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Chemical Compound Review

Hydroethidine     5-ethyl-6-phenyl-6H- phenanthridine-3,8...

Synonyms: SureCN79004, D7008_SIAL, CTK8D4610, AR-1G6158, AKOS015915966, ...
 
 
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Disease relevance of ETHIDIUM BROMIDE

 

Psychiatry related information on ETHIDIUM BROMIDE

  • The experimental variables, i.e., pH of the buffer solution, the concentration and volume of EB solution, the reaction time and the fluid flow rates, were investigated [5].
 

High impact information on ETHIDIUM BROMIDE

  • Ethidium bromide reduces the formation of superhelical viral DNA and concurrently blocks integration of the viral genome [1].
  • Two human cell lines (termed rho 0), which had been completely depleted of mitochondrial DNA (mtDNA) by long-term exposure to ethidium bromide, were found to be dependent on uridine and pyruvate for growth because of the absence of a functional respiratory chain [6].
  • Among different geographic isolates of the same subspecies, more than two-thirds of chromosomes had similar sizes, ethidium bromide staining intensities, and locations of alpha,beta-tubulin genes [7].
  • Viable Leishmania promastigotes and amastigotes were detected by epifluorescence microscopy with fluorescein diacetate being used to mark living parasites and the nucleic acid-binding compound ethidium bromide to stain dead cells [8].
  • After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids [9].
 

Chemical compound and disease context of ETHIDIUM BROMIDE

 

Biological context of ETHIDIUM BROMIDE

 

Anatomical context of ETHIDIUM BROMIDE

  • METHODS: NF-kappaB activation and IL-8 release in response to toxin A were correlated with reactive oxygen intermediate (ROI) generation and ATP production in HT-29 monolayers or HT-29 cells exposed to ethidium bromide (EB) to inhibit mitochondrial function [20].
  • First, unambiguous evidence was obtained that the DNA-binding dyes ethidium bromide (EtBr) and 4',6-diamidino-2-phenylindole (DAPI) exclusively stained mitochondria containing mtDNA in living human cells [21].
  • The chromosomal complements of mouse oocytes, ova, and fertilizing sperm have been studied by immunofluorescence with specific antisera to the basic protein fraction of sperm nuclei and to histones H2b and H4, and by staining with ethidium bromide [22].
  • For this purpose, we used cells with reduced mitochondrial DNA (mtDNA) contents by ethidium bromide treatment or myocytes treated with known mitochondrial metabolic inhibitors, including carbonyl cyanide m-chlorophenylhydrazone (CCCP), antimycin, valinomycin and azide [23].
  • In low salt medium, Ca2+ and EB were preferentially transported across the anodefacing cell membrane while PI and EthD-1 predominately entered at the site facing the cathode [24].
 

Associations of ETHIDIUM BROMIDE with other chemical compounds

 

Gene context of ETHIDIUM BROMIDE

 

Analytical, diagnostic and therapeutic context of ETHIDIUM BROMIDE

References

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  2. Torsional rigidity of positively and negatively supercoiled DNA. Selvin, P.R., Cook, D.N., Pon, N.G., Bauer, W.R., Klein, M.P., Hearst, J.E. Science (1992) [Pubmed]
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