Disease relevance of Transfectam

• When using the cationic lipid dioctadecylamido glycylspermine (Transfectam, DOGS) with a cytomegalovirus (CMV)-luciferase reporter gene, the best levels of transfection were obtained when using a low ratio of positive charges (supplied by the DOGS) to negative charges (carried by the DNA) [1].
• In addition, gene transfer efficiency of DNA complexes with lower amounts of Transfectam (1.5-2 charge equiv) is increased by a factor of up to 1000 by peptides INF6 (influenza virus derived sequence) and INF10 (artificial sequence) [2].
• A previous study showed that filamentous phage could be efficiently transfected into mammalian cells in the presence of the cationic lipid Transfectam [3].
• 1. Dogs were exposed to hypoxemia followed by a coronary angiogram at three different times: under control conditions, after ischemia-reperfusion injury, then 30 min later [4].
• This vector was employed to transduce primary rat skeletal myoblasts in optimised conditions involving a concomitant use of the retrovirus immobilising protein RetroNectin and the polycation transduction enhancer Transfectam [5].

High impact information on Transfectam

• We therefore investigated to what extent the DNA condensation buffer influences the transfection efficiency of Transfectam/DNA particles [6].
• Our results show that in a variety of cell lines, a greater than 100-fold difference in luciferase gene expression is observed with Transfectam/DNA complexes at a +/- charge ratio of 0.75 depending on the conditions of complex formation [6].
• Dioctadecylamidoglycylspermine (DOGS, Transfectam) is a cationic lipid able to interact with DNA to form complexes that mediate efficient gene transfer into various eukaryotic cells [6].
• The conventional and fluorinated lipoplexes were formulated from Transfectam" (or DOGS) and from fluorinated lipospermines, analogs of DOGS, respectively, and a luciferase reporter plasmid [7].
• 1. Dogs with bilateral auditory cortex lesions were tested on their ability to localize and discriminate brief sounds [8].

Biological context of Transfectam

• 1. Dogs cooled to 27 degrees C were compared with control dogs maintained at 38 degrees C. The mean arterial blood pressure, renal blood flow and glomerular filtration rate were lower in the hypothermic animals [9].

Anatomical context of Transfectam

• The rAR-containing expression vector was transiently transfected into COS-1 cells using Transfectam Reagent, in order to achieve high transfection efficiency [10].
• The method employs small amounts of the cationic lipid Transfectam (DOGS), in a concentrated (40 mM) ethanolic solution, to compact and to transfer exogenous genes into chick embryos during the early stages of development (< 36 h of incubation) [11].
• 1. Dogs with bile-duct ligation retain salt and water and form ascites [12].

Associations of Transfectam with other chemical compounds

• Transfection efficiency was studied in several primary skin cells (FEK4, FCP4, FCP5, FCP7, and FCP8) and in an immortalized cancer cell line (HtTA) and was compared with the commercially available nonliposomal transfection formulation Transfectam (dioctadecylamidoglycyl spermine), which also contains two saturated C18 lipid chains [13].
• Transfection efficiency of four liposomes: Transfectam, Lipofectin, Unifectin-10, and Maxifectin was compared [14].
• For the in vitro transfection assays, the lipoplexes and polyplexes were formulated from the preformed Acr-spacer-NLS/DNA complexes and dioctadecylamidoglycylspermine (DOGS)/dioleylphosphatidylethanolamine (DOPE) 1:1 mol and branched polyethyleneimine (PEI) 25 kDa, respectively, which are very efficient in vitro gene transfer systems [15].

Analytical, diagnostic and therapeutic context of Transfectam

• Transfectam and PEI (polyethylenimine)-mediated transfection also improved after mild centrifugation of the complexes on to the cells [16].
• 1. Dogs absorbed [14C]nadolol almost completely (88--104%) after oral administration of 10--75 mg/kg [17].

References