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Gene Review

RNASET2  -  ribonuclease T2

Homo sapiens

Synonyms: FLJ10907, RNASE6PL, Ribonuclease 6, Ribonuclease T2, bA514O12.3
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Disease relevance of RNASET2

  • Tumor and metastasis suppression by the human RNASET2 gene [1].
  • Moreover, we have extended our previous studies by evaluating the effect of RNASET2 on the metastatic behavior of the highly-invasive ovarian cancer cell line HEY3MET2 [1].
  • RNASET2 is endowed with catalytic activity as demonstrated with purified recombinant protein expressed in the Baculovirus Expression Vector System and in a human cell line ectopically expressing various types of constructs [2].

High impact information on RNASET2

  • Enzyme catalyzed hydrolysis of the nonamers with ribonuclease T2 showed that the linkage at the ribonucleotide was the desired 3'-5'. A control nonamer with a 2'-5' linkage was subjected to the enzyme, and showed no cleavage [3].
  • Digestion with ribonuclease T2 has been used to study the size of poly(U) protected by ribosome binding [4].
  • We show that RNASET2 is present in multiple forms in human cell lines and mouse tissues, one of which represents the full length, glycosylated and secreted form, while the others are proteolytic products [2].
  • In this study, we have started the cellular and biochemical characterization of RNASET2 and showed that it is a secreted glycoprotein [1].
  • Moreover, RNASET2-mediated suppression of tumorigenesis and metastasis was not affected by a double point mutation targeted to the putative ribonuclease catalytic sites, suggesting that tumor suppression by RNASET2 is not mediated by its ribonuclease activity [1].

Biological context of RNASET2


Anatomical context of RNASET2


Associations of RNASET2 with chemical compounds

  • BACKGROUND.: A synthetic RNASET2 gene that was optimized for expression in the yeast Pichia pastoris was designed according to the cDNA sequence and was cloned under the control of the methanol-induced promoter fused to the alpha-mating secretion peptide [5].
  • Production of the substrate to a high degree of purity has been achieved using apo-glucose oxidase to specifically remove contaminating flavin adenine dinucleotide from solution and hydrolysis of a cyclic intermediate as a final production protocol by ribonuclease T2 to give the product in high yield [8].

Other interactions of RNASET2

  • In HUVE cells, RNASET2 inhibited angiogenin-, bFGF-, and VEGF-induced tube formation in a dose-dependent manner [5].
  • Because the homozygous deletion might affect the expression of nearby genes, we analyzed the expression of the two closest known genes flanking the deletion, RNASE6PL and RSK-3 [9].

Analytical, diagnostic and therapeutic context of RNASET2


  1. Tumor and metastasis suppression by the human RNASET2 gene. Acquati, F., Possati, L., Ferrante, L., Campomenosi, P., Talevi, S., Bardelli, S., Margiotta, C., Russo, A., Bortoletto, E., Rocchetti, R., Calza, R., Cinquetti, R., Monti, L., Salis, S., Barbanti-Brodano, G., Taramelli, R. Int. J. Oncol. (2005) [Pubmed]
  2. Characterization of RNASET2, the first human member of the Rh/T2/S family of glycoproteins. Campomenosi, P., Salis, S., Lindqvist, C., Mariani, D., Nordström, T., Acquati, F., Taramelli, R. Arch. Biochem. Biophys. (2006) [Pubmed]
  3. Enzymatic and NMR analysis of oligoribonucleotides synthesized with 2'-tert-butyldimethylsilyl protected cyanoethylphosphoramidite monomers. Wang, Y.Y., Lyttle, M.H., Borer, P.N. Nucleic Acids Res. (1990) [Pubmed]
  4. Structure of ribosome-bound messenger RNA as revealed by enzymatic accessibility studies. Kang, C.W., Cantor, C.R. J. Mol. Biol. (1985) [Pubmed]
  5. A recombinant human RNASET2 glycoprotein with antitumorigenic and antiangiogenic characteristics: expression, purification, and characterization. Smirnoff, P., Roiz, L., Angelkovitch, B., Schwartz, B., Shoseyov, O. Cancer (2006) [Pubmed]
  6. Steady-state kinetic studies of binding and catalysis by ribonuclease T2: a microenvironmental survey of the active site by using a series of adenosine-3'- and -5'-alkylphosphates. Yasuda, T., Inoue, Y. J. Biochem. (1983) [Pubmed]
  7. Comparative study of proteome between primary cancer and hepatic metastatic tumor in colorectal cancer. Yu, B., Li, S.Y., An, P., Zhang, Y.N., Liang, Z.J., Yuan, S.J., Cai, H.Y. World J. Gastroenterol. (2004) [Pubmed]
  8. Purification and semienzymic synthesis of flavin adenine dinucleotide-3'-phosphate. Fisher, M., Harbron, S., Eggelte, H.J., Rabin, B.R. Enzyme Microb. Technol. (1994) [Pubmed]
  9. A novel homozygous deletion at chromosomal band 6q27 in an ovarian cancer cell line delineates the position of a putative tumor suppressor gene. Lin, H., Morin, P.J. Cancer Lett. (2001) [Pubmed]
  10. Partial purification and immobilization of ribonuclease T2. Leon, D., Gite, S., Shankar, V. Biotechnol. Appl. Biochem. (1992) [Pubmed]
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