le-grand) (2003) [Pubmed]
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