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Gene Review

NAP1  -  histone chaperone NAP1

Saccharomyces cerevisiae S288c

Synonyms: Nucleosome assembly protein, YKR048C
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Disease relevance of NAP1


High impact information on NAP1


Biological context of NAP1

  • Our experiments also suggest that NAP1 is required for the ability of the Clb2/p34CDC28 kinase complex to amplify its own production, and that NAP1 plays a role in regulation of microtubule dynamics during mitosis [7].
  • Together, these results demonstrate that NAP1 is required for the normal function of the activated Clb2/p34CDC28 kinase complex, and provide a step towards understanding how cyclin-dependent kinase complexes induce specific events during the cell cycle [7].
  • This result suggests that yeast NAP1 is required for the maintenance of cumulative nucleosome formation in vivo and the loss of Nap1 leads to a change in the gene expression level in a cluster [8].
  • Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis [9].
  • These results are consistent with a role for plant NAP1-like proteins in cell division [10].

Anatomical context of NAP1


Physical interactions of NAP1


Regulatory relationships of NAP1

  • Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p [9].
  • The results are consistent with the idea that the effects of the spt mutations on nucleosome assembly and/or stability activate repressors of HTA1 transcription [18].
  • Furthermore, we demonstrate that Nap1p promotes the association of the H2A and H2B NLSs specifically with the karyopherin Kap114p [15].
  • Transcription from constructs designed to create steric competition between binding of HSF and histone H2A-H2B dimers was generally poor, suggesting that nucleosome assembly precedes and inhibits HSF binding [19].

Other interactions of NAP1

  • The stable maintenance of Nis1 was dependent on Nap1 [20].
  • In Saccharomyces cerevisiae, additional functions have been ascribed to Nap1, as it has been shown to interact with Clb2 (B type cyclin) and Gin4 (septum formation) [8].
  • We have examined the ability of the yeast mitotic cyclin Clb2 to function in cells that lack NAP1 [7].
  • NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast [7].
  • Consistent with this, we have found that Nap1 is required for viability in cells lacking the redundant G1 cyclins Cln1 and Cln2 [13].

Analytical, diagnostic and therapeutic context of NAP1


  1. Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean. Yoon, H.W., Kim, M.C., Lee, S.Y., Hwang, I., Bahk, J.D., Hong, J.C., Ishimi, Y., Cho, M.J. Mol. Gen. Genet. (1995) [Pubmed]
  2. The N-terminal domains of histones H3 and H4 are not necessary for chromatin assembly factor-1- mediated nucleosome assembly onto replicated DNA in vitro. Shibahara, K., Verreault, A., Stillman, B. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  3. Nucleosome assembly: the CAF and the HAT. Kaufman, P.D. Curr. Opin. Cell Biol. (1996) [Pubmed]
  4. The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae. Meijsing, S.H., Ehrenhofer-Murray, A.E. Genes Dev. (2001) [Pubmed]
  5. Yeast histone H3 and H4 amino termini are important for nucleosome assembly in vivo and in vitro: redundant and position-independent functions in assembly but not in gene regulation. Ling, X., Harkness, T.A., Schultz, M.C., Fisher-Adams, G., Grunstein, M. Genes Dev. (1996) [Pubmed]
  6. Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins. Murzina, N., Verreault, A., Laue, E., Stillman, B. Mol. Cell (1999) [Pubmed]
  7. NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast. Kellogg, D.R., Murray, A.W. J. Cell Biol. (1995) [Pubmed]
  8. Genome-wide expression analysis of NAP1 in Saccharomyces cerevisiae. Ohkuni, K., Shirahige, K., Kikuchi, A. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  9. Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis. Shields, C.M., Taylor, R., Nazarenus, T., Cheatle, J., Hou, A., Tapprich, A., Haifley, A., Atkin, A.L. Curr. Genet. (2003) [Pubmed]
  10. Regulation of biosynthesis and intracellular localization of rice and tobacco homologues of nucleosome assembly protein 1. Dong, A., Zhu, Y., Yu, Y., Cao, K., Sun, C., Shen, W.H. Planta (2003) [Pubmed]
  11. Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay. Meisterernst, M., Horikoshi, M., Roeder, R.G. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  12. Molecular characterization of hNRP, a cDNA encoding a human nucleosome-assembly-protein-I-related gene product involved in the induction of cell proliferation. Simon, H.U., Mills, G.B., Kozlowski, M., Hogg, D., Branch, D., Ishimi, Y., Siminovitch, K.A. Biochem. J. (1994) [Pubmed]
  13. The Sda1 protein is required for passage through start. Zimmerman, Z.A., Kellogg, D.R. Mol. Biol. Cell (2001) [Pubmed]
  14. Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. Krawitz, D.C., Kama, T., Kaufman, P.D. Mol. Cell. Biol. (2002) [Pubmed]
  15. A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. Mosammaparast, N., Ewart, C.S., Pemberton, L.F. EMBO J. (2002) [Pubmed]
  16. Control of mitotic events by Nap1 and the Gin4 kinase. Altman, R., Kellogg, D. J. Cell Biol. (1997) [Pubmed]
  17. Heat shock factor can activate transcription while bound to nucleosomal DNA in Saccharomyces cerevisiae. Pederson, D.S., Fidrych, T. Mol. Cell. Biol. (1994) [Pubmed]
  18. Mutations in the SPT4, SPT5, and SPT6 genes alter transcription of a subset of histone genes in Saccharomyces cerevisiae. Compagnone-Post, P.A., Osley, M.A. Genetics (1996) [Pubmed]
  19. Evidence that partial unwrapping of DNA from nucleosomes facilitates the binding of heat shock factor following DNA replication in yeast. Geraghty, D.S., Sucic, H.B., Chen, J., Pederson, D.S. J. Biol. Chem. (1998) [Pubmed]
  20. Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae. Iwase, M., Toh-e, A. Genes Genet. Syst. (2001) [Pubmed]
  21. Association states of nucleosome assembly protein 1 and its complexes with histones. Tóth, K.F., Mazurkiewicz, J., Rippe, K. J. Biol. Chem. (2005) [Pubmed]
  22. Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. Ishimi, Y., Kikuchi, A. J. Biol. Chem. (1991) [Pubmed]
  23. Purification, crystallization and preliminary X-ray diffraction analysis of yeast nucleosome-assembly factor Cia1p. Padmanabhan, B., Kataoka, K., Adachi, N., Horikoshi, M. Acta Crystallogr. D Biol. Crystallogr. (2002) [Pubmed]
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