Disease relevance of Spink1

• We propose that PSTI-I prevents pancreatitis by inhibiting the activity of trypsin, rather than by reducing trypsinogen activation [1].
• Increased secretion of the pancreatic secretory trypsin inhibitor (PSTI-I, monitor peptide) during development of chronic pancreatitis in the WBN/Kob rat [2].

High impact information on Spink1

• Recently, we isolated a trypsin-sensitive cholecystokinin-releasing peptide (CCK-RP) from porcine and rat intestinal mucosa [3].
• Diazepam binding inhibitor is a potent cholecystokinin-releasing peptide in the intestine [4].
• Purification and sequencing of a trypsin-sensitive cholecystokinin-releasing peptide from rat pancreatic juice. Its homology with pancreatic secretory trypsin inhibitor [5].
• Complementary nucleotide sequence for monitor peptide, a novel cholecystokinin-releasing peptide in the rat [6].
• Regulation of the action of the novel cholecystokinin-releasing peptide diazepam binding inhibitor by inhibitory hormones and taurocholate [7].

Biological context of Spink1

• Both predicted amino acid sequences consisting of 79 amino acids, with the secretion signal peptide consisting of 18 and 23 amino acids for PSTI-I and PSTI-II, respectively [8].
• Effect of a high-protein diet on the gene expression of a trypsin-sensitive, cholecystokinin-releasing peptide (monitor peptide) in the pancreas [9].
• Southern blot analyses suggested that both PSTI-I and PSTI-II genes are single copy genes per haploid genome [8].
• Binding studies of (125)I-labeled PSTI-I with the putative intestinal receptor using isolated membranes indicated the presence of high-affinity binding sites (k(d) = 5 x 10(-8)M) [10].
• This peptide was reported to be a cholecystokinin-releasing peptide and to stimulate pancreatic enzyme secretion in the rat small intestine in response to food intake [11].

Anatomical context of Spink1

• In the small intestine, PSTI-I, also called 'monitor peptide', is thought to have a different role: PSTI-I competes with protein for activated trypsin [10].

Other interactions of Spink1

• Northern blot analyses showed that PSTI-I is expressed in the pancreas, whereas PSTI-II is expressed in the pancreas and the liver using the same promoter [8].

Analytical, diagnostic and therapeutic context of Spink1

• Rapid and selective cloning of monitor peptide, a novel cholecystokinin-releasing peptide, using minimal amino acid sequence and the polymerase chain reaction [12].
• In a biological assay system, injections of PSTI-I displayed monitor peptide activity by inducing a dose-dependent trypsinogen release from the pancreas [10].
• METHODS: We collected pancreatic juice from WBN/Kob and Wistar rats aged 6-36 weeks and measured PSTI-I (ELISA) and trypsin [2].

References