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Gene Review

APT1  -  adenine phosphoribosyltransferase APT1

Saccharomyces cerevisiae S288c

Synonyms: APRT 1, Adenine phosphoribosyltransferase 1, YML022W
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High impact information on APT1

  • These results demonstrate, for the first time, the refolding activity of Ssa1/2p in the context of the yeast cytosol, and define refolding activity as a chaperone function specific to Ssa1/2p, aprt from other cytosolic hsp70s [1].
  • We have used a hamster cell line engineered by gene targeting to contain a tandem duplication of the native adenine phosphoribosyltransferase (APRT) gene with an I-SceI recognition site in the otherwise wild-type APRT+ copy of the gene [2].
  • The behavior of adenine auxotrophs bearing additional mutations in purine salvage pathway genes (ade apt1, ade aah1 apt1, ade hpt1) supports a model in which secretion of degradation products, uptake, and reutilization of these products is a signal between cells synchronizing the sporulation process [3].
  • We demonstrate that the catalytic activity of the protein encoded by the yeast open reading frame is similar to that of rat APT1, and we designate the protein S. cerevisiae Apt1p [4].
  • Yeast APRTase is a dimeric molecule, and each subunit is composed of a central five-stranded beta-sheet surrounded by five alpha-helices, a structural theme found in all known purine phosphoribosyltransferases [5].

Biological context of APT1


Associations of APT1 with chemical compounds


Other interactions of APT1

  • Mutants deficient in adenine aminohydrolase (EC 3,5,4,2) activity, aah1, and hypoxanthine:guanine phosphoribosyltransferase (EC 2,4,2,8) activity, hpt1, were used to synthesize the genotypes apt1 hpt1 aah+ and apt1 hpt+ aah1 [9].
  • Northern (RNA) studies using the AAH-, APRT-, and CDC3-coding regions indicated that AAH regulation was not mediated at the level of transcription or mRNA degradation [10].

Analytical, diagnostic and therapeutic context of APT1

  • Northern and Western blot analyses demonstrated that only APT1 was transcribed and translated under normal physiological conditions in yeast [6].


  1. The refolding activity of the yeast heat shock proteins Ssa1 and Ssa2 defines their role in protein translocation. Bush, G.L., Meyer, D.I. J. Cell Biol. (1996) [Pubmed]
  2. Repair of site-specific double-strand breaks in a mammalian chromosome by homologous and illegitimate recombination. Sargent, R.G., Brenneman, M.A., Wilson, J.H. Mol. Cell. Biol. (1997) [Pubmed]
  3. Evidence for cooperation between cells during sporulation of the yeast Saccharomyces cerevisiae. Jakubowski, H., Goldman, E. Mol. Cell. Biol. (1988) [Pubmed]
  4. Characterization of Saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for G protein alpha subunit deacylation in vivo. Duncan, J.A., Gilman, A.G. J. Biol. Chem. (2002) [Pubmed]
  5. Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae. Shi, W., Tanaka, K.S., Crother, T.R., Taylor, M.W., Almo, S.C., Schramm, V.L. Biochemistry (2001) [Pubmed]
  6. APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae. Alfonzo, J.D., Crother, T.R., Guetsova, M.L., Daignan-Fornier, B., Taylor, M.W. J. Bacteriol. (1999) [Pubmed]
  7. Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae. Alfonzo, J.D., Sahota, A., Deeley, M.C., Ranjekar, P., Taylor, M.W. Gene (1995) [Pubmed]
  8. Site directed mutagenesis of the Saccharomyces cerevisiae APT1 gene. A functional and enzymatic analysis. Crother, T.R., Taylor, M.W. Adv. Exp. Med. Biol. (1998) [Pubmed]
  9. Adenine phosphoribosyltransferase mutants in Saccharomyces cerevisiae. Woods, R.A., Roberts, D.G., Stein, D.S., Filpula, D. J. Gen. Microbiol. (1984) [Pubmed]
  10. Adenine deaminase and adenine utilization in Saccharomyces cerevisiae. Deeley, M.C. J. Bacteriol. (1992) [Pubmed]
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