Disease relevance of Ochromonas

• Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO) [1].

High impact information on Ochromonas

• The phylogenetic relationships among the chlorophyte Chlamydomonas reinhardtii, the chrysophyte Ochromonas danica, and the oomycete Achyla bisexualis were explored by comparing the sequences of their small-subunit ribosomal RNA coding regions [2].
• 5-S-Ethyl-5-thio-D-ribose (ethylthioribose) exhibits antiprotozoal activity against Plasmodium falciparum, Giardia lamblia, and Ochromonas malhamensis, but is nontoxic to cultured human and murine bone marrow cells (Riscoe, M [3].
• Ethidium bromide has no effect on chloroplast ultrastructure in Ochromonas [4].
• Conversely, when cells of Ochromonas are treated with cycloheximide, there is a reduction in the amount of periplastidal reticulum presumably because there are no cytoplasmically synthesized proteins to be transported into the chloroplast [5].
• In the rat, chronic alcohol feeding for up to 6 months had no effects on tissue thiamin measured by transketolase assay, thiamin pyrophosphate effect, or bioassay with Ochromonas danica [6].

Anatomical context of Ochromonas

• Experimental evidence for this hypothesis comes from studies on Ochromonas danica using chloramphenicol and spectinomycin, which inhibit protein synthesis on plastid ribosomes, and cycloheximide, which inhibits protein synthesis on cytoplasmic ribosomes [5].

Associations of Ochromonas with chemical compounds

• The large subunit ribosomal RNA sequences from the heterokont algae Ochromonas danica, Nannochloropsis salina, and Tribonema aequale were determined [7].
• A polar diacylglycerolipid isolated from the phototrophic green alga Chlamydomonas reinhardtii 137+ (wild-type) displays chromatographic and chemical identity with the either-linked homoserine lipid 1(3),2-diacylglyceryl-(3)-O-4'(N,N,N-trimethyl)homoserine (DGTS) of the golden alga Ochromonas danica [8].
• In the red alga Cyanidium caldarium and in the Chrysophyte Ochromonas danica a clear dichotomy was observed: as in higher plants, sterols were formed via the MVA route, whereas chloroplast isoprenoids (phytol in Cy. caldarium and O. danica and beta-carotene in O. danica) were synthesized via the GAP/pyruvate route [9].
• The eukaryotic alga Ochromonas danica, a nutritionally versatile, mixotrophic chrysophyte, grew on phenol as the sole carbon source in axenic culture and removed the phenol carbon from the growth medium [10].
• Studies on metabolic role of 5'-Methylthioadenosine in Ochromonas malhamensis and other microorganisms [11].

Gene context of Ochromonas

• Adult Hymenolepis diminuta did not take up CN-Cbl in vitro, which correlated with the finding that no CN-Cbl was detected in the worm by Ochromonas malhamensis assay [12].
• Since the transition value of the modulus of elasticity is about 5 dynes/mum2 (or stiffness of 3.5 X 10(-16) dyne cm2) for the mastigonemes of Ochromonas it would appear that the actual value must be in excess of this [13].

References