Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

PubChem9413 3,5,7,8,9- pentazabicyclo[4.3.0]nona- 2,4,6...

Synonyms: zlchem 203, SureCN24639, SureCN24640, ACMC-1BQHN, AG-D-31537, ...

Woods, R.A. et al., Busch, D.B. et al., Chen, D.J. et al., Spector, E.B. et al., Xiong, X. et al., et al.

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High impact information on NSC 32797

This paper describes the enhanced separation of adenine (A), hypoxanthine (HX), 8-azaadenine (8-AA), thymine (T), cytosine (C), uracil (U) and guanine (G) by CZE dispersing carboxylic multiwalled carbon nanotubes (c-MWNTs) into the running buffer [1].
The survival of MNNG- and MNU-resistant clones as well as of the parental CHO cell line was analysed after exposure to purine analogues substituted in different positions, 8-azaguanine (8-AG), 8-azaadenine (8-AA) and 6-thioguanine (6-TG) [2].
In this study, rare APRT heterozygotes were directly isolated from the TK6 population by clonal selection in cell culture medium supplemented with 5 micrograms/ml of 8-azaadenine [3].
Mutant UV140, in UV CG4, has approximately 3.8 x the UV sensitivity of parental line AA8, approximately 1.6 x wild-type MMC sensitivity, wild-type X-ray and ethyl methanesulfonate (EMS) sensitivity, and is only slightly (approximately 1.4 x) hypermutable to 8-azaadenine resistance by UV light [4].
Spontaneous and ethyl methanesulfate induced mutants of Saccharomyces cerevisiae, with partial and complete deficiency of adenine phosphoribosyltransferase (APRT, EC 2.4.2.7), were isolated by selection for resistance to 8-azaadenine [5].

Anatomical context of NSC 32797

Mutagenized stem-cell cultures were selected for resistance to 8-azaadenine and four clonal cell lines were isolated [6].

Associations of NSC 32797 with other chemical compounds

A chinese hamster ovary (CHO) cell line heterozygous at the adenine phosphoribosyl transferase (APRT) locus was used for selection of induced mutants resistant to 8-azaadenine (8AA), 6-thioguanine (6TG), ouabain (OUA), emetine (EMT) and diphtheria toxin (DIP) [7].
Cells with 30--40% of wild-type APRT activity were selected by resistance to 0.01 mM 2,6-diaminopurine or 1.40 mM 8-azaadenine [8].
The APRT in the 8-azaadenine-resistant cells did not have an altered mobility in glycerol gradients as compared to wild-type cells [8].

Gene context of NSC 32797

Mutants of Saccharomyces cerevisiae deficient in adenine phosphoribosyltransferase (A-PRT, EC 2,4,2,7) have been isolated following selection for resistance to 8-azaadenine in a prototrophic strain carrying the ade4-su allele of the gene coding for amidophosphoribosyltransferase (EC 2,4,2,14) [9].

References

Enhanced separation of purine and pyrimidine bases using carboxylic multiwalled carbon nanotubes as additive in capillary zone electrophoresis. Xiong, X., Ouyang, J., Baeyens, W.R., Delanghe, J.R., Shen, X., Yang, Y. Electrophoresis (2006) [Pubmed]
Tolerance to methylnitrosourea-induced DNA damage is associated with 6-thioguanine resistance in CHO cells. Aquilina, G., Zijno, A., Moscufo, N., Dogliotti, E., Bignami, M. Carcinogenesis (1989) [Pubmed]
Isolation of an APRT heterozygote from TK6 human lymphoblasts: predominance of multi-locus loss of heterozygosity among spontaneous APRT-mutants. Pongsaensook, P., Smith, L.E., Grosovsky, A.J. Mutat. Res. (1997) [Pubmed]
Phenotypic heterogeneity in nucleotide excision repair mutants of rodent complementation groups 1 and 4. Busch, D.B., van Vuuren, H., de Wit, J., Collins, A., Zdzienicka, M.Z., Mitchell, D.L., Brookman, K.W., Stefanini, M., Riboni, R., Thompson, L.H., Albert, R.B., van Gool, A.J., Hoeijmakers, J. Mutat. Res. (1997) [Pubmed]
Mutants of Saccharomyces cerevisiae deficient in adenine phosphoribosyltransferase. Sahota, A., Ranjekar, P.K., Alfonzo, J., Lewin, A.S., Taylor, M.W. Mutat. Res. (1987) [Pubmed]
Mouse teratocarcinoma mutant clones deficient in adenine phosphoribosyltransferase and developmentally pluripotent. Reuser, A.J., Mintz, B. Somatic Cell Genet. (1979) [Pubmed]
Cytotoxic and mutagenic properties of shale oil byproducts II. Comparison of mutagenic effects at five genetic markers induced by retort process water plus near ultraviolet light in Chinese hamster ovary cells. Chen, D.J., Strniste, G.F. Environmental mutagenesis. (1982) [Pubmed]
Purine reutilization and synthesis de novo in long-term human lymphocyte cell lines deficient in adenine phosphoribosyltransferase activity. Spector, E.B., Hershfield, M.S., Seegmiller, J.E. Somatic Cell Genet. (1978) [Pubmed]
Adenine phosphoribosyltransferase mutants in Saccharomyces cerevisiae. Woods, R.A., Roberts, D.G., Stein, D.S., Filpula, D. J. Gen. Microbiol. (1984) [Pubmed]

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