Disease relevance of p-fluorophenylalanine

• In an attempt to detect the phenylalanine (Phe) binding site, two Escherichia coli PheRS mutant strains (pheS), which were resistant to p-fluorophenylalanine (p-F-Phe) were analysed genetically [1].
• Appearance of straight flagellar filaments in the presence of p-fluorophenylalanine in Pseudomonas aeruginosa [2].
• This phage transduces with high frequency (i) several temperature-sensitive PRS mutants to thermoresistance and (ii) a p-fluorophenylalanine resistant PRS mutant to sensitivity against this amino-acid analog [3].
• Protein synthesis inhibitor transiently reduces neuronal death in the thalamus of spontaneously hypertensive rats following cortical infarction [4].
• Protein synthesis inhibitor-mediated stability of adenomatous polyposis coli mRNA levels in HCT-116 colon cancer cells [5].

High impact information on p-fluorophenylalanine

• UV mutagenesis of the al-3p-mtr(+)-carrying strain allowed us to isolate two mutant strains, BLR-1 and BLR-2 (blue light regulator), that are light-resistant to p-fluorophenylalanine and have lost the ability to grow on tryptophan [6].
• The equilibrium unfolding behavior of the intestinal fatty acid-binding protein has been investigated by (19)F-NMR after incorporation of 4-fluorophenylalanine and by pulsed field gradient diffusion (1)H-NMR [7].
• HierDock identifies p-fluorophenylalanine and 3-thienylalanine as especially good substrates for PheRS, in agreement with experiment [8].
• Recovery of HeLa cells from inhibited entry into mitosis induced by p-fluorophenylalanine [9].
• Fluorine nuclear magnetic resonance studies of the cleavage of peptides containing a 4-fluorophenylalanine (FPhe)-Pro bond have been performed in order to determine the conformational specificity of FPhe-Pro bond cleavage by pepsin [10].

Chemical compound and disease context of p-fluorophenylalanine

• Effects of p-fluorophenylalanine on the induction of mutations in bacteriophage T4. II. Nitrous acid mutagenesis [11].

Biological context of p-fluorophenylalanine

• The analogue amino acid, p-fluorophenylalanine also showed immediate linear kinetics of incorporation [12].
• Effects of the incorporation of p-fluorophenylalanine on the in vitro lifespan of human diploid cells [13].
• A plasmid bearing wild-type DNA from this region restored p-fluorophenylalanine sensitivity and wild-type repression of the pheA operon [14].
• Among mutants of Escherichia coli resistant to p-fluorophenylalanine (PFP) were some with constitutive expression of the phenylalanine biosynthetic operon (the pheA operon) [14].
• In A. nidulans, we did not observe any increase in the frequency of recessive homozygous fpaA/fpaA (p-fluorophenylalanine-resistant) mutants over the expected one, which has been calculated on the basis of the observed mutation frequency in the haploid strain [15].

Anatomical context of p-fluorophenylalanine

• The effect of p-fluorophenylalanine incorporation on PGI was examined in early passage fibroblasts [16].
• In the presence of p-fluorophenylalanine, a normally flagellated strain of Pseudomonas aeruginosa produced straight flagellar filaments at the distal ends of preexisting flagella, indicating polar growth on its flagella [2].
• Protein synthesis inhibitor, cycloheximide, blocked oocyte maturation at germinal vesicle breakdown(GVBD), if added to the maturation medium between 0 to 4 h, suggesting that protein synthesis is required for GVBD [17].
• One p-fluorophenylalanine-resistant cell line shows greatly reduced uptake of all amino acids so is resistant to growth inhibition by other amino acid analogs [18].

Associations of p-fluorophenylalanine with other chemical compounds

• Pretreatment of rats with the protein syNthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 microM) [19].
• A new plasmid cloning vector (pHE3) is described carrying dominant p-fluorophenylalanine-sensitivity (pheS) and chloramphenicol-resistance markers [20].
• In this study, a double transfer method involving only brief exposure to p-fluorophenylalanine (FPA) allowed increased recovery of auxotrophic markers from heterozygous diploids [21].
• Shikonin production by p-fluorophenylalanine resistant cells of Lithospermum erythrorhizon [22].
• Adenosine triphosphatases in p-fluorophenylalanine sensitive and resistant strains of Aspergillus nidulans [23].

Gene context of p-fluorophenylalanine

• Protein synthesis inhibitor cycloheximide does not block 1alpha,25(OH)2D3 mediated upregulation of CH25H mRNA [24].
• Previous studies have shown that the amino acid analogue p-fluorophenylalanine (PFP) is mutagenic to Coprinus lagopus due to its incorporation into proteins [32] [25].
• Protein synthesis inhibitor and lysosomotropic agents did not alter the arginase level [26].

References