High impact information on ABCC10

• Analysis of the drug resistance profile of multidrug resistance protein 7 (ABCC10): resistance to docetaxel [1].
• Characterization of the transport properties of human multidrug resistance protein 7 (MRP7, ABCC10) [2].
• Basal MRP7 gene expression was regulated by 2 regions in the 5'-flanking region at -1,780-1,287 bp, and at -611 to -208 bp [3].
• The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter [3].
• The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily [3].

Biological context of ABCC10

• Recent studies have begun to show the broad tissue distribution and drug substrate specificity of the seven MRP family members (MRP1-7; or ABCC1-6 and ABCC10) [4].
• The ABCC10 cDNA was cloned in the human full-length cDNA project at the Kazusa DNA Research Institute. However, current information is limited regarding its physiological function and gene expression [5].
• These results suggest that expression of the ABCC10 gene is regulated in a p53-dependent manner during DNA-damage-related apoptosis [5].
• Furthermore, when we examined its expression under apoptotic conditions, we found that ABCC10 mRNA levels remarkably increased in doxorubicin-treated MCF7 cells, whereas its up-regulation was suppressed in p53-dominant-negative MCF7 cells [5].
• Fluorescence in situ hybridization indicated that MRP7 maps to chromosome 6p12-21, in proximity to several genes associated with glutathione conjugation and synthesis [6].

Anatomical context of ABCC10

• By quantitative PCR, ABCC10 gene expression is demonstrated to be highest in pancreas among the adult and fetal tissues and tumors presently tested [5].
• The integrity of the predicted MRP7 coding sequence was confirmed by the synthesis of an approximately 158 kDa protein in reticulocyte lysates programmed with the MRP7 cDNA [6].

Associations of ABCC10 with chemical compounds

• However, in contrast to the latter transporters, a conserved N-linked glycosylation site is not found at the N-terminus of MRP7 [6].
• In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226% by genotoxic 2-acetylaminofluorene and 347% by the histone deacetylase inhibitor, trichostatin A [3].

Other interactions of ABCC10

• Analysis of the structure and expression pattern of MRP7 (ABCC10), a new member of the MRP subfamily [6].

Analytical, diagnostic and therapeutic context of ABCC10

• While MRP7 transcript was detected in a variety of tissues by RT/PCR, it was not readily detectable by RNA blot analysis, suggesting that it is expressed at low levels in these tissues [6].

References