The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

HT29 Cells

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of HT29 Cells


High impact information on HT29 Cells

  • The only receptor consistently detected was CXCR4 (fusin/LESTR), although HT-29 cells did not express mRNA for its ligand, stromal cell-derived factor (SDF-1alpha) [6].
  • Butyrate but not acetate or proprionate decreased basal and cAMP-stimulated bumetanide-sensitive K+ (86Rb) uptake in both HT29 cells and the Cl--secreting T84 line [7].
  • RESULTS: Clinically relevant concentrations of cisplatin, doxorubicin, mitomycin C, fluorouracil, or camptothecin enhanced Fas receptor expression on the plasma membrane of HT29 cells [8].
  • We also assessed the ability of UCN-01 to enhance the cytotoxicity of gamma irradiation in CA46 cells and human colon carcinoma HT-29 cells, both of which are mutant for p53 function [9].
  • TNF increased VCAM-1 expression to 4.2 times the vehicle-treated control levels (P < .05) on HT-29 cells and increased ICAM-1 expression on HT-29, LoVo, and SW-620 cells (8.4, 1.8, and 1.9 times vehicle control levels, respectively; P < .05 in each case) [10].

Chemical compound and disease context of HT29 Cells

  • For example, HT29 cells (alkyltransferase activity, 381 fmol/mg protein) exhibited a greater degree of enhancement when treated with O6-benzylguanine than SF767 (77 fmol/mg protein) and M19-MEL melanoma (36 fmol/mg protein) cells [11].
  • To test this hypothesis, we treated HT29 and SW620 human colon cancer cells with fluorodeoxyuridine under conditions that produced nearly complete inhibition of thymidylate synthase but which sensitized only the HT29 cells [12].
  • Cellular invasion induced by 10 ng/ml stem cell factor (EC(50) = 3 ng/ml) in HT29 cells was blocked by 1 micro M STI571 (IC(50) = 56 nM) and pharmacological inhibitors of several oncogenic signaling pathways, namely, phosphatidylinositol 3-kinase (LY294002), Rho GTPases (Clostridium botulinum exoenzyme C3 transferase), and Rho-kinase (Y27632) [13].
  • Taken together, these results suggest that suramin blocks HIV-1 infection in HT-29 cells because it binds to the V3 domain of gp120 and hence prevents the interaction between gp120 and the GalCer receptor [14].
  • 1-Deoxymannojirimycin (dMM), a specific alpha-mannosidase I inhibitor, completely blocks the conversion of Man9-8GlcNAc2 into Man7-5-GlcNAc2 in both differentiated and undifferentiated human adenocarcinoma HT-29 cells [15].

Biological context of HT29 Cells


Anatomical context of HT29 Cells


Associations of HT29 Cells with chemical compounds

  • HT-29 cells induced to differentiate by another signal, glucose deprivation, upregulated receptor content and response to toxin [25].
  • The effect of UCN-01 on the cytotoxicity of gamma irradiation in CA46 and HT-29 cells was determined by use of MTT (thiazolyl blue) and clonogenic (colony-forming) assays, respectively; a clonogenic assay was also used to measure the effect of UCN-01 on the cytotoxicity of cisplatin in transfected and nontransfected MCF-7 cells [9].
  • METHODS: A clinical phase I study of STZ followed by BCNU was designed to simulate conditions that produce maximal sensitization of CENU-resistant HT-29 cells in vitro [26].
  • In contrast, neither STa nor guanylin changed the uptake of taurine in HT-29 cells [27].
  • RESULTS: SN-38 and CPT were more cytotoxic than 9-AC and TPT, and CPT-11 was almost inactive toward HT-29 cells [28].

Gene context of HT29 Cells

  • NF-kappaB binding activity was unaltered by PI 3-kinase inhibition in HT-29 cells, in which TNF-alpha was shown to activate PI 3-kinase directly [29].
  • RESULTS: TNF-alpha and IL-1 beta dose-dependently induced IL-8 production in HT-29 cells [30].
  • IELs lysed more 51Cr-labeled HT-29 cells when cultured for 72 hours with IL-15 (48% +/- 3% at 25:1 effector-to-target ratio) than with IL-2 (27% +/- 3%) or IL-7 (12% +/- 2%) (P < 0.0001) [31].
  • When the antisense orientation of the VIP receptor clone was introduced into HT-29 cells, there was a 50% suppression of the specific binding of 125I-VIP and of the VIP-induced increase in cAMP level, relative to untransfected cells [22].
  • Mice bearing EGFR+ tumor cells lived significantly (P less than 0.001) longer when treated with TGF alpha-PE40 delta cys compared with saline-treated controls (median survival: A431 cells, 51.5 vs. 25.5 days; HT29 cells, 101 vs. 47.5 days) [32].

Analytical, diagnostic and therapeutic context of HT29 Cells


  1. Proliferative responses of HT29 and Caco2 human colorectal cancer cells to a panel of lectins. Ryder, S.D., Smith, J.A., Rhodes, E.G., Parker, N., Rhodes, J.M. Gastroenterology (1994) [Pubmed]
  2. Anti-cell surface monoclonal antibodies which antagonize the action of VIP in a human adenocarcinoma cell line (HT 29 cells). Pichon, J., Hirn, M., Muller, J.M., Mangeat, P., Marvaldi, J. EMBO J. (1983) [Pubmed]
  3. Activation of AP-1 and of a nuclear redox factor, Ref-1, in the response of HT29 colon cancer cells to hypoxia. Yao, K.S., Xanthoudakis, S., Curran, T., O'Dwyer, P.J. Mol. Cell. Biol. (1994) [Pubmed]
  4. Glucuronidation as a mechanism of intrinsic drug resistance in human colon cancer: reversal of resistance by food additives. Cummings, J., Ethell, B.T., Jardine, L., Boyd, G., Macpherson, J.S., Burchell, B., Smyth, J.F., Jodrell, D.I. Cancer Res. (2003) [Pubmed]
  5. Differential cytotoxicity and DNA-damaging effects produced in human cells of the Mer+ and Mer- phenotypes by a series of 1-aryl-3-alkyltriazenes. Gibson, N.W., Hartley, J.A., LaFrance, R.J., Vaughan, K. Cancer Res. (1986) [Pubmed]
  6. Expression of functional CXCR4 chemokine receptors on human colonic epithelial cells. Jordan, N.J., Kolios, G., Abbot, S.E., Sinai, M.A., Thompson, D.A., Petraki, K., Westwick, J. J. Clin. Invest. (1999) [Pubmed]
  7. Na-K-2Cl cotransporter gene expression and function during enterocyte differentiation. Modulation of Cl- secretory capacity by butyrate. Matthews, J.B., Hassan, I., Meng, S., Archer, S.Y., Hrnjez, B.J., Hodin, R.A. J. Clin. Invest. (1998) [Pubmed]
  8. Sensitization of cancer cells treated with cytotoxic drugs to fas-mediated cytotoxicity. Micheau, O., Solary, E., Hammann, A., Martin, F., Dimanche-Boitrel, M.T. J. Natl. Cancer Inst. (1997) [Pubmed]
  9. UCN-01: a potent abrogator of G2 checkpoint function in cancer cells with disrupted p53. Wang, Q., Fan, S., Eastman, A., Worland, P.J., Sausville, E.A., O'Connor, P.M. J. Natl. Cancer Inst. (1996) [Pubmed]
  10. Levamisole effects on major histocompatibility complex and adhesion molecule expression and on myeloid cell adhesion to human colon tumor cell lines. Kimball, E.S., Fisher, M.C. J. Natl. Cancer Inst. (1996) [Pubmed]
  11. Effect of O6-benzylguanine analogues on sensitivity of human tumor cells to the cytotoxic effects of alkylating agents. Dolan, M.E., Mitchell, R.B., Mummert, C., Moschel, R.C., Pegg, A.E. Cancer Res. (1991) [Pubmed]
  12. Fluoropyrimidine-mediated radiosensitization depends on cyclin E-dependent kinase activation. Lawrence, T.S., Davis, M.A., Loney, T.L. Cancer Res. (1996) [Pubmed]
  13. The c-kit tyrosine kinase inhibitor STI571 for colorectal cancer therapy. Attoub, S., Rivat, C., Rodrigues, S., Van Bocxlaer, S., Bedin, M., Bruyneel, E., Louvet, C., Kornprobst, M., André, T., Mareel, M., Mester, J., Gespach, C. Cancer Res. (2002) [Pubmed]
  14. Suramin inhibits binding of the V3 region of HIV-1 envelope glycoprotein gp120 to galactosylceramide, the receptor for HIV-1 gp120 on human colon epithelial cells. Yahi, N., Sabatier, J.M., Nickel, P., Mabrouk, K., Gonzalez-Scarano, F., Fantini, J. J. Biol. Chem. (1994) [Pubmed]
  15. Dual effect of 1-deoxymannojirimycin on the mannose uptake and on the N-glycan processing of the human colon cancer cell line HT-29. Ogier-Denis, E., Trugnan, G., Sapin, C., Aubery, M., Codogno, P. J. Biol. Chem. (1990) [Pubmed]
  16. Leptin is a growth factor for colonic epithelial cells. Hardwick, J.C., Van Den Brink, G.R., Offerhaus, G.J., Van Deventer, S.J., Peppelenbosch, M.P. Gastroenterology (2001) [Pubmed]
  17. Down-regulation of cystic fibrosis transmembrane conductance regulator gene expression by agents that modulate intracellular divalent cations. Bargon, J., Trapnell, B.C., Chu, C.S., Rosenthal, E.R., Yoshimura, K., Guggino, W.B., Dalemans, W., Pavirani, A., Lecocq, J.P., Crystal, R.G. Mol. Cell. Biol. (1992) [Pubmed]
  18. Functional annotation of a novel NFKB1 promoter polymorphism that increases risk for ulcerative colitis. Karban, A.S., Okazaki, T., Panhuysen, C.I., Gallegos, T., Potter, J.J., Bailey-Wilson, J.E., Silverberg, M.S., Duerr, R.H., Cho, J.H., Gregersen, P.K., Wu, Y., Achkar, J.P., Dassopoulos, T., Mezey, E., Bayless, T.M., Nouvet, F.J., Brant, S.R. Hum. Mol. Genet. (2004) [Pubmed]
  19. Src activity increases and Yes activity decreases during mitosis of human colon carcinoma cells. Park, J., Cartwright, C.A. Mol. Cell. Biol. (1995) [Pubmed]
  20. Antineoplastic drugs sulindac sulfide and sulfone inhibit cell growth by inducing apoptosis. Piazza, G.A., Rahm, A.L., Krutzsch, M., Sperl, G., Paranka, N.S., Gross, P.H., Brendel, K., Burt, R.W., Alberts, D.S., Pamukcu, R. Cancer Res. (1995) [Pubmed]
  21. Apical expression of functional asialoglycoprotein receptor in the human intestinal cell line HT-29. Mu, J.Z., Gordon, M., Shao, J.S., Alpers, D.H. Gastroenterology (1997) [Pubmed]
  22. Cloning and expression of the human vasoactive intestinal peptide receptor. Sreedharan, S.P., Robichon, A., Peterson, K.E., Goetzl, E.J. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  23. Inhibition of COX-2 in colon cancer cell lines by celecoxib increases the nuclear localization of active p53. Swamy, M.V., Herzog, C.R., Rao, C.V. Cancer Res. (2003) [Pubmed]
  24. Glucuronidation associated with intrinsic resistance to mycophenolic acid in human colorectal carcinoma cells. Franklin, T.J., Jacobs, V., Jones, G., Plé, P., Bruneau, P. Cancer Res. (1996) [Pubmed]
  25. Maturational regulation of globotriaosylceramide, the Shiga-like toxin 1 receptor, in cultured human gut epithelial cells. Jacewicz, M.S., Acheson, D.W., Mobassaleh, M., Donohue-Rolfe, A., Balasubramanian, K.A., Keusch, G.T. J. Clin. Invest. (1995) [Pubmed]
  26. Phase I study of streptozocin- and carmustine-sequenced administration in patients with advanced cancer. Micetich, K.C., Futscher, B., Koch, D., Fisher, R.I., Erickson, L.C. J. Natl. Cancer Inst. (1992) [Pubmed]
  27. Regulation of taurine transport by Escherichia coli heat-stable enterotoxin and guanylin in human intestinal cell lines. Brandsch, M., Ramamoorthy, S., Marczin, N., Catravas, J.D., Leibach, J.W., Ganapathy, V., Leibach, F.H. J. Clin. Invest. (1995) [Pubmed]
  28. Comparison of topoisomerase I inhibition, DNA damage, and cytotoxicity of camptothecin derivatives presently in clinical trials. Tanizawa, A., Fujimori, A., Fujimori, Y., Pommier, Y. J. Natl. Cancer Inst. (1994) [Pubmed]
  29. Regulatory role of phosphatidylinositol 3-kinase on TNF-alpha-induced cyclooxygenase 2 expression in colonic epithelial cells. Weaver, S.A., Russo, M.P., Wright, K.L., Kolios, G., Jobin, C., Robertson, D.A., Ward, S.G. Gastroenterology (2001) [Pubmed]
  30. Regulation of interleukin-8 production in a human colon epithelial cell line (HT-29). Gross, V., Andus, T., Daig, R., Aschenbrenner, E., Schölmerich, J., Falk, W. Gastroenterology (1995) [Pubmed]
  31. Interleukin 15 is a potent stimulant of intraepithelial lymphocytes. Ebert, E.C. Gastroenterology (1998) [Pubmed]
  32. Transforming growth factor alpha-Pseudomonas exotoxin fusion protein prolongs survival of nude mice bearing tumor xenografts. Heimbrook, D.C., Stirdivant, S.M., Ahern, J.D., Balishin, N.L., Patrick, D.R., Edwards, G.M., Defeo-Jones, D., FitzGerald, D.J., Pastan, I., Oliff, A. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  33. A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6. Lehmann, M., Rabenandrasana, C., Tamura, R., Lissitzky, J.C., Quaranta, V., Pichon, J., Marvaldi, J. Cancer Res. (1994) [Pubmed]
  34. Differences in lipid characteristics of undifferentiated and enterocytic-differentiated HT29 human colonic cells. Reynier, M., Sari, H., d'Anglebermes, M., Kye, E.A., Pasero, L. Cancer Res. (1991) [Pubmed]
  35. Divergent induction of apoptosis and IL-8 secretion in HT-29 cells in response to TNF-alpha and ligation of Fas antigen. Abreu-Martin, M.T., Vidrich, A., Lynch, D.H., Targan, S.R. J. Immunol. (1995) [Pubmed]
  36. Induction of cyclin E and inhibition of DNA synthesis by the novel acronycine derivative S23906-1 precede the irreversible arrest of tumor cells in S phase leading to apoptosis. Léonce, S., Pérez, V., Lambel, S., Peyroulan, D., Tillequin, F., Michel, S., Koch, M., Pfeiffer, B., Atassi, G., Hickman, J.A., Pierré, A. Mol. Pharmacol. (2001) [Pubmed]
  37. Aggregative adherence fimbriae contribute to the inflammatory response of epithelial cells infected with enteroaggregative Escherichia coli. Harrington, S.M., Strauman, M.C., Abe, C.M., Nataro, J.P. Cell. Microbiol. (2005) [Pubmed]
WikiGenes - Universities