Disease relevance of CREB3L4

• Future studies will confirm a possible relationship between AIbZIP and prostate cancer [1].
• RT-PCR showed CREB4 transcripts were found in lung carcinoma LX-1, colon adenocarcinoma CX-1, prastatic adenocarcinoma PC-3, colon carcinoma G1-112, and pancreatic adenocarcinoma G1-103 [2].
• Evaluation of AIbZIP and Cdc47 as markers for human prostatic diseases [3].

High impact information on CREB3L4

• It codes for a 519 amino acid protein that contains a basic DNA binding and leucine zipper dimerization (B-ZIP) motif, highly similar to that in the OASIS, CREB-H, CREB4 and CREB3 transcription factors, followed by a hydrophobic region predicted to be an alpha-helical transmembrane domain [4].
• The highly tissue-specific expression profile, androgen regulation, chromosomal localization, and expression profile of AIbZIP in prostate tumors suggest that AIbZIP may play an important role in prostate cancer and in androgen receptor signaling in prostate cells [1].
• The AIbZIP gene is localized on chromosome 1q21.3 and consists of 10 exons [1].
• The kinetics of AIbZIP mRNA up-regulation and the results of experiments with cycloheximide suggest that AIbZIP may be a delayed response gene [1].
• Immunoreactive AIbZIP protein was primarily detected in the cytoplasm of prostatic luminal epithelial cells [1].

Chemical compound and disease context of CREB3L4

• Comparison of the mRNA expression profiles of control and R1881-treated cultures of LNCaP human prostate cancer cells using cDNA subtraction led to the identification of a novel transcription factor that we named Androgen-Induced bZIP (AIbZIP) protein [1].

Biological context of CREB3L4

• AIbZIP is a 395 aa protein with homology to cyclic AMP-responsive element binding protein/activating transcription factor transcription factors [1].
• The CREB4 gene consisted of ten exons and nine introns and was mapped to chromosome 1q21.3 by means of a bioinformatics analysis [5].
• Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1 [6].

Anatomical context of CREB3L4

• Each factor localizes to the endoplasmic reticulum (ER), but a population of CREB4 was also observed in the Golgi [7].
• The expression pattern of the human CREB4 gene showed transcripts in prostate, brain, pancreas, skeletal muscle, small intestine, testis, leukocyte, and thymus, whereas in heart, lung, liver, kidney, placenta, spleen, ovary, and colon, specific bands of the transcript could not be detected [5].
• A developmental study using Northern hybridization and in situ hybridization analyses revealed that Atce1 transcripts begin to accumulate in testes of mice 24 d after birth, reflecting expression in mid/late round spermatids [8].
• Atce1: a novel mouse cyclic adenosine 3',5'-monophosphate-responsive element-binding protein-like gene exclusively expressed in postmeiotic spermatids [8].
• Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus [6].

Associations of CREB3L4 with chemical compounds

• Dithiothreitol induced more complete transport of CREB4 to the Golgi, but not cleavage [7].
• CREB4 was cleaved in a site-specific manner in response to brefeldin A disruption of the Golgi or by coexpression with S1P but only after deletion or substitution of its C-terminal lumenal domain [7].

Other interactions of CREB3L4

• Thus, S1P cleavage of CREB4 may be suppressed by a determinant in the C-terminal region [7].

Analytical, diagnostic and therapeutic context of CREB3L4

• In contrast to CREB3L4 in the human prostate, of which specific expression was detected, Creb3l4 transcripts in the mouse prostate could be detected only by RT-PCR [9].
• Examination of AIbZIP protein and mRNA expression in a series of transurethral resection of the prostate and needle biopsy specimens indicated that AIbZIP is expressed at higher levels in cancerous prostate cells compared with noncancerous prostate cells [1].
• Molecular cloning and characterization of a novel human cAMP response element-binding ( CREB) gene ( CREB4) [5].

References