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Gene Review

pheA  -  bifunctional chorismate mutase/prephenate...

Escherichia coli O157:H7 str. EDL933

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Disease relevance of pheA


High impact information on pheA

  • The presence of two different mechanisms for de-attenuation is further indicated by the finding that a second attenuator controlled by Phe codon translation, from the pheA operon, is affected quite differently by the mutant tRNAs [5].
  • We have cloned and sequenced a region of the genome of B. aphidicola isolated from Acyrthosiphon pisum which includes the two-domain aroQ/pheA gene [6].
  • Following its localization on the 11-kb B. stearothermophilus DNA insert by deletion and expression analysis, the phenol hydroxylase gene pheA was subcloned as a 2-kb HindIII fragment, whose transformant expressed the enzyme after phenol induction and even more strongly after o-, m-, and p-cresol induction [4].
  • We show that expression of the Escherichia coli pheA gene, inserted into the pPS10 vector downstream from Pba, increases about 70-fold upon InGP accumulation [7].
  • The instability of a recombinant plasmid carrying a pheA insert cloned from Erwinia herbicola resulted in a loss of 260 bp plus the TAA stop codon from the 3' terminus of pheA [2].

Chemical compound and disease context of pheA


Biological context of pheA

  • The gene trpX codes for an enzyme that modifies both tRNATrp and tRNAPhe and a mutation in that gene causes derepression of the trp and pheA operons, both of which are controlled by attenuation [11].
  • The plasmid pEST1412 contains the genes, pheA and pheB, encoding phenol monooxygenase (PMO) and catechol 1,2-dioxygenase (C12]), respectively [3].
  • Comparison of the deduced amino acid sequences of tfdB and pheA revealed highly conserved regions in the protein products of these genes [3].
  • The nucleotide sequence of the 2.0-kb PstI-HindIII fragment of pEST1412 carrying pheA was determined [3].
  • The gene order for the cys A region was determined to be as follows: pheA ... purC-cys A-trz A-pts-dsd-aro D-purF ... his, and special mapping problems for this region are discussed [12].

Anatomical context of pheA

  • These results demonstrate that efficient translation of the pheA leader peptide coding region and the position of the ribosome on the leader transcript play decisive roles in the attenuation regulation of pheA [1].

Associations of pheA with chemical compounds


Analytical, diagnostic and therapeutic context of pheA

  • As a preliminary to sequence analysis, a small DNA insertion at codon 338 of the pheA gene unexpectedly resulted in a partial loss of prephenate dehydratase feedback inhibition [10].


  1. Role of translation of the pheA leader peptide coding region in attenuation regulation of the Escherichia coli pheA gene. Gavini, N., Pulakat, L. J. Bacteriol. (1991) [Pubmed]
  2. Loss of allosteric control but retention of the bifunctional catalytic competence of a fusion protein formed by excision of 260 base pairs from the 3' terminus of pheA from Erwinia herbicola. Xia, T., Zhao, G., Jensen, R.A. Appl. Environ. Microbiol. (1992) [Pubmed]
  3. Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas sp. EST1001: expression in Escherichia coli and Pseudomonas putida. Nurk, A., Kasak, L., Kivisaar, M. Gene (1991) [Pubmed]
  4. Characterization of the Bacillus stearothermophilus BR219 phenol hydroxylase gene. Kim, I.C., Oriel, P.J. Appl. Environ. Microbiol. (1995) [Pubmed]
  5. Mutants of pheV in Escherichia coli affecting control by attenuation of the pheS, T and pheA operons. Two distinct mechanisms for de-attenuation. Pages, D., Buckingham, R.H. J. Mol. Biol. (1990) [Pubmed]
  6. Prephenate dehydratase from the aphid endosymbiont (Buchnera) displays changes in the regulatory domain that suggest its desensitization to inhibition by phenylalanine. Jiménez, N., González-Candelas, F., Silva, F.J. J. Bacteriol. (2000) [Pubmed]
  7. Controlled-expression shuttle vector for pseudomonads based on the trpIBA genes of Pseudomonas putida. Olekhnovich, I.N., Fomichev, Y.K. Gene (1994) [Pubmed]
  8. A novel phenol hydroxylase and catechol 2,3-dioxygenase from the thermophilic Bacillus thermoleovorans strain A2: nucleotide sequence and analysis of the genes. Duffner, F.M., Müller, R. FEMS Microbiol. Lett. (1998) [Pubmed]
  9. Cloning, sequencing, and expression of the P-protein gene (pheA) of Pseudomonas stutzeri in Escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis. Fischer, R.S., Zhao, G., Jensen, R.A. J. Gen. Microbiol. (1991) [Pubmed]
  10. Novel mutations in the pheA gene of Escherichia coli K-12 which result in highly feedback inhibition-resistant variants of chorismate mutase/prephenate dehydratase. Nelms, J., Edwards, R.M., Warwick, J., Fotheringham, I. Appl. Environ. Microbiol. (1992) [Pubmed]
  11. Escherichia coli phenylalanyl-tRNA synthetase operon is controlled by attenuation in vivo. Springer, M., Trudel, M., Graffe, M., Plumbridge, J., Fayat, G., Mayaux, J.F., Sacerdot, C., Blanquet, S., Grunberg-Manago, M. J. Mol. Biol. (1983) [Pubmed]
  12. Transduction by phage P1CM clr-100 in Salmonella typhimurium. Mojica, T. Mol. Gen. Genet. (1975) [Pubmed]
  13. Effect of L-serine on the biosynthesis of aromatic amino acids in Escherichia coli. Tazuya-Murayama, K., Aramaki, H., Mishima, M., Saito, K., Ishida, S., Yamada, K. J. Nutr. Sci. Vitaminol. (2006) [Pubmed]
  14. Defective regulation of the phenylalanine biosynthetic operon in mutants of the phenylalanyl-tRNA synthetase operon. Borg-Olivier, S.A., Tarlinton, D., Brown, K.D. J. Bacteriol. (1987) [Pubmed]
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