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Gene Review

TRP5  -  tryptophan synthase TRP5

Saccharomyces cerevisiae S288c

Synonyms: Tryptophan synthase, YGL026C
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Disease relevance of TRP5


High impact information on TRP5

  • Expression of the yeast tryptophan synthetase activity from the cloned yeast gene (trp5) is relatively inefficient in E. coli, as measured by growth rates of trpAB/pYe(trp5)1 strains on minimal media lacking tryptophan and by enzyme assays [3].
  • Plasmid DNA [pYe(trp5)2] from one of these variants was shown to contain a DNA insertion (1.3 kilobase pairs) in the cloned yeast DNA segment in relatively close proximity to the trp5 gene [3].
  • Control of expression of a cloned yeast (Saccharomyces cerevisiae) gene (trp5) by a bacterial insertion element (IS2) [3].
  • The peak reversion frequency for lys5 is somewhat later, while the peaks for tyr3 and trp5 occur near the end of DNA replication [4].
  • The R388 plasmid-encoded drug-resistant type II dihydrofolate reductase gene (R . dhfr) was expressed in Saccharomyces cerevisiae by fusing the R . dhfr coding sequence to the yeast TRP5 promoter [5].

Chemical compound and disease context of TRP5


Biological context of TRP5


Associations of TRP5 with chemical compounds


Physical interactions of TRP5

  • During mitosis, gene conversion events at the TRP5 locus on chromosome VII are coupled with conversion events at LEU1, a locus 18 cM away, 1200 times more frequently than would be expected for two independent acts of recombination [16].

Regulatory relationships of TRP5


Other interactions of TRP5

  • Within a 31 kb region from PMA1 towards TRP5, a total of 12 transcription products ranging from 0.6 to 3.6 kb were identified in cells grown exponentially on rich medium [9].
  • The plasmid-borne genes TRP2 to TRP5 were expressed and regulated normally in the frame of the general control [6].
  • In yeast, coincident gene conversion events involving the LEU1 and TRP5 loci (16 cM apart) occur at frequencies that are far greater than is expected for two independent acts of recombination [18].
  • The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain [19].
  • The systems comprise auxotrophic host strains (trp5 in the case of S. occidentalis; trp5-10, his3 in the case of P. stipitis) and suitable transformation vectors [12].


  1. DNA sequences flanking an E. coli insertion element IS2 in a cloned yeast TRP5 gene. Brosius, J., Walz, A. Gene (1982) [Pubmed]
  2. Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa. Hadero, A., Crawford, I.P. Mol. Biol. Evol. (1986) [Pubmed]
  3. Control of expression of a cloned yeast (Saccharomyces cerevisiae) gene (trp5) by a bacterial insertion element (IS2). Walz, A., Ratzkin, B., Carbon, J. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  4. Cell cycle-dependent induction of mutations along a yeast chromosome. Kee, S.G., Haber, J.E. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  5. Expression of plasmid R388-encoded type II dihydrofolate reductase as a dominant selective marker in Saccharomyces cerevisiae. Miyajima, A., Miyajima, I., Arai, K., Arai, N. Mol. Cell. Biol. (1984) [Pubmed]
  6. Expression of an artificial yeast TRP-gene cluster in yeast and Escherichia coli. Niederberger, P., Aebi, M., Furter, R., Prantl, F., Hütter, R. Mol. Gen. Genet. (1984) [Pubmed]
  7. Yeast gene TRP5: structure, function, regulation. Zalkin, H., Yanofsky, C. J. Biol. Chem. (1982) [Pubmed]
  8. L-serine binds to arginine-148 of the beta 2 subunit of Escherichia coli tryptophan synthase. Tanizawa, K., Miles, E.W. Biochemistry (1983) [Pubmed]
  9. Physical, transcriptional and genetical mapping of a 24 kb DNA fragment located between the PMA1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae. Capieaux, E., Ulaszewski, S., Balzi, E., Goffeau, A. Yeast (1991) [Pubmed]
  10. The predicted presence of large helical structural variation in yeast HIS4 upstream region is correlated with general amino acid control on the CYC1 gene. Nussinov, R. J. Biomol. Struct. Dyn. (1985) [Pubmed]
  11. Nucleotide sequence of the Neurospora crassa trp-3 gene encoding tryptophan synthetase and comparison of the trp-3 polypeptide with its homologs in Saccharomyces cerevisiae and Escherichia coli. Burns, D.M., Yanofsky, C. J. Biol. Chem. (1989) [Pubmed]
  12. Two novel gene expression systems based on the yeasts Schwanniomyces occidentalis and Pichia stipitis. Piontek, M., Hagedorn, J., Hollenberg, C.P., Gellissen, G., Strasser, A.W. Appl. Microbiol. Biotechnol. (1998) [Pubmed]
  13. The detection of monosomic colonies produced by mitotic chromosome non-disjunction in the yeast Saccharomyces cerevisiae. Parry, J.M., Zimmerman, F.K. Mutat. Res. (1976) [Pubmed]
  14. Mitotic gene conversion induced in yeast by isoniazid. influence of a transition metal and of the physiological conditions of the cells. Zetterberg, G., Boström, G. Mutat. Res. (1981) [Pubmed]
  15. Mutagenesis of Saccharomyces cerevisiae by sodium azide activated in barley. Velemínský, J., Silhánková, L., Smiovská, V., Gichner, T. Mutat. Res. (1979) [Pubmed]
  16. Coincident gene conversion during mitosis in saccharomyces. Golin, J.E., Esposito, M.S. Genetics (1984) [Pubmed]
  17. Formation of a complex between yeast proteinases A and B. Hinze, H., Betz, H., Saheki, T., Holzer, H. Hoppe-Seyler's Z. Physiol. Chem. (1975) [Pubmed]
  18. The behavior of insertions near a site of mitotic gene conversion in yeast. Golin, J.E., Falco, S.C. Genetics (1988) [Pubmed]
  19. The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Balzi, E., Chen, W.N., Capieaux, E., McCusker, J.H., Haber, J.E., Goffeau, A. Gene (1989) [Pubmed]
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