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Gene Review

NCKAP1  -  NCK-associated protein 1

Homo sapiens

Synonyms: HEM2, KIAA0587, Membrane-associated protein HEM-2, NAP 1, NAP1, ...
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Psychiatry related information on NCKAP1


High impact information on NCKAP1

  • NAP-1-IgG did not compete with 125I-NAP-1 for binding to neutrophils, which suggests that IgG anti-NAP-1 is a molecular trap that prevents binding of NAP-1 to neutrophils after it diffuses from production sites into the circulation [2].
  • However, at pH 2.0 in 9 M urea approximately 15% of the total NAP-1 could be dissociated from the complex [2].
  • The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion [3].
  • The present data suggest that T cell receptor engagement provokes a rapid, tyrosine kinase- and actin-dependent transport of Nck-associated FasL-carrying lysosomes to the contact area [4].
  • The findings supporting a global conservation of molecular mechanisms for membrane protrusion have been further strengthened by the identification of plant homologs of upstream regulators of the complex such as PIR121, NAP125 and HSPC300 [5].

Biological context of NCKAP1


Anatomical context of NCKAP1

  • Neutrophil attractant protein-1-immunoglobulin G immune complexes and free anti-NAP-1 antibody in normal human serum [2].
  • The purpose of this study was to determine if NAP-1 interacted with other types of blood leukocytes [10].
  • Neutrophil attractant/activation protein-1 (NAP-1) was previously shown to attract human neutrophils, but not monocytes [10].
  • Here it was examined how NAP1 interacts with chromatin fibers isolated from HeLa cells [11].
  • Intradermal NAP-1 did not cause basophil or lymphocyte infiltration [12].

Associations of NCKAP1 with chemical compounds


Physical interactions of NCKAP1


Enzymatic interactions of NCKAP1

  • In addition, Nck-associated proteins are highly tyrosine phosphorylated and JNK reaches a higher level of activation when FGF-2 triggers DNA synthesis [14].

Analytical, diagnostic and therapeutic context of NCKAP1

  • The mobilities of the 125 kDa protein and Nap1 on SDS/PAGE were indistinguishable, and the 125 kDa protein was depleted from brain extract by preincubation with the Src homology 3 domain of Nck to which Nap1 binds [15].
  • Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean [8].
  • The ELISA for free NAP-1 used a monoclonal capture antibody that did not bind NAP-1-IgG [2].
  • The effect of NAP1 on the chromatin fiber structure was examined by scanning/atomic force microscopy [11].
  • NAP1 induced a reversible change toward an extended fiber conformation as demonstrated by sedimentation velocity ultracentrifugation experiments [11].


  1. Isolation of hNap1BP which interacts with human Nap1 (NCKAP1) whose expression is down-regulated in Alzheimer's disease. Yamamoto, A., Suzuki, T., Sakaki, Y. Gene (2001) [Pubmed]
  2. Neutrophil attractant protein-1-immunoglobulin G immune complexes and free anti-NAP-1 antibody in normal human serum. Sylvester, I., Yoshimura, T., Sticherling, M., Schröder, J.M., Ceska, M., Peichl, P., Leonard, E.J. J. Clin. Invest. (1992) [Pubmed]
  3. The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion. Mayor, T., Stierhof, Y.D., Tanaka, K., Fry, A.M., Nigg, E.A. J. Cell Biol. (2000) [Pubmed]
  4. The adaptor protein Nck interacts with Fas ligand: Guiding the death factor to the cytotoxic immunological synapse. Lettau, M., Qian, J., Linkermann, A., Latreille, M., Larose, L., Kabelitz, D., Janssen, O. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  5. The ARP2/3 complex: giving plant cells a leading edge. Mathur, J. Bioessays (2005) [Pubmed]
  6. Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease. Suzuki, T., Nishiyama, K., Yamamoto, A., Inazawa, J., Iwaki, T., Yamada, T., Kanazawa, I., Sakaki, Y. Genomics (2000) [Pubmed]
  7. Human Nck-associated protein 1 and its binding protein affect the metabolism of beta-amyloid precursor protein with Swedish mutation. Yamamoto, A., Behl, C. Neurosci. Lett. (2001) [Pubmed]
  8. Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean. Yoon, H.W., Kim, M.C., Lee, S.Y., Hwang, I., Bahk, J.D., Hong, J.C., Ishimi, Y., Cho, M.J. Mol. Gen. Genet. (1995) [Pubmed]
  9. Nucleosome assembly protein 1 exchanges histone H2A-H2B dimers and assists nucleosome sliding. Park, Y.J., Chodaparambil, J.V., Bao, Y., McBryant, S.J., Luger, K. J. Biol. Chem. (2005) [Pubmed]
  10. Leukocyte specificity and binding of human neutrophil attractant/activation protein-1. Leonard, E.J., Skeel, A., Yoshimura, T., Noer, K., Kutvirt, S., Van Epps, D. J. Immunol. (1990) [Pubmed]
  11. NAP1 modulates binding of linker histone H1 to chromatin and induces an extended chromatin fiber conformation. Kepert, J.F., Mazurkiewicz, J., Heuvelman, G.L., Tóth, K.F., Rippe, K. J. Biol. Chem. (2005) [Pubmed]
  12. Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1. Leonard, E.J., Yoshimura, T., Tanaka, S., Raffeld, M. J. Invest. Dermatol. (1991) [Pubmed]
  13. Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release. White, M.V., Yoshimura, T., Hook, W., Kaliner, M.A., Leonard, E.J. Immunol. Lett. (1989) [Pubmed]
  14. Functional Rac-1 and Nck signaling networks are required for FGF-2-induced DNA synthesis in MCF-7 cells. Liu, J.F., Chevet, E., Kebache, S., Lemaitre, G., Barritault, D., Larose, L., Crépin, M. Oncogene (1999) [Pubmed]
  15. Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac. Kitamura, Y., Kitamura, T., Sakaue, H., Maeda, T., Ueno, H., Nishio, S., Ohno, S., Osada, S., Sakaue, M., Ogawa, W., Kasuga, M. Biochem. J. (1997) [Pubmed]
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