The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ASH1  -  Ash1p

Saccharomyces cerevisiae S288c

Synonyms: Daughter cells HO repressor protein, Transcriptional regulatory protein ASH1, YKL185W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ASH1

  • Constitutively expressing an ASH1 mRNA containing the bacteriophage MS2 coat-protein binding site adjacent to the ASH1 3' UTR allowed us to visualize ASH1 mRNA with an MS2-coat-protein-GFP fusion protein (together denoted 'gRNAASH1') [1].
  • Finally, we show that ASH1 is required for full virulence of C. albicans in a mouse model of disseminated candidiasis [2].

High impact information on ASH1


Biological context of ASH1


Anatomical context of ASH1


Physical interactions of ASH1

  • Here we show that She2p is a novel RNA-binding protein that binds specifically to ASH1 mRNA in vivo and to ASH1 RNA zip codes in vitro [11].

Regulatory relationships of ASH1

  • Thus, Ash1p is a cell fate determinant that is asymmetrically localized to the daughter nucleus where it inhibits HO transcription [12].
  • Our epistasis analyses are consistent with the deduction that Ash1 acts separately from the mitogen-activated protein kinase cascade and Ste12 [13].

Other interactions of ASH1

  • A mutant She2 protein that is unable to recognize its cognate target mRNA, ASH1, fails to localize Myo4p [14].
  • She2p can interact with She3 protein via She3p's C-terminus and becomes localized to the daughter cell tip upon ASH1 expression [11].
  • In pho85 mutants, Ash1 protein is no longer asymmetrically localized and is present, instead, in both mother and daughter cells [8].
  • We describe a gene, ASH1, that is necessary to repress HO in daughters [5].
  • In budding yeast, ASH1 mRNA is translocated via actin and myosin to the tip of growing cells [15].

Analytical, diagnostic and therapeutic context of ASH1

  • Co-immunoprecipitation studies also indicated that Khd1 associates with ASH1 mRNA through the N element [16].
  • A GFP-decorated reporter transcript containing the ASH1 3' untranslated region gRNA(ASH1) forms spots of fluorescence localized to a cortical domain at the bud tip, relocates to the mother-bud neck before cell separation, and finally migrates to the incipient bud site before the next budding cycle [15].


  1. Localization and anchoring of mRNA in budding yeast. Beach, D.L., Salmon, E.D., Bloom, K. Curr. Biol. (1999) [Pubmed]
  2. Ash1 protein, an asymmetrically localized transcriptional regulator, controls filamentous growth and virulence of Candida albicans. Inglis, D.O., Johnson, A.D. Mol. Cell. Biol. (2002) [Pubmed]
  3. She2p is a novel RNA binding protein with a basic helical hairpin motif. Niessing, D., Hüttelmaier, S., Zenklusen, D., Singer, R.H., Burley, S.K. Cell (2004) [Pubmed]
  4. Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cosma, M.P., Tanaka, T., Nasmyth, K. Cell (1999) [Pubmed]
  5. Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells. Bobola, N., Jansen, R.P., Shin, T.H., Nasmyth, K. Cell (1996) [Pubmed]
  6. A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Gu, W., Deng, Y., Zenklusen, D., Singer, R.H. Genes Dev. (2004) [Pubmed]
  7. An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. Long, R.M., Gu, W., Meng, X., Gonsalvez, G., Singer, R.H., Chartrand, P. J. Cell Biol. (2001) [Pubmed]
  8. The protein kinase Pho85 is required for asymmetric accumulation of the Ash1 protein in Saccharomyces cerevisiae. McBride, H.J., Sil, A., Measday, V., Yu, Y., Moffat, J., Maxon, M.E., Herskowitz, I., Andrews, B., Stillman, D.J. Mol. Microbiol. (2001) [Pubmed]
  9. Localization of ASH1 mRNA particles in living yeast. Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S.M., Singer, R.H., Long, R.M. Mol. Cell (1998) [Pubmed]
  10. Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast. Takizawa, P.A., Sil, A., Swedlow, J.R., Herskowitz, I., Vale, R.D. Nature (1997) [Pubmed]
  11. She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p. Böhl, F., Kruse, C., Frank, A., Ferring, D., Jansen, R.P. EMBO J. (2000) [Pubmed]
  12. Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene. Sil, A., Herskowitz, I. Cell (1996) [Pubmed]
  13. Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae. Chandarlapaty, S., Errede, B. Mol. Cell. Biol. (1998) [Pubmed]
  14. Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p. Kruse, C., Jaedicke, A., Beaudouin, J., Bohl, F., Ferring, D., Guttler, T., Ellenberg, J., Jansen, R.P. J. Cell Biol. (2002) [Pubmed]
  15. ASH1 mRNA localization in three acts. Beach, D.L., Bloom, K. Mol. Biol. Cell (2001) [Pubmed]
  16. The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast. Irie, K., Tadauchi, T., Takizawa, P.A., Vale, R.D., Matsumoto, K., Herskowitz, I. EMBO J. (2002) [Pubmed]
WikiGenes - Universities