Disease relevance of carboxymethyl-dimethyl-sulfonium

• An active photosystem I (PSI) complex was isolated from the thermophilic cyanobacterium Synechococcus elongatus by a procedure consisting of three steps: First, extraction of photosystem II from the thylakoids by a sulfobetaine detergent yields PSI-enriched membranes [1].
• We combined the use of low inoculation titers (300 +/- 100 CFU/ml) and enumeration of culturable cells to measure the osmoprotective potentialities of dimethylsulfoniopropionate (DMSP), dimethylsulfonioacetate (DMSA), and glycine betaine (GB) for salt-stressed cultures of Escherichia coli [2].
• Glycine betaine (GB) and its immediate precursors choline and carnitine, dimethylsulfonioacetate, dimethylsulfoniopropionate, ectoine and proline were effective osmoprotectants for Pseudomonas aeruginosa, but pipecolate, trehalose and sucrose had no osmoprotective effect [3].

High impact information on carboxymethyl-dimethyl-sulfonium

• Q-band electron nuclear double resonance (ENDOR) and X-band EPR of the sulfobetaine 12 heat-treated cytochrome c oxidase complex [4].
• The observation of sulfobetaine specifically bound to the protein active site was unexpected and is unique to this structure as far as we are aware [5].
• We prepared a washed cell membrane fraction that was insoluble in 134 mM nondetergent sulfobetaine and then extracted proteins using mixtures of detergents in a stepwise manner [6].
• The sulfobetaine 12 modified enzyme is no longer capable of undergoing the recently reported conformational transition in which the tryptophan fluorescence changes upon reduction of the low-potential metal centers [7].
• Despite the relatively low chaotropic nature of cations compared with anions, the retention data shows that cations are retained in this system predominantly due to a chaotropic interaction with the inner charge, analogous to anions in a system where the C18 column is coated with a sulfobetaine-type surfactant [8].

Biological context of carboxymethyl-dimethyl-sulfonium

• Furthermore, the zwitterionic and biomimetic nature of sulfobetaine and the ease of SBMA preparation make SBMA-based materials very attractive for a wide range of applications [9].
• They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics [10].

Anatomical context of carboxymethyl-dimethyl-sulfonium

• 5'-Nucleotidase from chicken gizzard smooth muscle has been extracted, using a sulfobetaine derivate of cholic acid, and purified to homogeneity by employing three chromatographic steps [11].
• Zwittergen (palmityl sulfobetaine), 2%, solubilizes the fusion protein that is found associated with inclusion bodies [12].

Associations of carboxymethyl-dimethyl-sulfonium with other chemical compounds

• One of the structures presents the crystallization additive sulfobetaine 195 (NDSB195) occupying simultaneously the ribose and phosphate binding sites, whilst a second presents only phosphate in the latter [5].
• Potential of the sulfobetaine detergent Zwittergent 3-12 as a desorbing agent in biospecific and bioselective affinity chromatography [13].

Gene context of carboxymethyl-dimethyl-sulfonium

• This type 2 copper EPR spectrum is identical to the EPR spectrum of CuB reported for the isolated cytochrome bo3 complex from Escherichia coli; and to the EPR spectrum reported for the sulfobetaine 12 heat-treated cytochrome c oxidase complex [14].

Analytical, diagnostic and therapeutic context of carboxymethyl-dimethyl-sulfonium

• The recombinant protein was purified to homogeneity by affinity chromatography and used for immunization after incorporation into liposomes and into micelles composed either of zwitterionic detergent or nondetergent sulfobetaine [15].
• Capillary electrophoresis (CE) using sulfobetaine-type zwitterionic micelles as the background electrolyte (BGE) has been used to determine inorganic anions in human saliva [16].
• Investigations into sulfobetaine-stabilized cu nanoparticle formation: toward development of a microfluidic synthesis [17].

References