Disease relevance of flgD

• The order of these genes is identical to that of the corresponding region of the Treponema pallidum fla operon [1].
• These results showed that the T. denticola and T. pallidum fla operons are highly conserved, supporting the proposed phylogenetic relatedness of these spirochetes [1].
• Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12 [2].
• The strains with deletions starting within the prophage and ending in these fla genes were selected from among the heat-resistant survivors of the lysogen [2].
• The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322 [3].

High impact information on flgD

• We discuss the possibility that DNA-protein interactions at homologous nucleotide sequences like those identified in PII are part of a regulatory gene cascade that participates in timing fla gene expression in the C. crescentus cell cycle [4].
• Although fla genes are regulated at the transcriptional level [Ohta, N., Chen, L.-S., Swanson, E. & Newton, A. (1985) J. Mol. Biol. 186, 107-115], the 5' regulatory regions of C. crescentus genes have not been identified [4].
• Plasmid pJL1 was classified as a member of the FIV incompatibility group [5].
• To evaluate its role in the FIV cat model, we cloned and expressed feline IL-16 and determined its ability to induce chemotaxis as well as to inhibit FIV replication in cultured PBMCs [6].
• It was found that transcription of genes responsible for the formation and/or function of flagella (hag, fla, mot, che) decreased significantly at 40 degrees C. However, in the ftsI730 mutant at the nonpermissive temperature, or in penicillin G treated wild-type cells, cell division was blocked but formation of flagella continued [7].

Biological context of flgD

• Thirty of 99 Tp R-plasmids isolated from humans were grouped as Inc FIV and eight as Inc N [8].
• Restriction fragment length polymorphism analysis (RFLP) of fla A amplified product by using Bgl II enzyme classified 15 strains into 5 types [9].
• No analogs to the bacterial chemotactic and motility (che, mot, fla) genes, genes for a two-component signal transduction system, genes associated with gliding, or genomic homologs for the eukaryotic cytoskeleton and motor proteins were found in the Mollicutes [10].
• Incompatibility properties of the IncFIII/FIV haemolytic plasmid pSU316 when integrated in the Escherichia coli chromosome [11].

Analytical, diagnostic and therapeutic context of flgD

• The control group consisted of 30 FeLV-, FIV-, clinically healthy cats [12].
• The results of molecular weight determination of Tp R-plasmids performed by agarose gel electrophoresis were consistent with the existence of two groups--larger R-plasmids (76 to 104 Md) belonging to Inc FIV and lower molecular weight R-plasmids (25 to 35 Md) belonging to Inc N [8].
• Electron microscopy showed that flaW and flaV mutants carried hook-basal body structures [13].

References