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Gene Review

BUB1  -  Bub1p

Saccharomyces cerevisiae S288c

Synonyms: Checkpoint serine/threonine-protein kinase BUB1, G7542, YGR188C
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High impact information on BUB1

  • Establishment of this Sgo1-binding domain requires the 120-base-pair (bp) core centromere, the kinetochore component Bub1, and the meiosis-specific factor Spo13 [1].
  • Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint [2].
  • The Skp1's interaction with Bub1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization, kinetochore assembly defects, or Mps1 overexpression [2].
  • We propose that the Skp1-Bub1 interaction is important for transmitting a signal to the spindle checkpoint pathway when insufficient tension is present at kinetochores [2].
  • Components of the spindle checkpoint include the mitotic arrest defective (MAD) genes MAD1-3, and the budding uninhibited by benzimidazole (BUB) genes BUB1 and BUB3 [3].

Biological context of BUB1


Anatomical context of BUB1


Associations of BUB1 with chemical compounds


Physical interactions of BUB1

  • Analysis of this activity indicates that the Bub3p-binding domain of Bub1p contributes to this phenotype through disruption of checkpoint activity as well as through introduction of kinetochore or spindle damage [5].

Other interactions of BUB1

  • This suggests that the Bub1p and Mps1p kinases act together at an early step in generating the spindle damage signal [4].
  • The bub1-A78V mutant is of particular interest because it produces a wild-type amount of protein that is mutated in the conserved but uncharacterized Mad3-like region of Bub1p [10].
  • In budding yeast, the Mad1, 2, 3, and Bub1, 2, 3 proteins mediate this arrest [11].
  • We find that three genes (MAD2, BUB1, and BUB2) that are required for the spindle assembly checkpoint in budding yeast (defined by antimicrotubule drug-induced arrest or delay) are also required in the establishment and/or maintenance of kinetochore-induced delays [12].

Analytical, diagnostic and therapeutic context of BUB1


  1. The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I. Kiburz, B.M., Reynolds, D.B., Megee, P.C., Marston, A.L., Lee, B.H., Lee, T.I., Levine, S.S., Young, R.A., Amon, A. Genes Dev. (2005) [Pubmed]
  2. Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Kitagawa, K., Abdulle, R., Bansal, P.K., Cagney, G., Fields, S., Hieter, P. Mol. Cell (2003) [Pubmed]
  3. Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast. Gillett, E.S., Espelin, C.W., Sorger, P.K. J. Cell Biol. (2004) [Pubmed]
  4. Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint. Farr, K.A., Hoyt, M.A. Mol. Cell. Biol. (1998) [Pubmed]
  5. Distinct chromosome segregation roles for spindle checkpoint proteins. Warren, C.D., Brady, D.M., Johnston, R.C., Hanna, J.S., Hardwick, K.G., Spencer, F.A. Mol. Biol. Cell (2002) [Pubmed]
  6. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Roberts, B.T., Farr, K.A., Hoyt, M.A. Mol. Cell. Biol. (1994) [Pubmed]
  7. Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. Bernard, P., Hardwick, K., Javerzat, J.P. J. Cell Biol. (1998) [Pubmed]
  8. Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast. Tournier, S., Gachet, Y., Buck, V., Hyams, J.S., Millar, J.B. Mol. Biol. Cell (2004) [Pubmed]
  9. Bub1 is activated by the protein kinase p90(Rsk) during Xenopus oocyte maturation. Schwab, M.S., Roberts, B.T., Gross, S.D., Tunquist, B.J., Taieb, F.E., Lewellyn, A.L., Maller, J.L. Curr. Biol. (2001) [Pubmed]
  10. The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function. Kadura, S., He, X., Vanoosthuyse, V., Hardwick, K.G., Sazer, S. Mol. Biol. Cell (2005) [Pubmed]
  11. Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. Fraschini, R., Formenti, E., Lucchini, G., Piatti, S. J. Cell Biol. (1999) [Pubmed]
  12. Abnormal kinetochore structure activates the spindle assembly checkpoint in budding yeast. Pangilinan, F., Spencer, F. Mol. Biol. Cell (1996) [Pubmed]
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