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Gene Review

Pcnt  -  pericentrin (kendrin)

Mus musculus

Synonyms: AW476095, C86676, KEN, Pcnt2, Pericentrin, ...
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High impact information on Pcnt


Biological context of Pcnt

  • However, comparison of the published mouse pericentrin cDNA sequence to mouse genomic DNA sequences revealed two important differences: the stop codon present in the published mouse pericentrin cDNA is not found in the mouse genomic sequence, and the 3' end of the published mouse pericentrin cDNA is a fragment from a different mouse chromosome [3].
  • To resolve these discrepancies, we sequenced a mouse expressed sequence tag (EST) that corresponds to the 3' end for a 7.1-kb mouse pericentrin RNA encoded on chromosome 10 [3].
  • No candidate hereditary disorder for pericentrin deficiency/abnormality has yet been mapped in the most distal region of 21q; in addition the role of triplication of the pericentrin gene in the pathophysiology or etiology of trisomy 21 is currently unknown [4].
  • Spindles in pericentrin-overexpressing cells were disorganized and mispositioned, and chromosomes were misaligned and missegregated during cell division, giving rise to aneuploid cells [5].
  • The metaphase spindle at meiosis-II had incomplete pericentrin rings at both spindle poles [6].

Anatomical context of Pcnt

  • Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and motile cilia [2].
  • Pericentrin (Pcnt) is a giant coiled-coil protein known to mediate microtubule organization [7].
  • Furthermore, IVO oocytes showed a twofold increase in cytoplasmic microtubule organizing centers (MTOCs), and constitutive MTOC proteins (gamma-tubulin and pericentrin) were excluded from the first polar body [8].
  • Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells [9].
  • After expression of a GFP-tagged gammatub, we observe a weak fluorescence along the nuclear membrane, confirming the presence of gammatub at a low concentration relative to PC [10].

Associations of Pcnt with chemical compounds

  • Treatment of cells with 0.5 M NaCl dissociates gamma-tubulin from the centrosome and disrupts the association of phosphoinositide 3-kinase with pericentrin, but not gamma-tubulin [11].
  • Vanadate treatment, a known inhibitor of dynein-ATPase, resulted in meiotic arrest, constriction of the spindle pole, and an aggregation of pericentrin at the spindle poles [6].
  • To investigate these questions, we have examined the redistribution of the centrosomal proteins pericentrin (PC), gammatub, and ninein in the C2 muscle cell line [10].
  • To test which of the above mechanisms or combination of mechanisms are responsible we investigated the re-formation of microtubules after depolymerisation by nocodazole, using antibodies against pericentrin, gamma-tubulin, EB1, and tyrosinated alpha-tubulin [12].

Physical interactions of Pcnt


Other interactions of Pcnt

  • Like pericentrin, p-MARCKS staining at the MI spindle poles was asymmetric [14].
  • To define the basis for this interaction, pericentrin was coexpressed with cytoplasmic dynein heavy (DHCs), intermediate (DICs), and light intermediate (LICs) chains, and the dynamitin and p150(Glued) subunits of dynactin [5].

Analytical, diagnostic and therapeutic context of Pcnt

  • Immunogold electron microscopy demonstrates that Pcnt is on or near basal bodies at the base of cilia [2].
  • Immunofluorescence analysis confirmed preferential expression of the Pcnt protein in G(0) phase cells [7].
  • In situ hybridization analysis revealed preferential expression of Pcnt in quiescent G(0) phase cells throughout the embryo with an unexpectedly low expression level in proliferating cells, suggesting that Pcnt might not play an important role in mitotic proliferation [7].
  • Extensive northern blot analysis revealed that the pericentrin gene displays a complex expression pattern in both mouse and human: a 10-kb kendrin transcript is found in most tissues, whereas smaller transcripts are detected in a limited subset of tissues [3].
  • Immunoprecipitation of endogenous pericentrin also pulled down cytoplasmic dynein in untransfected cells [5].


  1. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Doxsey, S.J., Stein, P., Evans, L., Calarco, P.D., Kirschner, M. Cell (1994) [Pubmed]
  2. Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly. Jurczyk, A., Gromley, A., Redick, S., San Agustin, J., Witman, G., Pazour, G.J., Peters, D.J., Doxsey, S. J. Cell Biol. (2004) [Pubmed]
  3. The centrosomal proteins pericentrin and kendrin are encoded by alternatively spliced products of one gene. Flory, M.R., Davis, T.N. Genomics (2003) [Pubmed]
  4. Localization of a human homolog of the mouse pericentrin gene (PCNT) to chromosome 21qter. Chen, H., Gos, A., Morris, M.A., Antonarakis, S.E. Genomics (1996) [Pubmed]
  5. Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization. Purohit, A., Tynan, S.H., Vallee, R., Doxsey, S.J. J. Cell Biol. (1999) [Pubmed]
  6. Sorting and reorganization of centrosomes during oocyte maturation in the mouse. Carabatsos, M.J., Combelles, C.M., Messinger, S.M., Albertini, D.F. Microsc. Res. Tech. (2000) [Pubmed]
  7. Embryonic expression of pericentrin suggests universal roles in ciliogenesis. Miyoshi, K., Onishi, K., Asanuma, M., Miyazaki, I., Diaz-Corrales, F.J., Ogawa, N. Dev. Genes Evol. (2006) [Pubmed]
  8. Distinctions in meiotic spindle structure and assembly during in vitro and in vivo maturation of mouse oocytes. Sanfins, A., Lee, G.Y., Plancha, C.E., Overstrom, E.W., Albertini, D.F. Biol. Reprod. (2003) [Pubmed]
  9. Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. Mogensen, M.M., Mackie, J.B., Doxsey, S.J., Stearns, T., Tucker, J.B. Cell Motil. Cytoskeleton (1997) [Pubmed]
  10. Reorganization of microtubule nucleation during muscle differentiation. Bugnard, E., Zaal, K.J., Ralston, E. Cell Motil. Cytoskeleton (2005) [Pubmed]
  11. Phosphoinositide 3-kinase binds constitutively to alpha/beta-tubulin and binds to gamma-tubulin in response to insulin. Kapeller, R., Toker, A., Cantley, L.C., Carpenter, C.L. J. Biol. Chem. (1995) [Pubmed]
  12. Microtubule assembly in cultured myoblasts and myotubes following nocodazole induced microtubule depolymerisation. Musa, H., Orton, C., Morrison, E.E., Peckham, M. J. Muscle Res. Cell. Motil. (2003) [Pubmed]
  13. Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Sillibourne, J.E., Delaval, B., Redick, S., Sinha, M., Doxsey, S.J. Mol. Biol. Cell (2007) [Pubmed]
  14. Phosphorylated MARCKS: a novel centrosome component that also defines a peripheral subdomain of the cortical actin cap in mouse eggs. Michaut, M.A., Williams, C.J., Schultz, R.M. Dev. Biol. (2005) [Pubmed]
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