High impact information on Adchem GMO

• In addition, MGAT activity was proportional to the level of MGAT1 protein expressed, and the amount of diacylglycerol produced depended on the concentration of either of its substrates, oleoyl-CoA or monooleoylglycerol [1].
• Based on the hydrolysis of specific monooleoylglycerol isomers, it was estimated that the 1(3)-ester bonds of the acylglycerols were hydrolyzed 3- to 4-fold faster than the 2-ester bonds [2].
• 4. This protein was further purified by hydrophobic chromatography on ethyl agarose; it was eluted with a micellar solution of sodium taurodeoxycholate, oleic acid, and monooleoylglycerol at pH 6 [3].
• Monooleoylglycerol caused a decrease in fluorescence from tryptophan-214 when measured at 350 nm while oleic acid had no effect on fluorescence at this wavelength and did not compete with monooleoylglycerol [4].
• The concentration-dependence of MAGL activity with monooleoylglycerol, the preferred substrate showed kinetics typical of an interfacial lipolytic enzyme displaying optimal activity on emulsified substrate particles; apparent Km values were 0.27 mM and 0.49 mM for the sn-1(3)- and sn-2-isomers respectively [5].

Biological context of Adchem GMO

• Membrane proteins in human erythrocytes during cell fusion induced by oleoylglycerol [6].
• Semisolid SEDDS formulations, composed of Peceol and Gelucire 44/14, produced bioavailability similar to the emulsion formulation [7].

Anatomical context of Adchem GMO

• Temperature-dependent structural changes in planar bilayer membranes formed from glycerol monooleate (GMO) dispersed in various n-alkane solvents (C12-C17) have been studied using precise measurements of specific geometric capacitance (Cg) [8].
• Influence of monooleoylglycerol on islet cell phosphoinositide hydrolysis and insulin secretion [9].
• It was calculated that the esterases account for approx. 2/3 of the monooleoylglycerol hydrolase activity in epithelial cells [10].
• The carboxylesterase (carboxylic-ester hydrolase, EC 3.1.1.1) and monoacylglycerol lipase (glycerol-monoester acylhydrolase, EC 3.1.1.23) activities, measured against ethyl butyrate and emulsified monooleoylglycerol respectively, were determined for chicken liver microsomes and highly purified chicken liver carboxylesterase [11].
• A significant lower mdr-1 mRNA and PGP protein expression within Caco-2 cells was observed following 1 and 7 days treatment with Peceol 0.1% to 1.0% (v/v) compared to nontreated controls [12].

Associations of Adchem GMO with other chemical compounds

• The relationship between triacylglycerol and monoacylglycerol hydrolyzing activities of purified rat heart lipoprotein lipase was studied using emulsified trioleoylglycerol and micellar or albumin-bound monooleoylglycerol as substrates [13].
• Using X-ray diffraction measurements and polarizing microscopy, the solubilization of ubiquinone-10 (UQ10) was investigated in the lamellar and reversed bicontinuous cubic phases of aqueous monoolein (MO, 86 wt% of monooleoylglycerol) [14].
• Influence of staurosporine, nitrendipine and monooleoylglycerol on interleukin-1-induced insulin secretion and phosphoinositide hydrolysis [15].
• Mixed micelles contained 2.5 mM monooleoylglycerol, 7.5 mM oleic acid, 12 mM sodium taurocholate and 200 microM lutein either with 3 mM PC or lysoPC [16].
• In order to enhance the effect of the TAG on the phase transitions in the GMO/triglyceride/water systems, temperature and electrolytes effects were examined [17].

Gene context of Adchem GMO

• Presence of double bond in acyl chain, as exemplified by monooleoylglycerol or mono-11-eicosenoin, further enhanced the conversion by GPI-PLD [18].
• Activity was low with the 1(3)- and sn-2-ether analogs of monooleoylglycerol, supporting the conclusion that the cells express the hepatic isoenzyme of MGAT [19].
• PURPOSE: To study the effects of two lipid excipients, Peceol and Gelucire 44/14 on the in vitro pancreatic lipase activity [20].
• It was found that TAG molecules with very short acyl chains (triacetin) can hydrate the head groups of the lipid and do not affect the critical packing parameter (CPP) of the amphiphile; therefore, they do not affect the self-assembly of the GMO in water, and the mesophase remains lamellar or cubic [17].

Analytical, diagnostic and therapeutic context of Adchem GMO

• The liquid-crystalline phases of the systems monooleoylglycerol (MO)/water, dioleoylphosphatidylcholine (DOPC)/water, and MO/DOPC/water have been studied by Fourier-transform infrared (FTIR) spectroscopy [21].
• 3. MG-lipase is more resistant to detergent and heat inactivation than TG-lipase and phospholipase A. It is retained, using the technique of affinity chromatography, on a column of CH-Sepharose coupled to monooleoylglycerol [22].

References