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Gene Review

xylR  -  xylose divergent operon transcriptional...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3558, JW3541
 
 
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Disease relevance of xylR

  • Southern hybridization performed with the Lactobacillus pentosus xylR gene (encoding the D-xylose repressor protein) as a probe revealed the existence of a xylR homologue in L. brevis which is not located with the xyABT locus [1].
  • The regulatory gene xylR of the TOL plasmid, which functions positively on both xylABC and xylDEGF operons in the presence of m-xylene or m-methylbenzyl alcohol, was cloned onto an Escherichia coli vector, pACYC177 [2].
  • Activation and repression of transcription at the double tandem divergent promoters for the xylR and xylS genes of the TOL plasmid of Pseudomonas putida [3].
 

High impact information on xylR

  • The xylABC operon on the TOL plasmid directs the synthesis of enzymes for conversion of toluene to benzoate and is positively controlled by the regulatory gene xylR [4].
  • The DNA was about 7.7 kb long and contained genes for a ribose binding protein and part of a ribose transporter, xylR (a putative regulatory gene), and the xyl operon, along with its regulatory region and transcription termination signal, in this order [5].
  • The restriction map and the gene order showed strong similarities with published maps of the DNA encoding both the entire meta pathway operon (xylDLEGFJIH) and the regulatory genes xylS and xylR on the archetype TOL plasmid pWW0, suggesting a high degree of conservation in DNA structure for the catabolic operon on the two different plasmids [6].
  • Secondary mutations abolishing the ribose-utilizing phenotype were obtained both in the xylFG genes coding for the xylose high-affinity transporter and in xylR that is required for the xyl gene expression [7].
  • Cloning of GI in homologous as well as heterologous hosts has been carried out, with the prime aim of overproducing the enzyme and deciphering the genetic organization of individual genes (xylA, xylB, and xylR) in the xyl operon of different microorganisms [8].
 

Biological context of xylR

  • Mutant derivatives of the TOL plasmid pWW0-161, containing Tn5 insertions in the xylS and xylR regulatory genes of the catabolic pathway, have been identified and characterized [9].
 

Associations of xylR with chemical compounds

  • Insertion of a kanamycin resistance gene into xylR encoding the xylose-dependent repressor leads to the loss of xylose-dependent regulation and to a small drop in the efficiency of glucose repression to eightfold [10].
 

Analytical, diagnostic and therapeutic context of xylR

References

  1. Molecular cloning and functional expression in lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis. Chaillou, S., Bor, Y.C., Batt, C.A., Postma, P.W., Pouwels, P.H. Appl. Environ. Microbiol. (1998) [Pubmed]
  2. Molecular cloning of regulatory gene xylR and operator-promoter regions of the xylABC and xylDEGF operons of the TOL plasmid. Inouye, S., Nakazawa, A., Nakazawa, T. J. Bacteriol. (1983) [Pubmed]
  3. Activation and repression of transcription at the double tandem divergent promoters for the xylR and xylS genes of the TOL plasmid of Pseudomonas putida. Marqués, S., Gallegos, M.T., Manzanera, M., Holtel, A., Timmis, K.N., Ramos, J.L. J. Bacteriol. (1998) [Pubmed]
  4. Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida. Inouye, S., Ebina, Y., Nakazawa, A., Nakazawa, T. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  5. Sequencing and characterization of the xyl operon of a gram-positive bacterium, Tetragenococcus halophila. Takeda, Y., Takase, K., Yamato, I., Abe, K. Appl. Environ. Microbiol. (1998) [Pubmed]
  6. Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53. Keil, H., Keil, S., Pickup, R.W., Williams, P.A. J. Bacteriol. (1985) [Pubmed]
  7. Utilization of D-ribose through D-xylose transporter. Song, S., Park, C. FEMS Microbiol. Lett. (1998) [Pubmed]
  8. Molecular and industrial aspects of glucose isomerase. Bhosale, S.H., Rao, M.B., Deshpande, V.V. Microbiol. Rev. (1996) [Pubmed]
  9. Localization and functional analysis of transposon mutations in regulatory genes of the TOL catabolic pathway. Franklin, F.C., Lehrbach, P.R., Lurz, R., Rueckert, B., Bagdasarian, M., Timmis, K.N. J. Bacteriol. (1983) [Pubmed]
  10. Catabolite repression of the xyl operon in Bacillus megaterium. Rygus, T., Hillen, W. J. Bacteriol. (1992) [Pubmed]
  11. Monitoring aromatic hydrocarbons by whole cell electrochemical biosensors. Paitan, Y., Biran, I., Shechter, N., Biran, D., Rishpon, J., Ron, E.Z. Anal. Biochem. (2004) [Pubmed]
 
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