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Gene Review

xylB  -  xylulokinase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3553, JW3536
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Disease relevance of xylB


High impact information on xylB


Chemical compound and disease context of xylB

  • The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping [9].
  • Positive selection is achieved by cloning DNA into the unique restriction sites of pLEK100, to disrupt xylB expression, transforming E. coli, and then plating transformants on xylitol medium [10].

Biological context of xylB

  • The identification and mapping of xyl gene promoters suggest that the xylA and xylB genes are organized as an operon having a single xylose inducible promoter preceding the xylA gene [11].
  • The xylB gene consists of a 1.551-bp open reading frame (ORF) encoding 517 amino acids [2].
  • The deduced amino acid sequence of the xylB gene product did not exhibit a high degree of identity with other xylan-degrading enzymes or glycosidases [2].

Other interactions of xylB

  • The relationship between changes in mRNA (cDNA) levels and changes in specific activities was verified for two genes (xylA and xylB) with good agreement [12].
  • Cloning of GI in homologous as well as heterologous hosts has been carried out, with the prime aim of overproducing the enzyme and deciphering the genetic organization of individual genes (xylA, xylB, and xylR) in the xyl operon of different microorganisms [13].

Analytical, diagnostic and therapeutic context of xylB

  • Subcloning and DNA restriction mapping allowed us to locate the xylA and xylB genes on a 1.6 kbp Bg/II fragment and a 2.6 kbp HindIII-Sa/I fragment, respectively [11].


  1. Cloning and expression of the genes for xylose isomerase and xylulokinase from Klebsiella pneumoniae 1033 in Escherichia coli K12. Feldmann, S.D., Sahm, H., Sprenger, G.A. Mol. Gen. Genet. (1992) [Pubmed]
  2. Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities. Utt, E.A., Eddy, C.K., Keshav, K.F., Ingram, L.O. Appl. Environ. Microbiol. (1991) [Pubmed]
  3. Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: a beta-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity. Lorenz, W.W., Wiegel, J. J. Bacteriol. (1997) [Pubmed]
  4. Selective cloning of Bacillus subtilis xylose isomerase and xylulokinase in Escherichia coli genes by IS5-mediated expression. Wilhelm, M., Hollenberg, C.P. EMBO J. (1984) [Pubmed]
  5. Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein. Hecht, S., Eisenreich, W., Adam, P., Amslinger, S., Kis, K., Bacher, A., Arigoni, D., Rohdich, F. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  6. Xylene monooxygenase catalyzes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101. Bühler, B., Schmid, A., Hauer, B., Witholt, B. J. Biol. Chem. (2000) [Pubmed]
  7. D-Xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Gene cloning, sequence and expression. Loviny-Anderton, T., Shaw, P.C., Shin, M.K., Hartley, B.S. Biochem. J. (1991) [Pubmed]
  8. Genetic organization and regulation of the xylose degradation genes in Streptomyces rubiginosus. Wong, H.C., Ting, Y., Lin, H.C., Reichert, F., Myambo, K., Watt, K.W., Toy, P.L., Drummond, R.J. J. Bacteriol. (1991) [Pubmed]
  9. Molecular cloning of TOL genes xylB and xylE in Escherichia coli. Inouye, S., Nakazawa, A., Nakazawa, T. J. Bacteriol. (1981) [Pubmed]
  10. A novel xylB-based positive selection vector. Stevis, P.E., Ho, N.W. Plasmid (1988) [Pubmed]
  11. Cloning and characterization of the xyl genes from Escherichia coli. Rosenfeld, S.A., Stevis, P.E., Ho, N.W. Mol. Gen. Genet. (1984) [Pubmed]
  12. Engineering a homo-ethanol pathway in Escherichia coli: increased glycolytic flux and levels of expression of glycolytic genes during xylose fermentation. Tao, H., Gonzalez, R., Martinez, A., Rodriguez, M., Ingram, L.O., Preston, J.F., Shanmugam, K.T. J. Bacteriol. (2001) [Pubmed]
  13. Molecular and industrial aspects of glucose isomerase. Bhosale, S.H., Rao, M.B., Deshpande, V.V. Microbiol. Rev. (1996) [Pubmed]
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