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Gene Review

ASF1  -  nucleosome assembly factor ASF1

Saccharomyces cerevisiae S288c

Synonyms: Anti-silencing function protein 1, CIA1, Histone chaperone ASF1, J0755, YJL115W, ...
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High impact information on ASF1


Biological context of ASF1

  • Thus, ASF1-dependent chromatin assembly may mediate the role of the SAS complex in silencing [3].
  • Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription [4].
  • These phenotypes suggested a role for ASF1 in DNA repair and chromosome maintenance [5].
  • The ASF1 protein was evolutionarily conserved, MCB motifs, sequences commonly present upstream of genes transcribed specifically in S phase, were found in front of both genes, and, indeed, both genes were transcribed specifically in the S phase of the cell cycle [5].
  • Two new yeast genes, ASF1 (Anti-Silencing Function) and ASF2, as well as a C-terminal fragment of SIR3, were identified as genes that derepressed the silent mating type loci when overexpressed [5].

Associations of ASF1 with chemical compounds


Physical interactions of ASF1


Regulatory relationships of ASF1

  • In vivo, Asf1p and Hir proteins physically interact and together promote heterochromatic gene silencing in a manner requiring PCNA [7].
  • This Asf1p/H3/H4 complex generated micrococcal nuclease--resistant DNA in the absence of DNA replication and stimulated nucleosome assembly activity by recombinant yeast CAF-I during DNA synthesis [7].
  • These substitution mutations spared the basal interaction with Asf1 and the DNA damage-induced interactions with Rad9 [10].
  • Epistasis analysis indicates that the histone H3 and HAT1 mutants may influence DNA double-strand break repair through Asf1p-dependent chromatin assembly [11].
  • Here we report that overproduction of the chromatin assembly factor Asf1 can suppress the Ts phenotype of mrc1rad53 double mutants and the HU sensitivity of rad53 mutants [12].

Other interactions of ASF1

  • In contrast to the effects of disrupting ASF1 and CHD1, deletion of SNF2 is associated with increased resistance of chromatin to digestion by micrococcal nuclease [13].
  • In vivo, the HIR1 and ASF1 genes contributed to silencing the heterochromatic HML locus via the same genetic pathway [7].
  • ASF2 overexpression caused a greater derepression than did ASF1 [5].
  • Furthermore, increased expression of genes encoding Asf1p, Hir1p, and Hir2p suppressed the apc5(CA) Ts- defect in a CAF-I-dependent manner [14].
  • Conversely, other pol30 alleles prevented Asf1/Hir proteins from contributing to silencing [7].


  1. Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C. Franco, A.A., Lam, W.M., Burgers, P.M., Kaufman, P.D. Genes Dev. (2005) [Pubmed]
  2. The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae. Meijsing, S.H., Ehrenhofer-Murray, A.E. Genes Dev. (2001) [Pubmed]
  3. The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. Osada, S., Sutton, A., Muster, N., Brown, C.E., Yates, J.R., Sternglanz, R., Workman, J.L. Genes Dev. (2001) [Pubmed]
  4. Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription. Sutton, A., Bucaria, J., Osley, M.A., Sternglanz, R. Genetics (2001) [Pubmed]
  5. Two new S-phase-specific genes from Saccharomyces cerevisiae. Le, S., Davis, C., Konopka, J.B., Sternglanz, R. Yeast (1997) [Pubmed]
  6. The Histone Chaperone Anti-silencing Function 1 Stimulates the Acetylation of Newly Synthesized Histone H3 in S-phase. Adkins, M.W., Carson, J.J., English, C.M., Ramey, C.J., Tyler, J.K. J. Biol. Chem. (2007) [Pubmed]
  7. Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Sharp, J.A., Fouts, E.T., Krawitz, D.C., Kaufman, P.D. Curr. Biol. (2001) [Pubmed]
  8. Chromatin assembly factor Asf1p-dependent occupancy of the SAS histone acetyltransferase complex at the silent mating-type locus HMLalpha. Osada, S., Kurita, M., Nishikawa, J., Nishihara, T. Nucleic Acids Res. (2005) [Pubmed]
  9. Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. Krawitz, D.C., Kama, T., Kaufman, P.D. Mol. Cell. Biol. (2002) [Pubmed]
  10. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Lee, S.J., Schwartz, M.F., Duong, J.K., Stern, D.F. Mol. Cell. Biol. (2003) [Pubmed]
  11. Histone H3 and the histone acetyltransferase Hat1p contribute to DNA double-strand break repair. Qin, S., Parthun, M.R. Mol. Cell. Biol. (2002) [Pubmed]
  12. Asf1 links Rad53 to control of chromatin assembly. Hu, F., Alcasabas, A.A., Elledge, S.J. Genes Dev. (2001) [Pubmed]
  13. Replication-independent assembly of nucleosome arrays in a novel yeast chromatin reconstitution system involves antisilencing factor Asf1p and chromodomain protein Chd1p. Robinson, K.M., Schultz, M.C. Mol. Cell. Biol. (2003) [Pubmed]
  14. Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae. Harkness, T.A., Arnason, T.G., Legrand, C., Pisclevich, M.G., Davies, G.F., Turner, E.L. Eukaryotic Cell (2005) [Pubmed]
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