The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

Lipofectin     2,3-bis[[(E)-octadec-9- enoyl]oxy]propoxy...

Synonyms: AC1Q2VXI, AC1O5MZ2, 1,2-Dope, 76391-83-8, Dioleoyl cephalin, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Lipofectin


High impact information on Lipofectin

  • The results of this study were consistent with previously published results indicating that only low levels of luciferase gene expression could be obtained by Lipofectin-mediated gene transfer [6].
  • CONCLUSIONS--The recombinant adenoviral vectors proved to be far more effective than Lipofectin at delivering foreign genes directly into the coronary arteries of living mammals [6].
  • To investigate the role of gap junctions in the tumor characteristics of these cells, we have used Lipofectin-mediated transfection to introduce a full-length cDNA encoding connexin 43 [7].
  • Using the RNA/lipofectin transfection procedure, we have analyzed the role of capping and beta-globin 5' and 3' untranslated sequences on the translation efficiency of luciferase RNA synthesized in vitro [8].
  • The elimination of mRNA, protein, and JNK activities lasted 48 and 72 h following a single Lipofectin treatment with antisense JNK1 and JNK2, respectively, indicating sufficient duration for examining the impact of specific elimination on the phenotype [9].

Chemical compound and disease context of Lipofectin

  • Ecotropic murine leukemia virus and packaged retroviral vectors were shown to infect mink cells, and amphotropic packaged retroviral vectors were shown to infect hamster cells in the presence of Lipofectin but not in the presence of Polybrene [5].
  • To evaluate the influence of cell type and cationic liposomal formulation on gene transfection efficiency three liposomes (lipofectin, lipofectamine and DOTAP) were used to transfect the human (A172 and MOG-G-CCM) and rodent (C6 and A15A5) glioma cell lines with the Lac Z gene [10].
  • We conclude that Cytofectin GSV is superior to the other transfection reagents, predominantly at haEC, showing an improved efficacy and less toxicity than the classical liposome Lipofectin [11].
  • We used commercially available cationic liposomes, lipofectin, DOTAP, and transfectam, to enhance the antiherpetic activities of phosphodiester oligonucleotides (D-oligos) or phosphorothioate oligonucleotides (S-oligos) targeted against immediate-early pre-mRNA4/5 of herpes simplex virus type 1 (HSV-1) [12].

Biological context of Lipofectin

  • The peptoid condenses plasmid DNA into uniform particles 50-100 nm in diameter and mediates the transfection of a number of cell lines with efficiencies greater than or comparable to DMRIE-C, Lipofectin, and Lipofectamine [13].
  • To examine the feasibility of this strategy, pActLacZ, an expression vector composed of the LacZ gene driven by the beta-actin promoter, complexed with lipofectin, was injected retrogradely into the common bile ducts of adult rats [14].
  • A bicistronic mammalian expression vector containing a reporter gene (beta-galactosidase) and human IL-2 cDNA was complexed with either lipofectin or DC-cholesterol liposomes and transferred to tumor xenografts by direct intratumoral injection [15].
  • However, G3139 (when complexed with Lipofectin) did not induce the up-regulation of expression of either type I or type II IFNs, nor could IFNs be found in conditioned media from treated cells [16].
  • Lipofectamine, Cellfectin, and RPR120535b, but not Lipofectin, Lipofectace, or DOTAP, markedly improved transgene expression in CD34+ cells (from 19 to 35%) [17].

Anatomical context of Lipofectin

  • Using the reporter gene vectors pCAT and pCMV beta-gal, we have investigated the efficiency of transfection mediated by calcium phosphate precipitation, the monocationic liposome Lipofectin, the polycationic liposome Lipofectamine, and adenovirus-polylysine (AdpL) DNA complexes in human, mouse, rat, and fetal porcine islet cells [18].
  • Lipofectin transfection with 200 to 400 nM Ki-Ras oligo inhibited the epidermal growth factor- and fibroblast growth factor-stimulated proliferation of vero cells by 25 to 35% with a lesser effect on serum-stimulated growth [19].
  • For hepatocytes fully adapted to culture (approximately 48 hours after plating), pure DOTMA and Lipofectin were similarly effective [20].
  • Wild-type and G1144A mutant-type TNSALP cDNA expression vector pcDNA3 have been constructed and transfected to COS-1 cells by lipofectin technique [21].
  • Fractions of 100-1000 kb were ligated to Not I-digested vector arms and transformed into S.pombe protoplasts in the presence of lipofectin [22].

Associations of Lipofectin with other chemical compounds

  • We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into a liposome (lipofectin) [8].
  • We now report that lipofectin (L) incorporated into the ID complexes enhances integrin-mediated transfection, increasing luciferase expression by more than 100-fold [23].
  • The monovalent cationic liposomal formulations (DC-Chol and lipofectin) exhibited increased transfection activities comparable to that seen with the multivalent cationic liposome formulation, lipofectamine [24].
  • Although the degree of the polybrene effect depends on the nature of the cell line, these results indicate that individually optimized concentrations of polybrene can be useful for increasing the efficiency of lipofectin-mediated gene transfer in vitro [25].
  • The most active compound DMPE-(Nae-Nmpe-Nmpe)3 (Nae, N-aminoethyl glycine; Nmpe, N-p-methoxyphenethyl-glycine) is more efficient than lipofectin or DMRIE-C (two commercial cationic lipid transfection reagents) and is active in the presence and absence of serum [26].

Gene context of Lipofectin

  • However, treatment of C8161 with antisense 5-lipoxygenase (5-LO) oligonucleotides inhibits metastases 39% in Lipofectin-treated cells, but does not inhibit TNF-alpha-induced upregulation of experimental metastases [27].
  • HMC and MMC were lipofectin transfected with ras-targeted AS-oligo at 200-400 nM for 18 h followed by growth of cells in 20% serum for 18-72 h [28].
  • Accordingly, full-length LCAT cDNA was cloned into pVL 1392, a high-level expression derivative of Autographa californica nuclear polyhedrosis virus (AcNPV), and the resultant plasmid was co-transfected into Trichoplusia ni insect cells (High 5 line) with a linearized viral DNA using lipofectin [29].
  • Heterologous Chinese hamster ovary (CHO-K1) and human embryonic kidney 293 (HEK) cells were transfected with SSTR2 cDNA using lipofectin [30].
  • PTEN gene packaged with lipofectin was transferred into breast cancer cell line MDA468 and parental MDA468 cells served as controls [31].

Analytical, diagnostic and therapeutic context of Lipofectin


  1. Liposome-mediated transfection of intact viral particles reveals that plasma membrane penetration determines permissivity of tissue culture cells to rotavirus. Bass, D.M., Baylor, M.R., Chen, C., Mackow, E.M., Bremont, M., Greenberg, H.B. J. Clin. Invest. (1992) [Pubmed]
  2. Transfection of the human heme oxygenase gene into rabbit coronary microvessel endothelial cells: protective effect against heme and hemoglobin toxicity. Abraham, N.G., Lavrovsky, Y., Schwartzman, M.L., Stoltz, R.A., Levere, R.D., Gerritsen, M.E., Shibahara, S., Kappas, A. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  3. Temporary ex vivo inhibition of the expression of the human oncogene HER2 (NEU) by a triple helix-forming oligonucleotide. Porumb, H., Gousset, H., Letellier, R., Salle, V., Briane, D., Vassy, J., Amor-Gueret, M., Israël, L., Taillandier, E. Cancer Res. (1996) [Pubmed]
  4. Cationic polymer and lipids augment adenovirus-mediated gene transfer to cerebral arteries in vivo. Toyoda, K., Nakane, H., Heistad, D.D. J. Cereb. Blood Flow Metab. (2001) [Pubmed]
  5. Cationic liposomes (Lipofectin) mediate retroviral infection in the absence of specific receptors. Innes, C.L., Smith, P.B., Langenbach, R., Tindall, K.R., Boone, L.R. J. Virol. (1990) [Pubmed]
  6. Percutaneous transluminal in vivo gene transfer by recombinant adenovirus in normal porcine coronary arteries, atherosclerotic arteries, and two models of coronary restenosis. French, B.A., Mazur, W., Ali, N.M., Geske, R.S., Finnigan, J.P., Rodgers, G.P., Roberts, R., Raizner, A.E. Circulation (1994) [Pubmed]
  7. Transfection of C6 glioma cells with connexin 43 cDNA: analysis of expression, intercellular coupling, and cell proliferation. Zhu, D., Caveney, S., Kidder, G.M., Naus, C.C. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  8. Cationic liposome-mediated RNA transfection. Malone, R.W., Felgner, P.L., Verma, I.M. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  9. The Jun kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. Bost, F., McKay, R., Bost, M., Potapova, O., Dean, N.M., Mercola, D. Mol. Cell. Biol. (1999) [Pubmed]
  10. Liposomal transfection efficiency and toxicity on glioma cell lines: in vitro and in vivo studies. Bell, H., Kimber, W.L., Li, M., Whittle, I.R. Neuroreport (1998) [Pubmed]
  11. Toxicity, uptake kinetics and efficacy of new transfection reagents: increase of oligonucleotide uptake. Axel, D.I., Spyridopoulos, I., Riessen, R., Runge, H., Viebahn, R., Karsch, K.R. J. Vasc. Res. (2000) [Pubmed]
  12. Limited use of cationic liposomes as tools to enhance the antiherpetic activities of oligonucleotides in vero cells infected with herpes simplex virus type 1. Shoji, Y., Norimatsu, M., Shimada, J., Mizushima, Y. Antisense Nucleic Acid Drug Dev. (1998) [Pubmed]
  13. A combinatorial approach to the discovery of efficient cationic peptoid reagents for gene delivery. Murphy, J.E., Uno, T., Hamer, J.D., Cohen, F.E., Dwarki, V., Zuckermann, R.N. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  14. Expression of the hepatitis C virus genome in rat liver after cationic liposome-mediated in vivo gene transfer. Takehara, T., Hayashi, N., Miyamoto, Y., Yamamoto, M., Mita, E., Fusamoto, H., Kamada, T. Hepatology (1995) [Pubmed]
  15. In vivo cytokine gene therapy of human tumor xenografts in SCID mice by liposome-mediated DNA delivery. Egilmez, N.K., Cuenca, R., Yokota, S.J., Sorgi, F., Bankert, R.B. Gene Ther. (1996) [Pubmed]
  16. Changes in gene expression induced by phosphorothioate oligodeoxynucleotides (including G3139) in PC3 prostate carcinoma cells are recapitulated at least in part by treatment with interferon-beta and -gamma. Benimetskaya, L., Wittenberger, T., Stein, C.A., Hofmann, H.P., Weller, C., Lai, J.C., Miller, P., Gekeler, V. Clin. Cancer Res. (2004) [Pubmed]
  17. Lipofectamine and related cationic lipids strongly improve adenoviral infection efficiency of primitive human hematopoietic cells. Byk, T., Haddada, H., Vainchenker, W., Louache, F. Hum. Gene Ther. (1998) [Pubmed]
  18. Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes. Saldeen, J., Curiel, D.T., Eizirik, D.L., Andersson, A., Strandell, E., Buschard, K., Welsh, N. Diabetes (1996) [Pubmed]
  19. Evidence of a role for Ki-Ras in the stimulated proliferation of renal fibroblasts. Sharpe, C.C., Dockrell, M.E., Scott, R., Noor, M.I., Cowsert, L.M., Monia, B.P., Hendry, B.M. J. Am. Soc. Nephrol. (1999) [Pubmed]
  20. Cationic lipid-mediated transfection of liver cells in primary culture. Jarnagin, W.R., Debs, R.J., Wang, S.S., Bissell, D.M. Nucleic Acids Res. (1992) [Pubmed]
  21. Function of mutant (G1144A) tissue-nonspecific ALP gene from hypophosphatasia. Watanabe, H., Goseki-Sone, M., Orimo, H., Hamatani, R., Takinami, H., Ishikawa, I. J. Bone Miner. Res. (2002) [Pubmed]
  22. A Schizosaccharomyces pombe artificial chromosome large DNA cloning system. Young, D.J., Nimmo, E.R., Allshire, R.C. Nucleic Acids Res. (1998) [Pubmed]
  23. Lipid-mediated enhancement of transfection by a nonviral integrin-targeting vector. Hart, S.L., Arancibia-Cárcamo, C.V., Wolfert, M.A., Mailhos, C., O'Reilly, N.J., Ali, R.R., Coutelle, C., George, A.J., Harbottle, R.P., Knight, A.M., Larkin, D.F., Levinsky, R.J., Seymour, L.W., Thrasher, A.J., Kinnon, C. Hum. Gene Ther. (1998) [Pubmed]
  24. Protamine sulfate enhances lipid-mediated gene transfer. Sorgi, F.L., Bhattacharya, S., Huang, L. Gene Ther. (1997) [Pubmed]
  25. Polybrene increases the efficiency of gene transfer by lipofection. Abe, A., Miyanohara, A., Friedmann, T. Gene Ther. (1998) [Pubmed]
  26. Lipitoids--novel cationic lipids for cellular delivery of plasmid DNA in vitro. Huang, C.Y., Uno, T., Murphy, J.E., Lee, S., Hamer, J.D., Escobedo, J.A., Cohen, F.E., Radhakrishnan, R., Dwarki, V., Zuckermann, R.N. Chem. Biol. (1998) [Pubmed]
  27. Enhanced metastatic ability of TNF-alpha-treated malignant melanoma cells is reduced by intercellular adhesion molecule-1 (ICAM-1, CD54) antisense oligonucleotides. Miele, M.E., Bennett, C.F., Miller, B.E., Welch, D.R. Exp. Cell Res. (1994) [Pubmed]
  28. Distinct functions for Ras GTPases in the control of proliferation and apoptosis in mouse and human mesangial cells. Hendry, B.M., Khwaja, A., Qu, Q.Y., Shankland, S.J. Kidney Int. (2006) [Pubmed]
  29. Secretion of active human lecithin-cholesterol acyltransferase by insect cells infected with a recombinant baculovirus. Chawla, D., Owen, J.S. Biochem. J. (1995) [Pubmed]
  30. Activation of human somatostatin receptor type 2 causes inhibition of cell growth in transfected HEK293 but not in transfected CHO cells. Ren, J., Bell, G., Coy, D.H., Brunicardi, F.C. J. Surg. Res. (1997) [Pubmed]
  31. Exogenous PTEN gene induces apoptosis in breast carcinoma cell line MDA468. Chen, Q., Wang, C., Jiang, C., Chen, D. J. Huazhong Univ. Sci. Technol. Med. Sci. (2007) [Pubmed]
  32. Application to vascular adventitia of a nonviral vector for TIMP-1 gene therapy to prevent intimal hyperplasia. Meng, Q.H., Jamal, W., Hart, S.L., McEwan, J.R. Hum. Gene Ther. (2006) [Pubmed]
  33. Cationic lipid is not required for uptake and selective inhibitory activity of ICAM-1 phosphorothioate antisense oligonucleotides in keratinocytes. Nestle, F.O., Mitra, R.S., Bennett, C.F., Chan, H., Nickoloff, B.J. J. Invest. Dermatol. (1994) [Pubmed]
  34. Effect of antisense oligodeoxynucleotides for ICAM-1 on renal ischaemia-reperfusion injury in the anaesthetised rat. Kiew, L.V., Munavvar, A.S., Law, C.H., Azizan, A.N., Nazarina, A.R., Sidik, K., Johns, E.J. J. Physiol. (Lond.) (2004) [Pubmed]
WikiGenes - Universities