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Gene Review

tdcB  -  L-threonine dehydratase, catabolic

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3106, JW3088
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Disease relevance of tdcB


High impact information on tdcB


Chemical compound and disease context of tdcB


Biological context of tdcB

  • Transcriptional analysis with S1 nuclease indicated two distinct transcription start sites upstream of the tdcB gene in regions previously identified as promoterlike elements P1 and P2 [7].
  • The tdc genes and ORFX showed similar codon usage and, in analogy with other bacterial genes, showed codon usage typical for genes expressed at an intermediate level [7].
  • The data suggest that P1, and not P2, is necessary for tdc gene expression, and that the DNA sequences coding for the 32 KD polypeptide and threonine dehydratase are part of a single transcriptional unit [8].
  • Deletion of the potential CAP-binding site from P1 prevented tdc gene expression [8].
  • A 936-base pair open reading frame was found between P1 and the tdc coding region, which produced a polypeptide of about 32 kilodaltons [8].

Associations of tdcB with chemical compounds

  • We studied the effects of introducing two different threonine dehydratase genes into this threonine-producing system to gauge their effects on isoleucine production [2].
  • One of the products encoded by the tdc operon, TdcE, has recently been shown to be a 2-keto acid formate-lyase (C. Hesslinger, S. A. Fairhurst, and G. Sawers, Mol. Microbiol. 27:477-492, 1998) that can accept pyruvate as an enzyme substrate [9].

Other interactions of tdcB


Analytical, diagnostic and therapeutic context of tdcB


  1. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins. Ganduri, Y.L., Sadda, S.R., Datta, M.W., Jambukeswaran, R.K., Datta, P. Mol. Gen. Genet. (1993) [Pubmed]
  2. Metabolic redirection of carbon flow toward isoleucine by expressing a catabolic threonine dehydratase in a threonine-overproducing Corynebacterium glutamicum. Guillouet, S., Rodal, A.A., An, G.H., Gorret, N., Lessard, P.A., Sinskey, A.J. Appl. Microbiol. Biotechnol. (2001) [Pubmed]
  3. A novel mechanism controls anaerobic and catabolite regulation of the Escherichia coli tdc operon. Sawers, G. Mol. Microbiol. (2001) [Pubmed]
  4. Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases. Datta, P., Goss, T.J., Omnaas, J.R., Patil, R.V. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  5. Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation. Selinger, D.W., Saxena, R.M., Cheung, K.J., Church, G.M., Rosenow, C. Genome Res. (2003) [Pubmed]
  6. Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors. Sumantran, V.N., Tranguch, A.J., Datta, P. FEMS Microbiol. Lett. (1989) [Pubmed]
  7. Molecular characterization of the tdc operon of Escherichia coli K-12. Goss, T.J., Schweizer, H.P., Datta, P. J. Bacteriol. (1988) [Pubmed]
  8. Molecular cloning and expression of the biodegradative threonine dehydratase gene (tdc) of Escherichia coli K12. Goss, T.J., Datta, P. Mol. Gen. Genet. (1985) [Pubmed]
  9. The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation. Sawers, G., Hesslinger, C., Muller, N., Kaiser, M. J. Bacteriol. (1998) [Pubmed]
  10. Functional analysis of the tdcABC promoter of Escherichia coli: roles of TdcA and TdcR. Hagewood, B.T., Ganduri, Y.L., Datta, P. J. Bacteriol. (1994) [Pubmed]
  11. Precise mapping of the rnpB gene encoding the RNA component of RNase P in Escherichia coli K-12. Komine, Y., Inokuchi, H. J. Bacteriol. (1991) [Pubmed]
  12. Genetic analysis of the tdcABC operon of Escherichia coli K-12. Schweizer, H.P., Datta, P. J. Bacteriol. (1988) [Pubmed]
  13. Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strain. Hirose, K., Fujita, M., Takeuchi, M., Yumoto, N., Tokushige, M., Kawata, Y. Biotechnol. Appl. Biochem. (1992) [Pubmed]
  14. Physical linkage and transcriptional orientation of the tdc operon on the Escherichia coli chromosome. Schweizer, H.P., Datta, P. Mol. Gen. Genet. (1991) [Pubmed]
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