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Gene Review

rol-6  -  Protein ROL-6

Caenorhabditis elegans

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High impact information on rol-6

  • We report that trans-splicing of the rol-6 gene can be completely suppressed simply by introducing a donor site into its 173 nt outron, at a site 50 nt upstream of the trans-splice site, thereby converting rol-6 into a conventional gene with a spliced intron near its 5' end [1].
  • Equivalent alterations in sqt-1 and rol-6 generally produced the same phenotypes, indicating that conserved amino acids in these two collagens have similar functions [2].
  • The amino acid sequence of the collagen encoded by rol-6 is more similar to that of the sqt-1 collagen than to any of the other ten C. elegans cuticle collagen sequences compared [3].
  • A C. elegans strain mutant in cuticular collagen (rol-6) had a pattern identical to that of wild type, but another morphological mutant (dpy-3) [corrected] and several mutants that differ in surface reactivity to antibody and lectins (srf mutants) also had striking differences in surface labeling patterns [4].
  • Although some seam cells retain the ability to fuse at the final larval stage, the resulting syncytium shows gaps and bifurcations, translating into anomalies in cuticular ridges (alae) produced by the syncytium. nhr-25 RNAi markedly enhances branching of the alae caused by a mutant cuticular collagen gene rol-6 [5].

Biological context of rol-6

  • Certain mutations in the rol-6 gene produce animals that have the right roller phenotype, i.e., they are twisted into a right-handed helix [3].
  • In controls, where the tubulin fusion gene was directly injected to obtain transformants without any marker DNA, the cellular staining pattern was close to the fusion gene expression observed with the rol-6 marker DNA [6].
  • Effect of the dpy-20 and rol-6 cotransformation markers on alpha-tubulin gene expression in C. elegans transformants [6].

Anatomical context of rol-6

  • For the rol-6 marker, the tubulin fusion gene was expressed in several neurons in the head and tail ganglia and a set of 38-39 ventral cord motor neurons along the body length of the animal during larval and adult development [6].

Associations of rol-6 with chemical compounds

  • The locations of cysteine residues flanking the Gly-X-Y repeat regions of rol-6 and sqt-1 are identical, but differ from those in the other collagens [3].
  • A recessive RRol mutation of rol-6 is a replacement of one of the same conserved arginines by histidine [7].

Regulatory relationships of rol-6

  • We found that RNAi of TPST-A suppressed the roller phenotype caused by mutations in the rol-6 collagen gene, suggesting that sulfation of collagen proteins may be important for proper organization of the extracellular cuticle matrix [8].

Other interactions of rol-6

  • The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen [3].


  1. Conversion of a trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site. Conrad, R., Liou, R.F., Blumenthal, T. EMBO J. (1993) [Pubmed]
  2. In vitro mutagenesis of Caenorhabditis elegans cuticle collagens identifies a potential subtilisin-like protease cleavage site and demonstrates that carboxyl domain disulfide bonding is required for normal function but not assembly. Yang, J., Kramer, J.M. Mol. Cell. Biol. (1994) [Pubmed]
  3. The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen. Kramer, J.M., French, R.P., Park, E.C., Johnson, J.J. Mol. Cell. Biol. (1990) [Pubmed]
  4. Cuticle surface proteins of wild type and mutant Caenorhabditis elegans. Blaxter, M.L. J. Biol. Chem. (1993) [Pubmed]
  5. Nuclear receptor NHR-25 is required for cell-shape dynamics during epidermal differentiation in Caenorhabditis elegans. Silhánková, M., Jindra, M., Asahina, M. J. Cell. Sci. (2005) [Pubmed]
  6. Effect of the dpy-20 and rol-6 cotransformation markers on alpha-tubulin gene expression in C. elegans transformants. Fukushige, T., Siddiqui, S.S. Transgenic Res. (1995) [Pubmed]
  7. Analysis of mutations in the sqt-1 and rol-6 collagen genes of Caenorhabditis elegans. Kramer, J.M., Johnson, J.J. Genetics (1993) [Pubmed]
  8. Requirement of tyrosylprotein sulfotransferase-A for proper cuticle formation in the nematode C. elegans. Kim, T.H., Hwang, S.B., Jeong, P.Y., Lee, J., Cho, J.W. FEBS Lett. (2005) [Pubmed]
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