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Chemical Compound Review

Hydroethidine     5-ethyl-6-phenyl-6H- phenanthridine-3,8...

Synonyms: SureCN79004, D7008_SIAL, CTK8D4610, AR-1G6158, AKOS015915966, ...
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Disease relevance of ETHIDIUM BROMIDE


Psychiatry related information on ETHIDIUM BROMIDE

  • The experimental variables, i.e., pH of the buffer solution, the concentration and volume of EB solution, the reaction time and the fluid flow rates, were investigated [5].

High impact information on ETHIDIUM BROMIDE

  • Ethidium bromide reduces the formation of superhelical viral DNA and concurrently blocks integration of the viral genome [1].
  • Two human cell lines (termed rho 0), which had been completely depleted of mitochondrial DNA (mtDNA) by long-term exposure to ethidium bromide, were found to be dependent on uridine and pyruvate for growth because of the absence of a functional respiratory chain [6].
  • Among different geographic isolates of the same subspecies, more than two-thirds of chromosomes had similar sizes, ethidium bromide staining intensities, and locations of alpha,beta-tubulin genes [7].
  • Viable Leishmania promastigotes and amastigotes were detected by epifluorescence microscopy with fluorescein diacetate being used to mark living parasites and the nucleic acid-binding compound ethidium bromide to stain dead cells [8].
  • After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids [9].

Chemical compound and disease context of ETHIDIUM BROMIDE


Biological context of ETHIDIUM BROMIDE


Anatomical context of ETHIDIUM BROMIDE

  • METHODS: NF-kappaB activation and IL-8 release in response to toxin A were correlated with reactive oxygen intermediate (ROI) generation and ATP production in HT-29 monolayers or HT-29 cells exposed to ethidium bromide (EB) to inhibit mitochondrial function [20].
  • First, unambiguous evidence was obtained that the DNA-binding dyes ethidium bromide (EtBr) and 4',6-diamidino-2-phenylindole (DAPI) exclusively stained mitochondria containing mtDNA in living human cells [21].
  • The chromosomal complements of mouse oocytes, ova, and fertilizing sperm have been studied by immunofluorescence with specific antisera to the basic protein fraction of sperm nuclei and to histones H2b and H4, and by staining with ethidium bromide [22].
  • For this purpose, we used cells with reduced mitochondrial DNA (mtDNA) contents by ethidium bromide treatment or myocytes treated with known mitochondrial metabolic inhibitors, including carbonyl cyanide m-chlorophenylhydrazone (CCCP), antimycin, valinomycin and azide [23].
  • In low salt medium, Ca2+ and EB were preferentially transported across the anodefacing cell membrane while PI and EthD-1 predominately entered at the site facing the cathode [24].

Associations of ETHIDIUM BROMIDE with other chemical compounds


Gene context of ETHIDIUM BROMIDE


Analytical, diagnostic and therapeutic context of ETHIDIUM BROMIDE


  1. Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus. Guntaka, R.V., Mahy, B.W., Bishop, J.M., Varmus, H.E. Nature (1975) [Pubmed]
  2. Torsional rigidity of positively and negatively supercoiled DNA. Selvin, P.R., Cook, D.N., Pon, N.G., Bauer, W.R., Klein, M.P., Hearst, J.E. Science (1992) [Pubmed]
  3. Effect of ethidium bromide on transplanted virus-induced tumor cells. Richardson, V.J., Vodinelich, L., Wilson, A., Potter, C.W. J. Natl. Cancer Inst. (1976) [Pubmed]
  4. Production of beta-bactamase by a strain of Neisseria gonorrhoeae when cultured in presence of ethidium bromide. Hafiz, S., Odugbemi, T.O., Geary, I., McEntegart, M.G. Lancet (1979) [Pubmed]
  5. A DNA assay protocol in a lab-on-valve meso-fluidic system with detection by laser-induced fluorescence. Chen, X., Wang, W., Wang, J. The Analyst. (2005) [Pubmed]
  6. Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. King, M.P., Attardi, G. Science (1989) [Pubmed]
  7. Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Giannini, S.H., Schittini, M., Keithly, J.S., Warburton, P.W., Cantor, C.R., Van der Ploeg, L.H. Science (1986) [Pubmed]
  8. Fluorogenic substrate detection of viable intracellular and extracellular pathogenic protozoa. Jackson, P.R., Pappas, M.G., Hansen, B.D. Science (1985) [Pubmed]
  9. Novel screening procedure for recombinant plasmids. Telford, J., Boseley, P., Schaffner, W., Birnstiel, M. Science (1977) [Pubmed]
  10. Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture. Contreras, J.E., Sánchez, H.A., Eugenin, E.A., Speidel, D., Theis, M., Willecke, K., Bukauskas, F.F., Bennett, M.V., Sáez, J.C. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  11. Correlation of ultrastructural and functional damage to mitochondria of ascites L1210 cells treated in vivo with methylglyoxal-bis(guanylhydrazone) or ethidium bromide. Porter, C.W., Mikles-Robertson, F., Kramer, D., Dave, C. Cancer Res. (1979) [Pubmed]
  12. Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. Forman, M.S., Nakamura, M., Mimori, T., Gelpi, C., Hardin, J.A. Arthritis Rheum. (1985) [Pubmed]
  13. Localization of the major ethidium bromide binding site on tRNA. Chu, W.C., Liu, J.C., Horowitz, J. Nucleic Acids Res. (1997) [Pubmed]
  14. Inhibition of c-Jun N-terminal kinase 1, but not c-Jun N-terminal kinase 2, suppresses apoptosis induced by ischemia/reoxygenation in rat cardiac myocytes. Hreniuk, D., Garay, M., Gaarde, W., Monia, B.P., McKay, R.A., Cioffi, C.L. Mol. Pharmacol. (2001) [Pubmed]
  15. DNA structure in sheared and unsheared chromatin. Nicolini, C., Baserga, R., Kendall, F. Science (1976) [Pubmed]
  16. Human fibroblast chromatin states as effectors of the DNA-binding characteristics of benzo[a]pyrene anti-7,8-dihydrodiol 9,10-epoxide and two nonalkylating DNA-binding molecules. Warner, M.R., Iannaccone, P., Fahl, W.E. J. Natl. Cancer Inst. (1986) [Pubmed]
  17. Diagnosis of beta-thalassaemia by DNA amplification in single blastomeres from mouse preimplantation embryos. Holding, C., Monk, M. Lancet (1989) [Pubmed]
  18. Detection and direct sequencing of hepatitis B virus genome by DNA amplification method. Yokosuka, O., Omata, M., Hosoda, K., Tada, M., Ehata, T., Ohto, M. Gastroenterology (1991) [Pubmed]
  19. Cytoplasmic inheritance of oligomycin resistance in Chinese hamster ovary cells. Breen, G.A., Scheffler, I.E. J. Cell Biol. (1980) [Pubmed]
  20. Clostridium difficile toxin A triggers human colonocyte IL-8 release via mitochondrial oxygen radical generation. He, D., Sougioultzis, S., Hagen, S., Liu, J., Keates, S., Keates, A.C., Pothoulakis, C., Lamont, J.T. Gastroenterology (2002) [Pubmed]
  21. Human mitochondria and mitochondrial genome function as a single dynamic cellular unit. Hayashi, J., Takemitsu, M., Goto, Y., Nonaka, I. J. Cell Biol. (1994) [Pubmed]
  22. Turnover of basic chromosomal proteins in fertilized eggs: a cytoimmunochemical study of events in vivo. Rodman, T.C., Pruslin, F.H., Hoffmann, H.P., Allfrey, V.G. J. Cell Biol. (1981) [Pubmed]
  23. Retrograde Ca2+ signaling in C2C12 skeletal myocytes in response to mitochondrial genetic and metabolic stress: a novel mode of inter-organelle crosstalk. Biswas, G., Adebanjo, O.A., Freedman, B.D., Anandatheerthavarada, H.K., Vijayasarathy, C., Zaidi, M., Kotlikoff, M., Avadhani, N.G. EMBO J. (1999) [Pubmed]
  24. Selective and asymmetric molecular transport across electroporated cell membranes. Tekle, E., Astumian, R.D., Chock, P.B. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  25. Crystalline ribosomes are present in brains from senile humans. O'Brien, L., Shelley, K., Towfighi, J., McPherson, A. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  26. Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction. Li, H., Cui, X., Arnheim, N. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  27. Mechanisms determining aerobic or anaerobic growth in the facultative anaerobe Salmonella typhimurium. Yamamoto, N., Droffner, M.L. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  28. Apparent role of traveling metabolic waves in oxidant release by living neutrophils. Kindzelskii, A.L., Petty, H.R. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  29. Ethidium bromide changes the nuclease-sensitive DNA binding sites of the antitumor drug cis-diamminedichloroplatinum(II). Tullius, T.D., Lippard, S.J. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  30. sir2 mutants of Kluyveromyces lactis are hypersensitive to DNA-targeting drugs. Chen, X.J., Clark-Walker, G.D. Mol. Cell. Biol. (1994) [Pubmed]
  31. Molecular study of eight Japanese cases of glucose-6-phosphate dehydrogenase deficiency by nonradioisotopic single-strand conformation polymorphism analysis. Hirono, A., Miwa, S., Fujii, H., Ishida, F., Yamada, K., Kubota, K. Blood (1994) [Pubmed]
  32. Novel PLA2G4C polymorphism as a molecular diagnostic assay for 19q loss in human gliomas. Hartmann, C., Johnk, L., Sasaki, H., Jenkins, R.B., Louis, D.N. Brain Pathol. (2002) [Pubmed]
  33. A rapid, non-radioactive screening test for fragile X mutations at the FRAXA and FRAXE loci. Wang, Q., Green, E., Bobrow, M., Mathew, C.G. J. Med. Genet. (1995) [Pubmed]
  34. Lack of beta 3-adrenergic receptor mRNA expression in adipose and other metabolic tissues in the adult human. Thomas, R.F., Liggett, S.B. Mol. Pharmacol. (1993) [Pubmed]
  35. Loss of reiterated DNA sequences during serial passage of human diploid fibroblasts. Shmookler Reis, R.J., Goldstein, S. Cell (1980) [Pubmed]
  36. Cooperative structural transition of PM2 DNA at high ionic strength and its dependence on DNA damages. Kuhnlein, U., Tsang, S.S., Edwards, J. Nature (1980) [Pubmed]
  37. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Sano, T., Smith, C.L., Cantor, C.R. Science (1992) [Pubmed]
  38. Nonisotopic SSCP detection in PCR products by ethidium bromide staining. Yap, E.P., McGee, J.O. Trends Genet. (1992) [Pubmed]
  39. DNA excision-repair defect of xeroderma pigmentosum prevents removal of a class of oxygen free radical-induced base lesions. Satoh, M.S., Jones, C.J., Wood, R.D., Lindahl, T. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
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