Disease relevance of CamMLVgp102

• Vaccinia DNA topoisomerase, a eukaryotic type I enzyme, binds and cleaves duplex DNA at sites containing the sequence 5'-(T/C)CCTT [1].
• Vaccinia DNA topoisomerase I promotes illegitimate recombination in Escherichia coli [2].
• This suggests that transcription of ASF virus DNA requires a DNA topoisomerase [3].

High impact information on CamMLVgp102

• A catalytic domain of eukaryotic DNA topoisomerase I [4].
• Vaccinia DNA topoisomerase, a member of the eukaryotic type I enzyme family, binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'-CCCTT decreases in the scissile strand [5].
• The ability of a eukaryotic DNA topoisomerase I to catalyze DNA rearrangements was examined in vitro using defined substrates and purified enzyme [6].
• Replacement of the active site tyrosine of vaccinia DNA topoisomerase by glutamate, cysteine or histidine converts the enzyme into a site-specific endonuclease [7].
• Here, we examine the effects of intercalating PAH-DNA adducts on the religation reaction of vaccinia DNA topoisomerase, a prototypal type IB topoisomerase (TopIB), and the 3' end-resection reaction of Escherichia coli exonuclease III (ExoIII), a DNA repair enzyme [8].

Chemical compound and disease context of CamMLVgp102

• Mechanism of inhibition of vaccinia DNA topoisomerase by novobiocin and coumermycin [9].
• Vaccinia DNA topoisomerase, a eukaryotic type I enzyme, has unique pharmacological properties, including sensitivity to the coumarin drugs novobiocin and coumermycin, which are classical inhibitors of DNA gyrase, a type II enzyme [9].
• We have used potassium permanganate to probe contacts between vaccinia DNA topoisomerase and thymine residues in its 5'-CCCTT downward arrow DNA target site [10].

Biological context of CamMLVgp102

• Vaccinia DNA topoisomerase specifically binds and forms a covalent adduct at DNA sites containing a conserved sequence element 5'(C/T)CCTT decreases in the scissile strand [11].
• These results suggest that the vaccinia DNA topoisomerase may play a role in the ATP-dependent transcription of viral genes from intact core particles [12].
• Vaccinia virus encodes a type I DNA topoisomerase whose function in virus replication is not known [13].
• Insertional mutagenesis of the vaccinia virus gene encoding a type I DNA topoisomerase: evidence that the gene is essential for virus growth [13].

Associations of CamMLVgp102 with chemical compounds

• Fluorometric methods employing low concentrations of ethidium bromide for DNA topoisomerase and endonuclease assays [14].

Analytical, diagnostic and therapeutic context of CamMLVgp102

• Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase [15].
• Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactions [16].
• DNA topoisomerase activity can be rapidly assayed by measuring the change in ethidium bromide fluorescence intensity after treatment of closed duplex DNA with enzyme [14].

References